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Pharmaceutical composition for preventing and/or treating inflammatory storm and preparation and application thereof

A technology of composition and medicine, which is applied in the field of medicine, can solve the problems that the active ingredient of baicalin flavonoids has not been reported, etc.

Pending Publication Date: 2022-01-14
BEIJING WEHAND BIO PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of the active ingredients of Scutellaria baicalensis in the preparation of drugs for preventing and treating inflammatory storms.

Method used

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  • Pharmaceutical composition for preventing and/or treating inflammatory storm and preparation and application thereof
  • Pharmaceutical composition for preventing and/or treating inflammatory storm and preparation and application thereof
  • Pharmaceutical composition for preventing and/or treating inflammatory storm and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the preparation of drug phospholipid complex

[0055] According to the ratio of drug to lipid (1:3.5, w / w), weigh 1 g of the pharmaceutical composition (the mass ratios of baicalein, wogonin, and scutellarin A are respectively selected as 0.5:7:1 (ratio 1), 1.5:5:1 (ratio 2), 0.29:0.86:1 (ratio 3) and 0.43:0.57:1 (ratio 4) 3.5g of phospholipid S75, put in a rotary evaporator, add 20mL THF After the drug and phospholipid are completely dissolved, let it stand for 30 minutes, place it on a rotary evaporator, evaporate the solvent at 40°C, dry it under reduced pressure and vacuum for more than 12 hours, and scrape off the solid to obtain drug-phospholipid complexes with different drug ratios.

Embodiment 2

[0056] Embodiment 2, the preparation of self-microemulsion

[0057] Take ethyl oleate, Tween 80 and Transcutol HP by the mass ratio of 2:5:3, shake evenly, make blank self-microemulsion solution, add the medicine phospholipid complex prepared in embodiment 1 wherein, charging amount is 10% (taking the mass of blank milk as 100% dosage), put it in an air bath shaker, set the temperature at 25°C, and the rotation speed at 210 rpm, shake and dissolve completely, and then get self-microemulsions with different drug ratios.

Embodiment 3

[0058] Embodiment 3, the pharmacodynamic experiment of pharmaceutical composition and self-microemulsion

[0059] (1) Experimental materials

[0060] Baicalein API (purity 98%, Nanjing Zelang Biotechnology Co., Ltd., batch number: ZLS1903601);

[0061] Wogonin API (batch number 111514-201706, China National Institutes for Food and Drug Control), Melaleucain A API (batch number PRF10030442, Chengdu Pufarui Technology Development Co., Ltd.), Lipopolysaccharides from Escherichia coli 055:B5 (SIGMA); chlorine Sodium chloride injection (Sichuan Kelun Pharmaceutical Co., Ltd.); Mouse IL-6 ELISA Kit (Elabscience); Mouse Inflammation Panel, Mouse Cytokine Release Syndrome Panel, Mouse Proinflammatory Chemokine Panel (Biolegend); C57 BL / 6 male mice, 18 -22g, (Beijing Huafukang Biotechnology Co., Ltd.); XS 105DU 1 / 100,000 electronic balance (METTLER TOLEDO); 1-14 centrifuge (SIGMA); VORTEX-2 GENIE vortex instrument (Scientific Industries ); Flow cytometer C6 (BD Accuri).

[0062] (2)...

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PUM

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Abstract

The invention discloses a pharmaceutical composition for preventing and / or treating inflammatory storm, a preparation and application thereof. The pharmaceutical composition is prepared from baicalein, wogonin and oroxylin A, and the mass ratio of the baicalein to the wogonin to the oroxylin A is (0.25-1.5):(0.5-7):1. The self-microemulsion provided by the invention comprises the following components: a drug phospholipid complex, an oil phase, an emulsifier and a co-emulsifier, wherein the drug phospholipid complex is formed by compounding the medicine composition and a phospholipid material. Experimental results show that in an LPS-induced systemic inflammation storm mouse model, the composition of the baicalein, the wogonin and the oroxylin A and the self-microemulsion preparation of the composition have the effects of inhibiting mouse cytokine storm to different degrees. The pharmaceutical composition for preventing and / or treating inflammatory storm, the preparation and the application thereof provide a safe, effective and economical solution for clinical treatment of inflammatory storm.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a pharmaceutical composition for preventing and / or treating inflammatory storm, its preparation and application. Background technique [0002] Inflammatory storm is an excessive immune response and inflammatory response of the body to external stimuli, characterized by the excessive proliferation of T cells and macrophages and the rapid release and overexpression of inflammatory cytokines. Inflammatory storms can be caused by many infectious or noninfectious diseases, such as influenza, new coronary pneumonia (COVID-19), Middle East respiratory syndrome (MERS-CoV), atypical pneumonia (SARS), familial hemophagocytic syndrome, sepsis, systemic Juvenile idiopathic arthritis (sJIA), chimeric antigen receptor (CAR)-T cell therapy and lupus erythematosus, etc. [0003] The inflammatory storm phenomenon caused by respiratory virus infection such as influenza virus and coronavirus is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K9/107A61K47/24A61P1/00A61P1/16A61P1/18A61P19/02A61P29/00A61P31/04A61P31/12A61P31/14A61P31/16A61P37/02
CPCA61K9/1075A61K31/352A61K47/24A61P29/00A61P31/14A61P31/16A61P31/04A61P31/12A61P1/16A61P19/02A61P1/00A61P1/18A61P37/02A61K2300/00
Inventor 刘志华刘玉玲刘璐冯遇姜丹刘瑾如王丽叶军邹媛媛
Owner BEIJING WEHAND BIO PHARMA CO LTD
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