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Method for preparing neurodevelopmental defect rat model through 3-MA treatment in weaning period of rats

A rat model, 3-MA technology, applied in the field of psychiatric research, can solve the problems of poor experiment repeatability, many influencing factors, and poor effect, and achieve the effect of abnormal behavior, low cost, and good model stability.

Active Publication Date: 2022-03-29
河南省精神病医院
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Problems solved by technology

Our research group has also tried the "two-strike model" model many times, but the effect is not good, and the procedure is cumbersome, there are many influencing factors, and the repeatability of the experiment is not good.

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  • Method for preparing neurodevelopmental defect rat model through 3-MA treatment in weaning period of rats
  • Method for preparing neurodevelopmental defect rat model through 3-MA treatment in weaning period of rats
  • Method for preparing neurodevelopmental defect rat model through 3-MA treatment in weaning period of rats

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Embodiment

[0022] The invention provides a method for preparing a neurodevelopmental defect rat model by administering 3-MA to the weaning period of the rat, which is characterized in that it comprises the following steps:

[0023] Step 1: Give normal weaning rats intraperitoneal injection of autophagy inhibitor 3-MA for 7 consecutive days to prepare a rat model that inhibits autophagy, and detect Becelin, P62, and LC3Ⅱ in the control group and the rat model by Western blotting expression to test the occurrence of autophagy;

[0024] pass figure 1 It can be found that compared with the control rats, the contents of Becelin and LC3II in the rat model treated with 3-MA decreased, and the content of P62 increased, suggesting that 3-MA reduces the occurrence of autophagy.

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Abstract

The invention provides a method for preparing a neurodevelopmental defect rat model through treatment with 3-MA in a weaning period of rats, which comprises the following steps: performing intraperitoneal injection of an autophagy inhibitor 3-MA on the rats in a normal weaning period continuously for 7 days to prepare a rat model for inhibiting autophagy generation, and detecting autophagy generation through immunoblotting. Then verifying social barriers through a three-box social experiment, verifying whether the anxiety level of the rat model is increased through an open field experiment, an elevated cross labyrinth experiment and a black and white box experiment, verifying whether the rat model has memory impairment through a Y labyrinth experiment and a passive avoidance experiment, and performing Golgi body staining on the rat model and a control group; and the neuron and synaptic morphology of the hippocampus and the frontal lobe Through multiple experiments, it is found that in the weaning period, 3-MA treatment for preparing the rat model with the neurodevelopment defect has the advantages of being low in cost, short in modeling period, more obvious in model behavior abnormality and better in stability, and can be used for research of diseases such as schizophrenia and autism and research and development of new drugs.

Description

technical field [0001] The invention belongs to the technical field of psychiatric disease research, and in particular relates to a method for preparing a neurodevelopmental defect rat model by treating rats with 3-MA during the weaning period. Background technique [0002] A large number of studies have shown that maternal infection during pregnancy is a high-risk factor for mental disorders and neurological diseases, and the risk of their offspring suffering from schizophrenia, autism, bipolar disorder, and major depression is significantly increased. Based on epidemiological findings, the most commonly used model of neurodevelopmental disorders in basic research on mental illness is the rat model of maternal infection during pregnancy. However, the modeling cycle of this model is long, the success rate of the offspring model is low, and the model is unstable. In view of the poor effect of this model, researchers at home and abroad improved on the basis of this model and ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 苏玺常绪路李文强杨勇锋宋盟邵明龙吕路线
Owner 河南省精神病医院
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