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Pentoxyverine citrate hapten, antigen, antibody, detection device and preparation method

A technology of pentoxaverine citrate and hapten, which is applied in the field of antibodies and detection devices and preparations, and the field of pentoxaverine citrate hapten and antigen, can solve the problem that the testing personnel have high operational technical requirements and cannot meet the requirements of rapid detection. , high requirements for testing instruments, to achieve the effect of economical, fast, easy to manufacture and low cost

Active Publication Date: 2022-02-18
广东江门中医药职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above detection methods have high requirements for detection instruments, the instruments are expensive, difficult to popularize, and the detection time is long, the efficiency is low, and the detection personnel have high requirements for operating skills, which cannot meet the needs of on-site rapid detection.

Method used

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  • Pentoxyverine citrate hapten, antigen, antibody, detection device and preparation method
  • Pentoxyverine citrate hapten, antigen, antibody, detection device and preparation method
  • Pentoxyverine citrate hapten, antigen, antibody, detection device and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of pentoxyverine citrate hapten:

[0027] S1, add 2.0g α-phenylcyclopentylacetic acid and 30ml thionyl chloride successively to a 100ml single-necked bottle, stir until reflux, reflux reaction for 3-4h, TLC monitors that the reaction material α-phenylcyclopentylacetic acid disappears, drops Stir at room temperature, distill the reaction solution to dryness under reduced pressure at 50°C, add dichloromethane to 10ml, stir until the solution is clear, and prepare α-phenylcyclopentylacetyl chloride for use;

[0028] S2, add 3.0g of diethylene glycol acetal and 5ml of dichloromethane to another 100ml one-necked bottle successively, stir at room temperature, after mixing evenly, turn to stirring under ice bath, drop the above-mentioned α-phenylcyclopentylacetyl chloride into Wherein, after dropping, turn to room temperature and stir for 1 h, then raise the bath temperature to 40° C. and stir for 5 h, then lower to room temperature and stir, add 20 ml o...

Embodiment 2

[0031] The preparation method of pentoxyverine citrate immune antigen:

[0032]Take 0.1 mmol of the pentoxyverine citrate hapten prepared in Example 1 and dissolve it in 2 ml of dimethylformamide (DMF), stir and add 0.2 mmol of dicyclohexylcarbodiimide (DCC) and 0.1 mmol of N- Hydroxysuccinimide (NHS), reacted overnight with magnetic stirring at 4°C, centrifuged and took the supernatant to prepare liquid A; weigh 140mg of bovine serum albumin (BSA) and dissolve it in 10ml with a concentration of 0.1mol / L, pH Add 1ml of DMF solution to the PBS solution with a value of 8.0, stir and dissolve to prepare liquid B; under magnetic stirring, drop liquid A into liquid B drop by drop, react at 4°C for 12h, after centrifugation, take the supernatant, and put it in Dialyze with normal saline at 4°C for 3 days, change the dialysate 3 times a day, and prepare the pentoxyverine citrate immune antigen, that is, the pentoxyverine citrate-BSA antigen. The BSA antigen was dispensed into 0.5mL ...

Embodiment 3

[0034] The preparation method of pentovir citrate antigen for detection line:

[0035] Take 0.1mmol of the pentoxyverine citrate hapten prepared in Example 1 and dissolve it in 2ml of DMF, add 0.2mmol of DCC and 0.1mmol of NHS with stirring, react overnight with magnetic stirring at 4°C, centrifuge the supernatant overnight, and prepare Obtain liquid A; weigh 120 mg of chicken ovalbumin (OVA) and dissolve it in 10 ml of PBS solution with a concentration of 0.1 mol / L and a pH value of 8.0, add 1 ml of DMF solution, stir and dissolve to prepare liquid B; under magnetic stirring, dissolve liquid A Drop into solution B drop by drop, react at 4°C for 12h, centrifuge, take the supernatant, dialyze with normal saline at 4°C for 3 days, change the dialysate 3 times a day, and use citrate penetrant to obtain the detection line Verin antigen, that is, pentoxyverine citrate-OVA antigen, the pentoxyverine citrate-OVA antigen is divided into 0.5ml centrifuge tubes at a concentration of 10m...

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Abstract

The invention discloses a pentoxaverine citrate hapten, an antigen, an antibody, a detection device and a preparation method, wherein the structure of the citrate pentoxaverine hapten is shown in (I). The invention adopts the immunodetection method to detect the illegally added pentoxaverine citrate in Chinese patent medicines, health products or traditional Chinese medicine drinks, and has the advantages of good specificity, high sensitivity, simple operation, and on-site rapid detection, etc. It improves the detection efficiency, achieves the purpose of rapid detection, and can better meet the needs of on-site supervision and law enforcement by regulatory departments and testing agencies. (I).

Description

technical field [0001] The invention relates to the field of drug safety detection, in particular to pentoxyverine citrate hapten, antigen, antibody, detection device and preparation method. Background technique [0002] Pentoxyverine citrate is used for dry cough without phlegm and pertussis caused by upper respiratory tract infection, and its curative effect on children is better than that on adults. Illegal addition of chemical drugs in Chinese patent medicines and health foods also occurs from time to time. In order to increase the curative effect and make huge profits, some unscrupulous merchants add chemical drugs that can promote curative effect to such products. These chemical drugs have not been verified by experiments. Verification and strict review and approval by relevant national departments are difficult to guarantee its quality and safety; at the same time, the types and dosages added are even more random and uncertain. This phenomenon is seriously harmful and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/753C07C69/616C07C67/08C07K14/765C07K14/77C07K14/795C07K1/107C07K16/44C07K16/06G01N33/577G01N33/558G01N33/548G01N33/58
CPCC07C69/753C07C69/616C07K14/765C07K14/77C07K14/795C07K16/44C07K16/06G01N33/577G01N33/558G01N33/548G01N33/587G01N33/94
Inventor 李拥军熊文明敬美莲温宝莹皮小弟李叶青程文海谭晓玉
Owner 广东江门中医药职业学院
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