Preparation method and application of photoelectrochemical biosensor for detecting beta-lactoglobulin

A biosensor, lactoglobulin technology, applied in the field of functional nanomaterials, can solve the problems of weak surface adsorption capacity, small specific surface area, poor photoelectric active electron hole separation ability, etc., and achieves good reproducibility, high sensitivity, and detection. quick effect

Pending Publication Date: 2022-02-15
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

wxya 4 It is an important optoelectronic active material due to its stability, suitable band gap and low cost, but its optoelectronic activity is limited by poor electron-hole pair separation ability, small specific surface area, and weak surface adsorption capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 prepares CdWO 4 ,Proceed as follows:

[0031] Take 1.0 mmol Cd(NO 3 ) 2 4H 2 O and 1.0 mmol Na 2 WO 4 2H 2 O was dissolved in 12.5 mL of deionized water and marked as A and B respectively; solution B was added dropwise to solution A under stirring, and after continuous stirring for 30 min, the mixture was transferred to a 50 mL polytetrafluoroethylene-lined stainless steel high-pressure The reaction was carried out at 180 °C for 24 h in an autoclave; after natural cooling to room temperature, the product was collected by centrifugation, washed with ethanol and deionized water three times each, and then vacuum-dried at 50 °C.

Embodiment 2

[0032] Embodiment 2 prepares CdWO 4 ,Proceed as follows:

[0033] Take 1.25 mmol Cd(NO 3 ) 24H 2 O and 1.25 mmol Na 2 WO 4 2H 2 O was dissolved in 12.5 mL of deionized water and marked as A and B respectively; solution B was added dropwise to solution A under stirring, and after continuous stirring for 30 min, the mixture was transferred to a 50 mL polytetrafluoroethylene-lined stainless steel high-pressure The reaction was carried out at 180 °C for 24 h in an autoclave; after natural cooling to room temperature, the product was collected by centrifugation, washed with ethanol and deionized water three times each, and then vacuum-dried at 50 °C.

Embodiment 3

[0034] Example 3 Preparation of CdWO 4 ,Proceed as follows:

[0035] Take 1.5 mmol Cd(NO 3 ) 2 4H 2 O and 1.5 mmol Na 2 WO 4 2H 2 O was dissolved in 12.5 mL of deionized water and marked as A and B respectively; solution B was added dropwise to solution A under stirring, and after continuous stirring for 30 min, the mixture was transferred to a 50 mL polytetrafluoroethylene-lined stainless steel high-pressure The reaction was carried out at 180 °C for 24 h in an autoclave; after natural cooling to room temperature, the product was collected by centrifugation, washed with ethanol and deionized water three times each, and then vacuum-dried at 50 °C.

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Abstract

The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting dairy allergen beta-lactoglobulin, and belongs to the technical fields of functional nano materials, food analysis and photoelectrochemical biosensing. A CdWO4-TiO2 heterojunction composite material is fixed on the surface of an ITO (Indium Tin Oxide) electrode, CdS quantum dots are used for sensitization to enhance absorption of light, the CdS quantum dots are used as a connecting agent to fix an allergen beta-lactoglobulin antibody, the photocurrent response and sensitivity are effectively improved, through a layer-by-layer assembly method, by utilizing the good photoelectric activity of the CdWO4-TiO2 heterojunction sensitized by the CdS quantum dots and the specific binding between the beta-lactoglobulin and the beta-lactoglobulin antibody, the ultra-sensitive detection of the beta-lactoglobulin is realized, and the method has important significance on the analysis and detection application of the beta-lactoglobulin in dairy products.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting dairy allergen β-lactoglobulin, and belongs to the technical fields of functional nanomaterials, food analysis and photoelectrochemical biosensing. CdWO 4 -TiO 2 The heterojunction composite material is immobilized on the surface of the ITO electrode, sensitized by CdS quantum dots to enhance its absorption of light, and used as a linker to immobilize the allergen β-lactoglobulin antibody, effectively improving the photocurrent response and sensitivity. Sensitization of CdWO with CdS quantum dots via a layer-by-layer assembly approach 4 -TiO 2 The good photoelectric activity of the heterojunction and the specific binding between β-lactoglobulin and β-lactoglobulin antibody can realize the ultra-sensitive detection of β-lactoglobulin, which is of great significance for the analysis and detection of β-lactoglobulin in dairy products. Significance. Backg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 陈志伟孔玲李灿国朱琪颖孟庆红常慧琴刘安琪吴宇银刘红亮
Owner SHANDONG UNIV OF TECH
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