Glucoregulatory protein 78 targeting nano antibody and application thereof

Abstract

The invention provides a nano antibody targeting glucose regulatory protein 78 and application thereof. Complementary determining regions of an amino acid sequence of the nano antibody targeting glucose regulatory protein 78 are CDR1 as shown in SEQ ID NO: 2, CDR2 as shown in SEQ ID NO: 4 and CDR3 as shown in SEQ ID NO: 6; the CDR1 is as shown in SEQ ID NO: 9, the CDR2 is as shown in SEQ ID NO: 11, and the CDR3 is as shown in SEQ ID NO: 13. The affinity of the nano antibody targeting the glucose regulatory protein 78 and the glucose regulatory protein 78 is at a nanomole level, the affinity is high, and the nano antibody has specific recognition capacity on the GRP78 protein on the surface of a cell membrane.

Description

technical field [0001] The invention belongs to the technical field of immunology or molecular biology, and in particular relates to a nanobody targeting glucose regulatory protein 78 and its application. Background technique [0002] Glucose regulated protein 78 kDa, GRP78, is a protein with a molecular weight of 78 kDa discovered by IraPastan et al. in chicken embryo fibroblasts cultured without glucose in 1977. It binds to the immunoglobulin heavy chain (binding immunoglobulin protein, BIP) is exactly the same, so it is also called immunoglobulin heavy chain binding protein (BiP). , metastasis, and angiogenesis are critical. The full length of the GRP78 protein consists of two functional domains, including a nucleotide-binding domain (NBD), which can bind ATP, and a substrate-binding domain (SBD), which can bind peptides / proteins . GRP78 is widely present in the cytoplasm, but under stress conditions such as hypoxia, glucose deficiency, and pH drop in the tumor microen...

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Problems solved by technology

However, so far, there have been no relevant reports on nanobodies with good binding and high affinity to GRP78 protein.

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