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Hydrated acellular skin substitute material and manufacturing method thereof

A manufacturing method, cell-free technology, applied in skin transplantation, medical science, prosthesis, etc., can solve problems such as immune response, damage to the dermis, transplant rejection, etc., and achieve the effect of soft physical properties

Active Publication Date: 2022-03-15
爱恩斯生物科技(昆山)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]However, in the manufacturing method used to obtain acellular dermis, the method using proteolytic enzymes can damage the dermis itself, if this enzyme remains and is transplanted into In vivo, it can damage the patient's original tissue, and in severe cases, it can cause an immune response, so that the transplant may be rejected
In addition, the disadvantage of the cell-free method using surfactants among the commonly used methods is that a lot of cleaning time and cost must be invested in order to minimize the residue of surfactants

Method used

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  • Hydrated acellular skin substitute material and manufacturing method thereof
  • Hydrated acellular skin substitute material and manufacturing method thereof
  • Hydrated acellular skin substitute material and manufacturing method thereof

Examples

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Embodiment approach

[0081] Hereinafter, the present invention will be described more specifically by way of examples. However, the scope of the present invention is not limited to the following examples, and those skilled in the art should understand that various variations, modifications, or applications can be made within the scope of the technical idea derived from the contents of the claims.

Embodiment 1

[0083] Example 1. Manufacture of acellularized skin substitute material

[0084] (1) Skin pretreatment process

[0085] Skin tissue was prepared (collected from tissue banks, cadavers donated for non-profit purposes and used to treat patients).

[0086] Put the cleaned skin tissue on a sterile table, and use a scraper to remove fascial tissue, adipose tissue, and other foreign objects attached to the dermis until the dermis is partially visible. Use a sterile spatula to remove as much of the remaining fat as possible in the dermis, and use sterile scissors and forceps to remove the remaining unnecessary tissue. Wash several times with sterile distilled water.

[0087] (2) Fat removal process

[0088] The degreasing solution is made by mixing IPA and hexane at a mixing ratio of IPA:hexane of 2:8 to 8:2.

[0089] The degreasing solution and the pretreated skin tissue were put into a 4L jar, and stirred at room temperature at 150 rpm for 2 hours. Remove the skin tissue durin...

experiment example 1

[0103] Experimental example 1. Confirm whether the collagen fibers in each step are damaged

[0104] After each step of Example 1, it was confirmed whether the collagen fibers of the skin tissue were damaged.

[0105] (1) Confirm whether the collagen fibers are damaged after the fat removal process

[0106] After performing the (2) fat removal process of Example 1, the skin tissue was photographed using a scanning electron microscope (SEM).

[0107] figure 1 It shows the result of photographing with a scanning electron microscope (SEM) to confirm whether the collagen fibers are damaged after the treatment of the degreasing solution.

[0108] Such as figure 1 As shown, it was confirmed that no tissue deformation or collagen fiber damage was observed in the skin (IPA / hexane) from which fat was removed using a degreasing solution, as in the raw skin material (raw skin).

[0109] (2) Confirm whether the collagen fibers are damaged after the cell removal process

[0110] After...

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Abstract

The invention relates to an acellular skin substitute material in a hydrated state and a manufacturing method thereof. In the present invention, an acellular dermis is obtained by excluding an acellular process using a surfactant and an enzyme, and an acellular skin substitute material imparted with soft characteristics can be produced by treatment in a sodium sulfide solution and immersion in a biogenic polymer solution.

Description

technical field [0001] The present invention relates to a hydrated acellularized skin substitute material and a manufacturing method thereof. Background technique [0002] Acellular Dermal Matrix (ADM) refers to an extracellular matrix (ECM) composed of pure collagen, elastin, etc. Morphology of bioderived skin substitute materials. It is known that, unlike organs for transplantation, such bioderived skin replacement materials have relatively low immune rejection. There are also differences in the degree of immune rejection when human-derived skin substitute materials and animal-derived skin substitute materials are transplanted, but it has been reported that human-derived skin substitute materials are generally more biologically and safely than animal-derived skin substitute materials. Alternative materials are superior. However, human-derived skin substitute materials require skin tissue donation from post-mortem donors, so there may be limitations in raw material suppl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
CPCA61L27/60A61L2430/40A61L27/36A61F2/10A61L27/3687A61L27/362A61F2/105
Inventor 李索李誾成李基源金炯九李焕铁
Owner 爱恩斯生物科技(昆山)有限公司
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