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Heterojunction functionalized chlorella as well as preparation method and application thereof

A chlorella, functional technology, applied in the field of biomedicine, can solve the problem of limiting the effective production of ROS, and achieve the effect of weakening the antioxidant capacity, increasing the curative effect, and enhancing the effect of immunotherapy

Active Publication Date: 2022-03-01
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the tumor microenvironment is usually hypoxic, which limits the effective generation of ROS, and the immune effect generated by PDT treatment alone is not sufficient to effectively clear residual tumor cells and inhibit metastasis.

Method used

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  • Heterojunction functionalized chlorella as well as preparation method and application thereof
  • Heterojunction functionalized chlorella as well as preparation method and application thereof
  • Heterojunction functionalized chlorella as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation of Chl@BP-Fe

[0053] S1. Preparation of black phosphorus nanosheets (BPNSs): Grind 40 mg of black phosphorus for 30 min, add 80 mL of N-methylpyrrolidone solvent, ultrasonically crush at a power of 700 W for 8 h, then sonicate in a water bath for 8 h, and centrifuge at a speed of 7000 rpm for 10 min. Supernatant, centrifuge the supernatant at 15000rpm for 15min, take the precipitate, and obtain BPNSs;

[0054] S2. Preparation of black phosphorus nanosheets (BP / PASP-Fe) with surface-modified polyaspartic acid and chelated iron ions: 1 μL of sodium polyaspartate aqueous solution with a mass concentration of 0.4 g / mL and step S1 to obtain 0.2mg of black phosphorus nanosheets were mixed evenly, vortexed for 1min, and then 62.5μL of FeCl with a concentration of 24mmol / L was added 3 The solution was mixed evenly, vortexed for 1 min, washed 3 times with PBS, and centrifuged at 15,000 rpm for 10 min to obtain the "Lego building block" module BP / PASP-Fe; ...

Embodiment 2

[0056] Example 2 Preparation of Chl@BP-Fe

[0057] S1.Preparation of BPNSs: Grind 10 mg of black phosphorus for 45 min, add 100 mL of N-methylpyrrolidone solvent, ultrasonically crush at 600 W for 12 h, then sonicate in a water bath for 12 h, centrifuge at 8000 rpm for 15 min, take the supernatant, and The supernatant was centrifuged at 14000rpm for 20min, and the precipitate was collected to obtain BPNSs;

[0058] S2. Preparation of BP / PASP-Fe: Mix 0.5 μL of an aqueous solution of sodium polyaspartate with a mass concentration of 0.4 g / mL and 0.1 mg of BPNSs obtained in step S1, and then add 57 μL of FeCl with a molar concentration of 22 mmol / L 3 The solution was mixed evenly and vortexed for 1 min, washed 3 times with PBS, and centrifuged at 15,000 rpm for 20 min to obtain the "Lego building blocks" module BP / PASP-Fe;

[0059] S3. Preparation of Chl@BP-Fe: Mix the BP / PASP-Fe obtained in step S2 with the chlorella cell suspension by vortexing for 1 min, wherein the ratio of ...

Embodiment 3

[0060] Example 3 Preparation of Chl@BP-Fe

[0061] S1. Preparation of BPNSs: Grind 200 mg of black phosphorus for 60 min, add 100 mL of N-methylpyrrolidone solvent, ultrasonically break at 650 W for 24 h, then sonicate in a water bath for 24 h, centrifuge at 7500 rpm for 12 min, take the supernatant, and The supernatant was centrifuged at 14800rpm for 18min, and the precipitate was collected to obtain BPNSs;

[0062] S2. Preparation of BP / PASP-Fe: Mix 2 μL of an aqueous solution of sodium polyaspartate with a mass concentration of 0.4 g / mL and 0.5 mg of BPNSs obtained in step S1, and then add 80.3 μL of FeCl with a molar concentration of 20 mmol / L 3 The solution was mixed evenly, vortexed for 1 min, washed 3 times with PBS, and centrifuged at 15,000 rpm for 10 min to obtain the "Lego building block" module BP / PASP-Fe;

[0063]S3. Preparation of Chl@BP-Fe: Mix the BP / PASP-Fe obtained in step S2 with the chlorella cell suspension by vortexing for 1 min, wherein the ratio of bla...

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Abstract

The invention discloses heterojunction functionalized chlorella. The heterojunction functionalized chlorella consists of chlorella cells and black phosphorus nanosheets which are loaded on the surfaces of the chlorella cells, are used for modifying polyaspartic acid and are chelated with iron ions. The black phosphorus nanosheet and an original photosynthetic system in chlorella cells are coupled to construct a type II heterojunction, the oxygen production capacity of chlorella photosynthesis and the photodynamic < 1 > O2 production capacity of the black phosphorus nanosheet are synchronously and synergistically enhanced, the tumor hypoxic microenvironment is relieved, and the curative effect is enhanced; cGF generated by chlorella cells causes activation of an immune system, promotes antigen recognition, presentation and immune response of the body immune system and enhances the immunotherapy effect, and Fe < 3 + > released by heterojunction functionalized chlorella enters tumor cells, can consume GSH and is reduced into Fe < 2 + >, so that the oxidation resistance of the tumor cells is weakened, and the immunotherapy effect is enhanced. The Fe < 2 + >-mediated Fenton reaction increases the curative effect of chemodynamic therapy on tumors.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a heterojunction functionalized chlorella and its preparation method and application. Background technique [0002] Photodynamic therapy (PDT) is a non-invasive tumor treatment method that selectively kills tumor cells and has obtained clinical approval. 1 o 2 ), superoxide anion (·O 2 - ), hydroxyl radicals (·OH), and reactive oxygen species (Reactive oxygen species, ROS) regulate necrosis and apoptosis of tumor cells, and are currently very effective means of cancer treatment. The therapy mainly uses a laser with a specific wavelength to excite a photosensitizer (Photosensitizer, PS) to transfer electrons or energy to O 2 Generate ROS, cause tumor cell death, microvascular damage, and induce local immune responses. Compared with traditional therapy, PDT has the advantages of targeted light source, selective killing of tumor cells; low toxicity, minimal invasiveness, an...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/69A61K47/64A61K47/52A61K36/05A61P35/00A61P35/04
CPCA61K36/05A61K47/645A61K47/52A61K41/0057A61K47/6901A61P35/00A61P35/04A61K2300/00
Inventor 姬晓元欧美彤梅林张锦勰
Owner SUN YAT SEN UNIV
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