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Sound-sensitive response nano-particles and application thereof

A nanoparticle and drug technology, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of inability to distinguish the edge of malignant tumors and tumor proliferation, and the suppression of DC cell immune effects, and achieve excellent acoustic sensitivity and uniform size , the effect of inducing death

Active Publication Date: 2022-03-25
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

③ Unable to distinguish the margin of the malignant tumor and the spread of the tumor
⑤ chemotherapy resistance
However, tumor cells have the means to inhibit the function of DCs or change the tumor microenvironment, so that the immune effect of DC cells is suppressed

Method used

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  • Sound-sensitive response nano-particles and application thereof
  • Sound-sensitive response nano-particles and application thereof
  • Sound-sensitive response nano-particles and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Embodiment 1 Preparation of a novel nanomaterial HA-P(IC) / COS-PpIX

[0090] 1. Experimental method

[0091] 1. Synthesis of protoporphyrin-chitooligosaccharide (COS-PpIX) micelles

[0092] Dissolve 60mg (0.03mmol) chitooligosaccharide (COS) and 40mg (0.33mmol) 4-dimethylaminopyridine (DMAP) in 20mL dimethyl sulfoxide (DMSO); 80mg (0.7mmol) N-hydroxybutyl Diimide (NHS), 80 mg 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) (0.42 mmol) and 20 mg protoporphyrin (PpIX) (0.04 mmol) were added separately in DMSO. The two solutions were mixed and stirred at room temperature in the dark until PpIX was completely reacted.

[0093] Dialyzed against deionized water (Mw=1000) for 3 days, and the product COS-PpIX was collected by lyophilization. Disperse 5 mg of COS-PpIX freeze-dried powder in 5 mL of tetrahydrofuran (THF) solution, and then add dropwise (dropwise addition can allow THF to completely evaporate to achieve self-assembly) 5 mL of deionized water under ultra-high...

Embodiment 2

[0102] Example 2 Ultraviolet-visible spectroscopy (UV-VIS) detects COS-PpIX micelles and PpIX

[0103] 1. Experimental method

[0104] Each get 2mL of COS-PpIX prepared in Example 1 and PpIX solution (wherein PpIX is dissolved in DMSO / H 2 O system) was placed in a quartz dish, and deionized water was used as a blank, and then the absorption peak was scanned with a Unico 2802 ultraviolet spectrophotometer, and the wave band was set at 250nm to 700nm.

[0105] 2. Experimental results

[0106] figure 2 It is the ultraviolet-visible absorption spectrum of COS-PpIX prepared in Example 1 and PpIX. It can be seen from the figure that the maximum absorption peak of the PpIX prepared in Example 1 is around 488nm. After grafting chitosan oligosaccharide, its maximum absorption peak moves to the left and appears near 380nm, indicating that the COS-PpIX prepared in Example 1 The aggregation of PpIX can be effectively and significantly reduced, which may be attributed to the weak π-π ...

Embodiment 3

[0107] Example 3 Nanoparticle Size and Zeta Potential Detect Synthetic Products COS-PpIX Micelles, P(IC) / COS-PpIX and HA-P(IC) / COS-PpIX

[0108] In order to understand the particle size and potential size of the nanomaterials prepared in Example 1, the micelles (COS-PpIX) of the ultrasonic response and the micelles (P( IC) / COS-PpIX) and the particle size of nanomaterials (HA-P(IC) / COS-PpIX) modified by hyaluronic acid functionalization,

[0109] 1. Experimental method

[0110] Take 2mL of the synthetic products COS-PpIX, P(IC) / COS-PpIX and HA-P(IC) / COS-PpIX diluted to an appropriate concentration as the sample to be tested, and detect the particle size and Zeta potential.

[0111] 2. Experimental results

[0112] The particle size test results of the samples are as follows image 3 As shown, the particle size of the COS-PpIX ultrasonically responsive micelles prepared in Example 1 is basically concentrated at about 180nm. After adsorbing the adjuvant, the particle size of ...

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PUM

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Abstract

The invention discloses sound-sensitive response nano-particles and application thereof. The particle size of the sound-sensitive response nano-particles is about 250nm, and the sound-sensitive response nano-particles are uniform in size and good in stability; after ultrasonic stimulation, toxicity of glioma cells can be obviously caused, green fluorescence can be observed in the cells, and the probe has excellent sound-sensitive characteristics and can effectively induce cell death; under the action of ultrasonic cavitation, tumor-associated antigens can be effectively released, and the antigens are observed to be successfully ingested and treated by immature dendritic cells and effectively promote the maturation of the dendritic cells, which is beneficial to the generation of subsequent induced immune response. The invention can provide a new way for effective treatment of glioma.

Description

technical field [0001] The invention relates to the technical field of nano-medicines, in particular to an acoustically responsive nano-particle and an application thereof. Background technique [0002] The brain is the most advanced part of the nervous system, leading all activities in the body, including information processing, perception, motor control, awakening, balance, motivation, and learning and memory functions. Malignant brain tumors refer to tumors that grow in the brain, commonly known as brain tumors, including primary brain tumors that originate from the brain parenchyma and secondary brain tumors that metastasize to the brain from other parts of the body. The patient does not heal after treatment, leaving sequelae, and the survival rate is extremely low. Currently, the treatment methods for brain tumors include surgical resection, radiotherapy, chemotherapy and combination therapy. These methods can prolong the survival time of patients to a certain extent....

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K39/39A61K39/00A61K47/69A61K47/61A61P35/00A61P37/04
CPCA61K41/0033A61K39/39A61K39/0011A61K47/6907A61K47/61A61P35/00A61P37/04B82Y5/00B82Y40/00A61K2039/55561A61K2039/575
Inventor 关燕清林丹敏文著
Owner SOUTH CHINA NORMAL UNIVERSITY
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