Composition for treating hormone face and acne skin and preparation method thereof
A composition and acne technology, applied in the field of biology and new medicine, can solve the problems of poor treatment effect, lack of pertinence, long treatment time, etc., to enhance skin nutrition and water intake, reduce skin fine lines, Improvement effect of inflammatory skin
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Embodiment 1
[0032] Example 1 Preparation of Stem Cell Culture Supernatant
[0033] The stem cell culture supernatant was prepared from the umbilical cord, which was taken from the puerpera. The specific requirements for the puerpera are: (1) 36-42 weeks of pregnancy, normal development and nutrition; (2) no infectious diseases, a tube of 5mL maternal blood is required , Maternal blood test, EBV, HCMV, HIV, HBV, HCV, syphilis should be negative; (3) no malignant tumor; (4) no serious complications during pregnancy; (5) no family history of genetic diseases.
[0034] The umbilical cord obtained by the invention is uniform in appearance, smooth in surface, free of transparent colloid, free of wrinkle or edema, and elastic.
[0035] Clean and disinfect the biological safety cabinet according to the management regulations of aseptic technology, prepare the reagents and materials required for the experiment, wipe the outer surface of the bottle with 75% alcohol from the bottle cap down, and put...
Embodiment 2
[0056] Embodiment 2 is used for the preparation of the composition of treatment hormone face and acne muscle
[0057] In an environment of Class A cleanliness, the hyaluronic acid, glycerin, vitamin B, trehalose, and stem cell culture supernatant are respectively in accordance with the mass percentage: 1-3%, 2-6%, 0.1-2%, 2-20% %, 20-30% are calculated and weighed, and sterilized water for injection makes up 100%; then, after the above components are fully mixed, they are aseptically filled into 2ml sterile tubes without pyrogens.
Embodiment 3
[0058] The effect test of embodiment 3 composition
[0059] Randomly select 20 patients with hormonal face and acne skin that are difficult to be cured by existing methods, firstly disinfect the rolling needle with 75% alcohol (time: ≥20min), clean the face, use povidone iodine to disinfect the face, and then use normal saline Wipe clean. Those who are sensitive to pain can use anesthetics to apply on the face (but anesthetics can cause allergies, burn the skin, and affect skin metabolism), and then perform composition care after the facial acne is treated.
[0060] Use a roller needle to gently walk the empty needle on the face, and then apply the composition evenly on the face (apply in different areas, do not apply at one time) and start rolling. The rolled areas are divided into: forehead, cheeks, and chin. The direction of the needle rolling is in the shape of a rice, that is, horizontal, vertical and diagonal. 4-5 times in each direction, with moderate or mild strength...
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