Application of atractylenolide III in preparation of anti-hepatic fibrosis drugs

An anti-hepatic fibrosis, Atractylodes lactone technology, applied in the application field of Atractylodes lactone III in the preparation of anti-hepatic fibrosis drugs

Pending Publication Date: 2021-12-17
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The anti-hepatic fibrosis activity of atractyloid III has not been reported yet

Method used

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  • Application of atractylenolide III in preparation of anti-hepatic fibrosis drugs
  • Application of atractylenolide III in preparation of anti-hepatic fibrosis drugs
  • Application of atractylenolide III in preparation of anti-hepatic fibrosis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Observation of the effects of Atractylolide III on liver tissue damage and liver fibrosis indicators in mice.

[0019] 48 male ICR mice were purchased from Shanghai Slack Experimental Animal Co., Ltd., license number: SCXK (Shanghai) 2020-0025. Animals were given free access to food and water, and were given standard pellet feed. The experiment began after one week of adaptive feeding in a standard light cycle (12 hours of light, 12 hours of darkness), room temperature 22°C, and constant humidity. carbon tetrachloride (CCl 4 ; Analytical grade, Nanjing Chemical Reagent Co., Ltd.) and olive oil (Shandong Luhua Group Co., Ltd.) made 10% olive oil preparation at a volume ratio of 1:9. Atractylodes lactone III was purchased from Chengdu Zhibiao Pure Biotechnology Co., Ltd. (batch number: PCS0114, with a purity of over 99%, the same below), and was prepared into a solution with olive oil. Animals were randomly divided into six groups: normal group, model group, ...

Embodiment 2

[0030] Example 2: The mouse liver tissue of Example 1, the liver morphology was photographed, the liver tissue was fixed with 4% paraformaldehyde, embedded, and sectioned for HE staining, Sirius red staining and Masson staining, to observe liver fibrosis, liver pathology and collagen deposition Impact.

[0031] HE staining of the liver sections of mice in the model group showed degeneration and necrosis of hepatocytes, showing a large area of ​​pseudolobular tissue structure; Masson staining showed a large amount of blue collagen fiber deposition, extending outward from the portal area, and the fiber cords were thicker and more stained. Deep, indicating that there are many collagen fibers; Sirius red staining indicates that various types of collagen fibers expand outward from the portal area, and observed under a polarizing microscope, collagen deposition and expansion have formed pseudolobules, among which a large number of red type I collagen fibers, a small amount of sparse ...

Embodiment 3

[0034] Example 3: HSCs were cultured in vitro, and the inhibitory effect of Atractylolide III on HSC proliferation was observed (detection of cell proliferation activity).

[0035] HSC-LX2 in the logarithmic growth phase was inoculated in a 96-well plate, and the concentration of the cell suspension was adjusted to 1×10 per well. 4 cells in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2Cultivate for 24 hours under culture conditions to make the cells adhere to the wall. The cells were divided into 10 groups. Except the normal control group, the cells in the other 9 groups were treated with atractyloid III (1, 2, 5, 10, 20, 30, 40, 50, 100 μM) for 24 hours. Each group has 6 replicate wells. After the effect was over, 20 μl of a 5% concentration of thiazolium blue (MTT) solution was added to each well, and the incubation was continued for 4 hours. Then the supernatant was sucked off, 200 μl DMSO was added to each well, and shaken on a shaker at low speed for 1...

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Abstract

The invention particularly relates to an application of atractylenolide III in preparation of an anti-hepatic fibrosis drug. The application of the atractylenolide III in preparation of the anti-hepatic fibrosis drug researches find that the atractylenolide III reduces the contents of ALT, AST and ALP in serum of a mouse with hepatic fibrosis, reduces the contents of fibrosis indexes HA, LN and PCIII in the serum of the mouse with hepatic fibrosis, can inhibit expression of HSC activation indexes alpha-SMA and COL1 alpha 1 in a dose-dependent manner. Therefore, the atractylenolide III can be used for preparing the anti-hepatic fibrosis drug.

Description

technical field [0001] The invention relates to a chemical component-Atractylenolide III, in particular to the application of Atractylenolide III in the preparation of anti-hepatic fibrosis drugs. Background technique [0002] Liver fibrosis is a common pathological process in the development of various chronic liver diseases. Various reasons lead to excessive proliferation and abnormal deposition of extracellular matrix components in liver tissue, which in turn causes abnormal changes in liver structure or function. Dysfunction and failure seriously endanger life and health. Delaying or even reversing liver fibrosis is of great significance to reducing the occurrence of serious complications and improving the quality of life of patients. [0003] The essence of liver fibrosis is that the synthesis of extracellular matrix (ECM) in the liver tissue is greater than the degradation, resulting in the excessive deposition of a large amount of ECM in the liver. Hepatic stellate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61K36/284A61P1/16A61K125/00
CPCA61K31/365A61K36/284A61P1/16
Inventor 郑仕中张峰王飞虾陈利李章昊杨婷邵江娟张自力连娜琦
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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