Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human glioblastoma cell line GWH04 as well as culture method and application thereof

A glioblastoma and cell line technology, applied in human glioblastoma cells, human glioblastoma cell line GWH04 and its culture field, can solve problems such as poor tumorigenicity and achieve good transformation. Staining, good application prospects, stable cell characteristics

Pending Publication Date: 2022-03-22
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, U87 preserved by ATCC has been confirmed to be not the same source cell as U87 originally established in Uppsala laboratory; U251 and U373 have been confirmed to be of the same source; HS683 and A172 are rarely used in basic research due to their poor tumorigenicity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human glioblastoma cell line GWH04 as well as culture method and application thereof
  • Human glioblastoma cell line GWH04 as well as culture method and application thereof
  • Human glioblastoma cell line GWH04 as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This embodiment discloses the establishment of human glioblastoma cell line GWH04, which includes the following steps:

[0040] 1.1 The source of the specimen:

[0041] The tumor tissue sample came from a 72-year-old female patient with glioma occupying the right temporal lobe. In November 2016, she was admitted to the hospital due to "headache for more than 10 days, which aggravated for 4 days". The mass in the lateral temporal lobe was considered to be a neoplastic lesion, accompanied by herniation of the right parahippocampus and subfalx cerebellum, and microscopic craniotomy was performed in our hospital. Postoperative pathological examination showed: (right temporal lobe) glioblastoma (WHO grade IV). Immunohistochemistry: GFAP(+), NEUN(-), Nestin(+), EMA(+), Syn(-), Olig2(+), MGMT(+), Ki67 30%, EGFR(+), p53( +), C-myc (foci +). Sign the patient's informed consent before the operation. The patient's preoperative CT and MR imaging findings were figure 1 . For t...

Embodiment 2

[0051] This example discloses the biological characteristics of the human glioblastoma cell line GWH04.

[0052] 2.1 Morphological observation of GWH04 cell line

[0053] The subcultured GWH04 cells were taken, and the growth of living cells was observed under the Japanese Olympus IMT-2 inverted microscope 100×. see results image 3 , which are the bright-field cell morphology pictures of GWH04 cell lines P0, P1, P2, P10, P20, and P40, respectively, by image 3 It can be seen that this cell line grows vigorously, with a clear background, few impurities, and the adherent growth cells are spindle-shaped.

[0054] 2.2 Study on growth kinetics of GWH04 cell line

[0055] Detection of cell proliferation ability: take GWH04, U87, GL15 cells in logarithmic phase growth, and prepare 2×10 4 / mL cell suspension, spread 96-well plate, 100uL / well, that is, 2×10 3 / well, cells grow for 5 days, medium is not changed during this period, 30 auxiliary wells per cell, used for 6 tests, 5 w...

Embodiment 3

[0073] This example discloses the application of the human glioblastoma cell line GWH04 having the above biological characteristics in establishing an animal model of glioma. Specifically, as in the above step 2.6 and Figure 5 It can be seen that human glioblastoma cells have a very strong ability to form subcutaneous and intracranial tumors in nude mice, and can successfully prepare animal models of glioblastoma. The experiment found that intracranial tumors formed within 30 days, and nude mice could develop obvious tumors. Symptoms: Subcutaneous tumors formed within 30 days, and tumors with a diameter of 10mm were evident.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a human glioblastoma cell line GWH04 as well as a culture method and application thereof, and belongs to the technical field of neural tumor cell lines. The human glioblastoma cell line GWH04, which is preserved in the China Center for Type Culture Collection (CCTCC) and has a preservation number of CCTCC NO.C202163, is obtained by culturing tumor tissues of a patient suffering from right side temporal lobe occupying glioblastoma. The human glioblastoma cell line GWH04 can be used for preparing the human glioblastoma cell line GWH04. The novel glioblastoma cell line can be stably subcultured in vitro, is good in nude mouse tumorigenicity, and can be used for researching a glioma generation mechanism and a drug resistance mechanism or screening therapeutic drugs and the like.

Description

technical field [0001] The invention relates to a human glioblastoma cell, which belongs to the technical field of nerve cell lines, in particular to a human glioblastoma cell line GWH04 and its culture method and application. Background technique [0002] Glioma is the most common intracranial malignant tumor, accounting for about 26.6% of all intracranial tumors, of which glioblastoma (WHO grade IV) accounts for 56.1% of all gliomas, accounting for 47.1% of all intracranial malignant tumors %, the median survival time is about 14 to 16.6 months, and only about 5.5% of patients survive for 5 years through various treatments such as surgery, radiotherapy and chemotherapy. Even low-grade gliomas with relatively good prognosis (WHO grade II) will inevitably relapse and progress to high-grade gliomas (WHO grades III and IV) and eventually swallow up the patient's life. Regarding the treatment of glioblastoma, surgery is the main method. Even if postoperative adjuvant concurren...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/079C12Q1/02A01K67/027C12R1/91
CPCC12N5/0693C12N5/0622G01N33/5011A01K67/0271C12N2509/10C12N2509/00C12N2503/02G01N2500/10A01K2207/12A01K2227/105A01K2267/0331
Inventor 程芳玲万学焱毛峰王宝峰郭东生
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com