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Application of XPR1 inhibitor in preparation of product for inhibiting migration and/or proliferation of thyroid cancer cells

A technology for thyroid cancer and cell migration, which is applied in the field of tumor molecular biology to achieve the effect of reducing the ability of migration and proliferation

Active Publication Date: 2021-12-17
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of XPR1 in human malignancies, especially in thyroid cancer, remains to be elucidated

Method used

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  • Application of XPR1 inhibitor in preparation of product for inhibiting migration and/or proliferation of thyroid cancer cells
  • Application of XPR1 inhibitor in preparation of product for inhibiting migration and/or proliferation of thyroid cancer cells
  • Application of XPR1 inhibitor in preparation of product for inhibiting migration and/or proliferation of thyroid cancer cells

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Experimental program
Comparison scheme
Effect test

Embodiment

[0061] 1. Materials and methods

[0062] 1.1 Bioinformatics analysis

[0063] Thyroid cancer patient information with complete clinical case characteristics (age, gender, LNM, tumor size, clinical stage, etc.) was downloaded from the TCGA database for analysis. GSEA software (GSEA v3.0, http: / / www.broadinstitute.org / gsea) was used to perform single gene enrichment analysis between the low XPR1 and high XPR1 expression groups of the TCGA database.

[0064] 1.2 RNA extraction, reverse transcription and quantitative real-time fluorescent polymerase chain reaction (qRT-PCR)

[0065] 1.2.1 RNA extraction

[0066] 1.2.1.1 Tissue specimens: Take them out from the frozen tube, put them into a 1.5 ml EP tube, and add 1 ml Trizol lysate. Afterwards, it was fully lysed with a tissue homogenizer, and then kept on ice. Cell samples: Discard the plate supernatant, add 1 ml PBS to wash once, absorb as much waste liquid as possible, add 1 ml Trizol lysate, let it stand for 5 min, then pip...

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Abstract

The invention belongs to the field of tumor molecular biology and particularly relates to application of an XPR1 inhibitor in preparation of a product for inhibiting migration and / or proliferation of thyroid cancer cells. The invention finds that the expression of an XPR1 gene in the thyroid cancer cells is obviously higher than that of normal thyroid cells, and the influence of the XPR1 gene on the migration and proliferation capacity of the thyroid cancer cells is verified through a CCK-8 experiment, a colony formation experiment, a Transwell migration experiment, a Matrigel invasion experiment, a flow cytometry, a cell apoptosis experiment and a Western blot experiment. The invention finds that the migration and proliferation capacity of the thyroid cancer cells can be effectively lowered by knocking down the expression of the XPR1 gene.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of XPR1 inhibitors in the preparation of products for inhibiting the migration and / or proliferation of thyroid cancer cells. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Biomarkers refer to biochemical indicators that can mark changes or possible changes in system, organ, tissue, cell and subcellular structure or function, and have a very wide range of uses. Biomarkers can be used for disease diagnosis, tumor burden monitoring, risk assessment for judging tumor recurrence and metastasis, or for evaluating the safety and effectiveness of new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61P35/00A61P35/04
CPCA61K31/7088A61P35/00A61P35/04
Inventor 陈恩东尤捷陈不染陈盈盈李权胡孝渠项晓蔡业丰王青煊杜远
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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