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Application of Oleaceran in liver fibroblast proliferation inhibiting drugs

A technology of fibroblasts and drugs, applied in the direction of drug combinations, pharmaceutical formulations, organic active ingredients, etc., can solve the problems of hepatic stellate cell apoptosis and deficiency

Inactive Publication Date: 2015-04-08
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ideal clinical treatment methods are still lacking. At present, the key to preventing and treating liver fibrosis is to target the links related to the activation of hepatic stellate cells, mainly: reducing liver damage; inhibiting the activation of stellate cells and reducing the production of extracellular matrix; regulating cytokine disorders , promote apoptosis of activated hepatic stellate cells

Method used

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  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs
  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs
  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Preparation of the compound Oleaceran tablet of the present invention:

[0011] Take 5 grams of compound Oleaceran, add 195 grams of dextrin, mix well, and make 1000 tablets by conventional tableting.

Embodiment 2

[0012] Example 2: Preparation of the compound Oleaceran capsule of the present invention:

[0013] Take 5 grams of compound Oleaceran, add 195 grams of starch, mix well, and put into capsules to make 1000 capsules.

[0014] The pharmacodynamics experiment will further illustrate its drug activity below.

experiment example 1

[0015] Experimental example 1: MTT method was used to evaluate the inhibitory effect of compound Oleaceran on liver fibrosis

[0016] 1. Methods: The NIH / 3T3 cell line was purchased from the cell bank of the Shanghai Institute of Cell Research, Chinese Academy of Sciences, which was quoted from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made into 1 × 10 cells in DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, trypan blue staining to identify the viability of more than 95%, add 100ul per well to a 96-well plate, at 37°C, 5% CO 2 After 24 hours of synchronous treatment, the supernatant was discarded, 200 ul of DMEM medium (containing 10% FCS) containing drugs at different dilution concentrations was added, and 48 hours of culture were added, and MTT solution was added to each well to incubate for 4 hours. Discard the culture medium, add 150ul DMSO, shake ...

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Abstract

The invention discloses an application of Oleaceran in preparation of liver fibrosis treatment drugs. Pharmacological experiment proves that the Oleaceran can obviously inhibit the occurrence and development of liver fibrosis and can be used for preparing drugs resisting liver fibrosis and preventing liver cirrhosis; the Oleaceran belongs to a brand new skeleton type, has strong inhibitory activity on the liver fibroblast proliferation and has outstanding substantive features; meanwhile, a remarkable progress in obviously realized by applying the Oleaceran to resisting liver fibroblast proliferation.

Description

technical field [0001] The invention relates to a new use of the compound Oleaceran, in particular to the application of Oleaceran in the preparation of a drug for inhibiting the proliferation of liver fibroblasts. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis. In the past 20 years, the research on liver fibrosis has made great progress, and it has been confirmed that liver fibrosis and a certain degree of liver cirrhosis are reversible. However, ideal clinical treatment methods are still lacking. At present, the key to preventing and treating liver fibrosis is to target the links related to the activation of hepatic stellate cells, mainly: alleviating liver damage; inhibiting stellate cell activation and reducing extracellular matrix production; regulating cytokine disorders , promotes apoptosis of activated hepatic stellate cells. [0003] The compound Oleaceran involved in the present invention was publishe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/343A61P1/16
Inventor 吴玉龙程梅王健赵冬梅倪天辉周秀芝顾伟于娟
Owner BINZHOU MEDICAL COLLEGE
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