Method for separating cellulose from bagasse and performing enzymolysis

A sugarcane bagasse and cellulose technology, used in fiber raw materials, fiber raw material processing, papermaking, etc., can solve the problems of general removal of lignin and hemicellulose, improve the enzymatic sugar production efficiency and enzymatic hydrolysis rate, and improve Enzymatic hydrolysis efficiency and mild reaction conditions

Pending Publication Date: 2022-01-11
SOUTH CHINA AGRI UNIV
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the removal effect of this treatment on lignin and hemicellulose is average, and needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] S1. Add 60% (v / v) ethanol aqueous solution to the bagasse raw material according to the ratio of absolute dry mass volume ratio 1g: 10mL, and then add 0.005mol / L ethanol aqueous solution of CuCl 2 , react in a high-pressure reactor at 160°C. After the reaction time reaches 10 minutes, stop heating, and immediately use condensed water to lower the reaction to room temperature, and use vacuum filtration to separate the pretreatment residue.

[0068] The component analysis of the pretreatment residue shows that the removal rates of hemicellulose and lignin can reach 26.5% and 35.4%, respectively, and the retention rate of cellulose can reach 99.6%.

[0069] S2. Take 2 grams (on an absolute dry basis) of the pretreatment residue, add 20 FPU of cellulase and 100 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 72 hours of ...

Embodiment 2

[0072] S1. Add 60% (v / v) ethanol aqueous solution to the bagasse raw material according to the ratio of absolute dry mass volume ratio of 1g: 10mL, and then add 0.01mol / L ethanol aqueous solution of CuCl 2 , react in a high-pressure reactor at 160°C. After the reaction time reaches 10 minutes, stop heating, and immediately use condensed water to lower the reaction to room temperature, and use vacuum filtration to separate the pretreatment residue.

[0073] The component analysis of the pretreatment residue shows that the removal rates of hemicellulose and lignin can reach 50.8% and 45.2%, respectively, and the retention rate of cellulose can reach 98.9%.

[0074] S2. Take 2 grams (on an absolute dry basis) of the pretreatment residue, add 20 FPU of cellulase and 100 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 72 hours o...

Embodiment 3

[0077] S1. Add 60% (v / v) ethanol aqueous solution to the bagasse raw material according to the ratio of absolute dry mass volume ratio 1g: 10mL, and then add 0.025mol / L ethanol aqueous solution of CuCl 2 , react in a high-pressure reactor at 160°C. After the reaction time reaches 10 minutes, stop heating, and immediately use condensed water to lower the reaction to room temperature, and use vacuum filtration to separate the pretreatment residue.

[0078] The component analysis of the pretreatment residue shows that the removal rates of hemicellulose and lignin can reach 83.2% and 71.4%, respectively, and the retention rate of cellulose can reach 95.0%.

[0079] S2. Take 2 grams (on an absolute dry basis) of the pretreatment residue, add 20 FPU of cellulase and 100 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 72 hours of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for separating cellulose from bagasse and performing enzymolysis. The method comprises the following steps: uniformly mixing bagasse, an ethanol water solution and copper chloride, performing heat preservation treatment at 130-180 DEG C, and separating to obtain a pretreatment solution and the cellulose; and adding a buffer solution and cellulase into the cellulose, and carrying out enzymolysis to obtain a glucose liquid. According to the invention, the cellulose separation condition is mild, most hemicelluloses and part of lignin in the bagasse can be effectively removed, cellulose is reserved, and high-quality cellulose can be obtained; and in the prepared cellulose, the contents of hemicellulose and lignin are low, the influence of hemicellulose and lignin on enzymolysis is reduced, the enzymolysis efficiency is preliminarily improved, and the cellulose can be more favorably used as a raw material for cellulase enzymolysis.

Description

technical field [0001] The invention relates to the technical field of high-efficiency conversion and utilization of biomass, in particular to a method for separating cellulose from bagasse by catalyzing ethanol pretreatment with copper chloride, and also relates to a method for further enzymatically decomposing cellulose into glucose. Background technique [0002] With the reduction of fossil energy, the pollution of the environment and the enhancement of environmental awareness, people are forced to look for a new clean energy. As one of them, biomass energy has attracted people's attention. Among the abundant biomass resources, lignocellulosic biomass has many kinds and is easy to obtain. The production of liquid fuel from lignocellulosic biomass is not only a resource utilization of waste, but also has great significance to my country's energy security, and has attracted people's attention. However, since the cellulose in lignin-based biomass is closely connected with ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): D21C5/00D21B1/02C12P19/02C12P19/14
CPCD21C5/00D21B1/021C12P19/02C12P19/14
Inventor 张红丹张贤滨谢君陆勇健
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap