Kit for detecting catecholamine and intermediate metabolites thereof in human urine and test method

Abstract

The invention provides a kit for detecting catecholamine and intermediate metabolites thereof in human urine, and the kit comprises the following reagents: (1) a calibrator solution, which is divided into calibrators of six concentrations, the calibrator of each concentration contains six catecholamines and metabolites thereof: epinephrine, noradrenaline, dopamine, methoxyepinephrine, methoxynoradrenaline and 3-methoxytyramine, and the concentration range of the calibrator is 1ng / mL to 3000ng / mL; (2) quality control products, which include a quality control product QCL, a quality control product QCM and a quality control product QCH; (3) an internal standard solution, namely a methanol solution mixed solution containing six catecholamines and metabolites thereof; (4) a buffer solution I; (5) a buffer solution II; and (6) a derivative solution. The sample is treated through derivatization reaction, the problem that catecholamine and intermediate metabolites thereof in the sample are easy to degrade is solved, and the sample is stable in room temperature state after derivatization.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a kit and a test method for detecting human urine catecholamines and their intermediate metabolites. Background technique [0002] Catecholamine (CA), also known as catecholamine, mainly includes epinephrine (Epinephrine, referred to as E), norepinephrine (Norepinephrine, referred to as NE) and dopamine (Dopamine, referred to as DA). Catecholamine is a monoamine neurotransmitter synthesized and secreted by the adrenal medulla, adrenal neurons, and extra-adrenal chromaffin bodies. It is an endogenous substance with strong physiological activity and plays an important role in brain and nerve signal transduction. It not only plays an extensive role in regulating the physiological activities of the human body's cardiovascular system, nervous system, endocrine system, kidney, smooth muscle tissue, etc., but also affects the body's metabolism at the same time. The inter...

AI Technical Summary

Problems solved by technology

[0005] The detection of catecholamines and metabolites has gone through a variety of methods, and these traditional methods have their inherent shortcomings: bioanalysis, colorimetry, low sensitivity; fluorescence method, easily interfered by other endogenous compounds; radioenzyme method, The operation is cumbersome; the detection sensitivity of the chemiluminescence method cannot meet the needs of clinical testing; while the liquid chromatography coupled with electrochemistry (LC-ECD) has high sensitivity and selectivity, but it takes a long time and is susceptible to interference from various substances

MN and NMN have high specificity and sensitivity, so they are recommended by the Endocrinology Branch of the Chinese Medical Association as specific markers for the diagnosis of pheochromocytoma and paraganglioma; 2. The detection range is narrow, such as the linearity of detecting adrenaline in patent CN 111398448 The range is 0.5-50ng / mL, while the linear range of metanephrine and norepinephrine is 1-100ng / mL; 3. The sample pretreatment is complicated and the preparation cost is high

For example, the method provided by the patent CN109709255 requires such necessary steps as solid phase extraction and nitrogen blowing in the sample preparation process, thereby increasing the time and cumbersome steps of sample pretreatment

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