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Aspergillus fumigatus strain and application thereof

A technology of Aspergillus fumigatus and strains, applied in the field of microorganisms, can solve the problems of not being easy to use effectively, occupying land resources, heavy metal pollution, etc., and achieve the effects of improving the ability to absorb phosphorus, increasing the phosphorus absorption coefficient, and relieving pressure

Pending Publication Date: 2022-03-01
中国烟草总公司贵州省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coal gangue produced in various coal production areas is basically stored in open-air accumulation, and a large amount of open-air coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; coal gangue weathering and spontaneous combustion will release toxic gases, resulting in Dust, causing heavy metal pollution; rainwater washes coal gangue, causing changes in the composition of coal gangue to produce toxic substances into the soil, polluting water quality
In short, the massive accumulation of coal gangue has caused waste of resources and serious environmental pollution, so it is of great significance to develop new resource utilization methods for coal gangue.
[0003] The composition analysis of coal gangue shows that coal gangue contains many nutrients needed for plant growth, such as phosphorus, potassium, calcium, nitrogen and organic matter, etc., but most of the phosphorus, potassium and other components contained in coal gangue are insoluble minerals. Exist in form, not easy to be effectively used

Method used

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  • Aspergillus fumigatus strain and application thereof
  • Aspergillus fumigatus strain and application thereof
  • Aspergillus fumigatus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation and screening of functional strains

[0035] 1) Collection of samples

[0036] (1) The samples were collected from coal gangue hills and nearby soil in a coal production area in Guizhou. Select the soil at the root of the plant that grows well, first shovel off the surface soil, dig 10-20cm along the root of the plant, and use the "root shaking method" to collect the soil in a sterile ziplock bag.

[0037] (2) The ice box is sealed and taken back to the laboratory. After removing the plant residues, crush them through a 20-mesh sieve.

[0038] (3) Store the samples in a refrigerator at 4°C for refrigerated storage.

[0039] 2) Screening of functional strains

[0040] (1) Accurately weigh 1 g of the sample into a sterilized 250 mL Erlenmeyer flask, and add 99 mL of sterile water.

[0041] (2) Shake for 30 minutes in a constant temperature shaker at 170r / min and around 28°C.

[0042] (3) After shaking for 30 minutes, take the supernatant and dilu...

Embodiment 2

[0049] Embodiment 2: the drawing of the growth curve of functional bacterial strain

[0050] 1) Preparation of fungal spore suspension: use an inoculation needle to pick the mycelia of the fungus to be tested on the PDA slant medium, activate at 30°C for 36-72 hours, take out after a large number of fungal spores grow, and use 5mL sterile distilled water on the ultra-clean workbench Rinse the slope, make a suspension of fungal spores, dilute to 100mL, shake well and set aside.

[0051] 2) Inoculation and cultivation: Pour 100mL LB liquid medium into a 250mL Erlenmeyer flask, prepare a total of 30 bottles, put them into an autoclave for sterilization at 121°C for 30min, and inoculate the prepared fungal spore suspension at 5% Add the amount into the sterilized LB liquid medium, 170r·min -1 , 30 ℃ shaker cultivation. Mark the bacteria number and culture time on the Erlenmeyer flask.

[0052] 3) Determination: Take out 3 bottles every 24 hours, dry the blank filter paper at 10...

Embodiment 3

[0054]Embodiment 3: the identification of functional bacterial strain

[0055] In the present invention, the fungus GZT-Asp01 entrusts China Center for Type Culture Collection to identify it.

[0056] 1) Colony appearance

[0057] The primary hyphae of the fungus GZT-Asp01 are white, hairy, protruding in the middle of the colony, and spread radially. After 5 days, smoke green spores began to appear in the middle of the colony. After about 9 days, the mycelium can cover the entire plate, and the culture can be seen from the back of the plate. The basal matrix turns yellowish brown, such as figure 2 shown.

[0058] 2) Microscopic morphology of fungi

[0059] The GZT-Asp01 fungus was stained with safranin, and the microscopic morphology of the fungus was observed under an optical microscope after staining. It can be observed with an optical microscope that the hyphae of the GZT-Asp01 fungus are transparent, and the top of the conidiophores swells into an apical capsule, whic...

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Abstract

The invention discloses an aspergillus fumigatus strain and application thereof. The aspergillus fumigatus strain is preserved in the China Center for Type Culture Collection on June 25, 2021, the preservation number is CCTCC NO: M 2021784, and the aspergillus fumigatus strain is classified and named as aspergillus fumigatus GZT-Asp01. The strain aspergillus fumigatus GZT-Asp01 can dissociate phosphorus, potassium, calcium, nitrogen and other components in the coal gangue and convert the components into available phosphorus, quick-acting potassium, exchangeable calcium, hydrolytic nitrogen and other nutritional components which can be absorbed by plants, and especially can effectively dissociate insoluble phosphorus and / or insoluble phosphorus in the coal gangue; and the phosphorus-dissolving effect of the strain on coal gangue is superior to that of the traditional phosphorus-dissolving strain such as bacillus megatherium.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Aspergillus fumigatus and its application. Background technique [0002] my country is a large country of coal production, and coal occupies an important position in my country's energy structure. A large amount of coal gangue is produced in the process of coal mining and washing, and its output accounts for 20%-35% of coal output. Since coal gangue has not been effectively utilized for a long time, it has become the largest industrial solid waste. Coal gangue produced in various coal production areas is basically stored in open-air accumulation, and a large amount of open-air coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; coal gangue weathering and spontaneous combustion will release toxic gases, resulting in Dust, causing heavy metal pollution; rain washes coal gangue, resulting in changes in the composition o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/68C09K101/00
CPCC12N1/14C09K17/14C09K2101/00
Inventor 谢承卫吉俐李小军盛定红
Owner 中国烟草总公司贵州省公司
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