Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Peptoid targeting Abeta42 fibrosome as well as preparation method and application thereof

A type and response technology, applied in the field of biomedicine, can solve the problems of high damage, lack of early detection methods and high cost of cerebrospinal fluid examination, and achieve the effect of strong sensitivity, high affinity and high accuracy

Pending Publication Date: 2022-01-14
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cerebrospinal fluid (CSF) and PET imaging examinations are expensive and harmful, and are difficult to be popularized by the general population
At present, Alzheimer's disease can only be diagnosed when the clinical symptoms are more obvious, and there is a lack of effective early detection methods and biochemical detection methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peptoid targeting Abeta42 fibrosome as well as preparation method and application thereof
  • Peptoid targeting Abeta42 fibrosome as well as preparation method and application thereof
  • Peptoid targeting Abeta42 fibrosome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 The peptoids of the present invention are synthesized by solid-phase subunit synthesis

[0053] This experimental example provides a peptoid of the present invention and its synthesis method.

[0054] The specific method is as follows:

[0055] 1. Resin swelling: Take 1 g of Rink Amide resin and place it in a synthesis tube, add excess DMF and shake it at room temperature for 1 hour, add 20% hexahydropyridine solution for deprotection, and wash with DCM and DMF alternately for 3 times.

[0056] 2. Coupling of cysteine ​​(cys): Weigh 10 times excess Cys according to the amount of resin, then mix it with HBTU in an equimolar ratio and dissolve it in 0.4mol / L NMM DMF solution. After fully dissolving Put it into a synthesis tube filled with resin, place it on a shaker and shake it for 1 hour, add 20% hexahydropyridine solution for deprotection, and wash with DCM and DMF alternately for 3 times.

[0057] 3. Subsequent subunit connection: According to the amount o...

Embodiment 2A

[0070] Example 2 Preparation of Aβ42 oligomers and fibrils

[0071] In this example, Aβ42 oligomers and fibrils were prepared. Specific steps are as follows:

[0072] (1) Monomerization treatment: In a fume hood, dissolve 2mg of Aβ42 monomer powder (purchased from Jill Biochemical (Shanghai) Co., Ltd.) in 1ml of cooled HFIP (hexafluoroisopropanol), sonicate for 30min, and incubate at room temperature After 2 hours, the Aβ42 was fully dissolved and placed in a fume hood for volatilization. After air-drying, a transparent Aβ peptide film is formed and stored in a -20°C refrigerator until use;

[0073] (2) Preparation of oligomers: the monomerized Aβ42 was added with 1% DMSO (dimethyl sulfoxide) to help dissolve it, and dissolved in PBS solution. Then centrifuge at 12000rpm at 4°C for 30min, and take the supernatant. current use;

[0074] (3) Fibrous body preparation: Add 1% DMSO (dimethyl sulfoxide) to aid dissolution of the monomerized Aβ42, dissolve it in PBS solution, pl...

Embodiment 3

[0077] Example 3 Binding ability test between peptides and β-amyloid polypeptide Aβ42 oligomers and fibrils

[0078] In this example, the peptoids prepared in Example 1 and the binding ability of Aβ42 oligomers and fibrils prepared in Example 2 were tested.

[0079] Specific steps are as follows:

[0080] 1. Preparation of the peptoid chip: pipette the peptoid solution prepared in Example 1 and drop it on the designated area of ​​the chip for SPRi detection. The volume of each spot solution is 0.3ul, and three spots are repeated for each sample.

[0081] 2. Incubation: Place the printed chip in a wet box and incubate overnight in a refrigerator at 4°C;

[0082] 3. Cleaning: Wash the incubated chip with 10×PBS for 10 minutes, 1×PBS for 10 minutes, and deionized water twice for 10 minutes;

[0083] 4. Sealing: The chip is completely submerged in 5% skimmed milk solution (diluted in PBST), and sealed in a refrigerator at 4° C. for more than 6 hours. Then repeat the previous cl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
affinityaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biomedicine, and particularly discloses a peptoid targeting Abeta42 fibrosome as well as a preparation method and application thereof. The peptoid comprises a first subunit: cysteine, a second subunit: 1,4-butanediamine, a third subunit: isobutylamine, a fourth subunit: ethanolamine, a fifth subunit: furfuryl amine, a sixth subunit: isobutylamine and a seventh subunit: 1, 4-butanediamine. The peptoid provided by the invention can be combined with a surface plasmon resonance imaging technology to detect Alzheimer's disease or amnesia mild cognitive impairment, and has the advantages of high specificity and strong sensitivity. In addition, the content of Abeta42 fibrosome in blood can be noninvasively and directly detected, the AD patients and normal people can be effectively distinguished by identifying AD marker amyloid protein in serum, and a new liquid biopsy method and thought are provided for diagnosing and monitoring the progress of the Alzheimer's disease or the amnesia mild cognitive impairment and medicine research and development.

Description

technical field [0001] The present invention relates to the field of biomedical technology, in particular to a peptide targeting Aβ42 fibrils and its preparation method and application. Background technique [0002] Alzheimer's disease (AD) is one of the most common age-related neurodegenerative diseases, and its clinical manifestations include progressive memory impairment, cognitive dysfunction and language impairment. At present, Alzheimer's disease patients account for 1% of the population over 60 years old, and the incidence rate doubles every five years. The number of people is large, which brings huge challenges to both patients and society. AD, as a common, frequently-occurring and high-burden disease of the elderly, has become one of the most concerned areas of scientists around the world. [0003] The lesion sites of different amyloid diseases are different, mainly involving the nervous system, heart, liver, kidney, etc. Some protein monomers are non-toxic or ver...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/04C07K1/06A61K38/08A61P25/28G01N33/68
CPCC07K7/06A61P25/28G01N33/6896A61K38/00
Inventor 胡志远王子华孙梦琦
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products