Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof

A technology for inhibiting peptides and low bitterness, applied in the field of deep processing of agricultural and sideline products, can solve problems such as severe bitterness, and achieve the effects of easy digestion and absorption, promoting sustainable development, and small molecular weight

Pending Publication Date: 2022-01-11
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ACE inhibitory peptides often produce severe bitter taste due to the exposure of hydrophobic groups during the enzymatic preparation process, which limits their application in food, health care products and other fields.

Method used

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  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof
  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof
  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing low-bitter ACE inhibitory peptides from defatted sesame powder, which specifically includes the following steps:

[0035] 1) Preparation of defatted sesame powder:

[0036] The sesame cake was crushed and degreased using a Soxhlet extractor. The solvent was petroleum ether (boiling point 40-60°C), the extraction temperature was 60°C, and the degreasing was performed for 7 h. Grind the defatted sesame meal raw material, pass through a 100-mesh sieve, and collect the under-sieve, which is defatted sesame powder. The protein content determined by Kjeldahl method (GB5009.5-2003) is 51.23%;

[0037] 2) Proteolysis:

[0038] The defatted sesame powder obtained in step 1) is added with distilled water according to the material-to-liquid ratio of 10 g: 100 mL and stirred evenly. Use 6 mol / L NaOH to adjust the pH to 8.2, then add 9000 U / g at 50-55°C 原料蛋白 Add 4000 U / g of alkaline protease Alcalase after enzymolysis at 50-55℃ for 1 h 原料蛋白 Flavor protease...

Embodiment 2

[0045] The invention discloses a method for artificially synthesizing low-bitterness sesame ACE inhibitory peptide, which is artificially synthesized by a solid-phase synthesis method. The basic process is as follows: first, an amino acid whose amino group is protected by Fmoc group is connected to the insoluble solid phase carrier Wang resin, and then the protecting group of the amino group is removed, and the first amino acid is connected to the solid phase carrier; secondly, the amino acid group is protected by Fmoc group. The carboxyl group of the second amino acid protected by the group is activated with a condensing agent, and the activated amino acid reacts with the amino group of the first amino acid that has been attached to the solid phase carrier to form a peptide bond. At this time, a Dipeptides with protective groups. Repeat the above-mentioned peptide bond formation reaction to grow the peptide chain from the C-terminus to the N-terminus until the required length...

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Abstract

The invention belongs to the technical field of deep processing of agricultural and sideline products, and particularly relates to a low-bitterness sesame ACE (angiotensin converting enzyme) inhibitory peptide which consists of four amino acids and has the sequence of Trp-Leu-Tyr-Arg (WLYR). The low-bitterness sesame polypeptide can be obtained through proteolysis of degreased sesame powder, debitterizing, separation and purification, and can also be artificially synthesized through adoption of a chemical solid-phase synthesis method. Sensory evaluation, electronic tongue analysis and ACE inhibition capability determination show that the polypeptide basically has no bitter taste, has strong antihypertensive activity, belongs to food-borne antihypertensive peptides, has small molecular weight, is easy to absorb, can be used as a functional active component for preparing antihypertensive drugs, and can be used as active drugs in the fields of health care products, food, medicines and the like, snd the market prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural and sideline products, and specifically relates to a low-bitter ACE inhibitory peptide WLYR derived from defatted sesame powder, a preparation method and its application in blood pressure-lowering drugs, health products, food, etc. Background technique [0002] Hypertension is the main cause of coronary heart disease, arteriosclerosis, stroke, and heart failure. According to epidemiological studies, more than 1 billion people now suffer from high blood pressure. Therefore, the prevention and treatment of hypertension has become a global public problem to be solved urgently. Angiotensin I transferase (ACE) is a dipeptide carboxypeptidase that plays an important role in blood pressure regulation. At present, the drugs for the treatment of hypertension mainly include sartan and dipine, but these chemically synthesized drugs often cause side effects such as cough, rash, and h...

Claims

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Application Information

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IPC IPC(8): C07K5/117C12P21/06C07K1/34C07K1/16C07K1/14
CPCC07K5/1024C12P21/06
Inventor 贾聪芦鑫孙强黄纪念
Owner HENAN ACAD OF AGRI SCI
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