Sample density separation medium and cell separation method

Abstract

The invention relates to the technical field of cytological flaking, in particular to a sample density separation medium and a cell separation method. The sample density separation liquid provided by the invention is prepared from a solvent, disodium ethylene diamine tetraacetate, disodium hydrogen phosphate, monopotassium phosphate, potassium chloride, sodium chloride, dithiothreitol, tea saponin and sodium sulfite. The mucus in the sample can be effectively softened and decomposed, the detection background is clear and clean, and the cell morphology is clearly observed.

Description

technical field [0001] The invention relates to the technical field of cytology, in particular to a sample density separation liquid and a cell separation method. Background technique [0002] Cervical cancer is currently the only cancer with a clear cause that can be detected and prevented early. Therefore, early screening of cervical cancer is of great significance to the prevention of cervical cancer. Liquid-based cytometry, TCT for short, is a new cytology film production technology introduced in recent years, and it is a major innovation in the field of film production. Compared with traditional smears, liquid-based cytology preserves specimens through preservation solution, and then undergoes a series of pre-processing techniques to remove impurities in the specimens and make a thin layer that can be observed clearly under the microscope, making it easier for readers to observe. Its diagnostic accuracy and sensitivity are significantly improved compared with tradition...

AI Technical Summary

Problems solved by technology

[0004] The main component of the existing sample density separation liquid is a synthetic sucrose polymer, referred to as polysucrose, but after adding the density separation liquid to the preservation liquid, the unresolved particles at the layered part of the preservation liquid and the sample density separation liquid The red blood cells that are completely lysed in the preservation solution will be in contact with polysucrose to condense into a string of money and deposit at the bottom of the tube and mix with the sinking cells of diagnostic significance (epithelial cells, tumor cells), which will affect the observation of the sample after subsequent film production

In addition, the traditional sample density separation fluid does not have the function of removing mucus, and because the mucus wraps the cells, it cannot be separated by density centrifugation, which also affects the final production effect

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