Colloidal gold detection device mixed with spearmint in mint and its decoction pieces, preparation method and application

A detection device and colloidal gold technology, which are applied to measurement devices, biological tests, material inspection products, etc., can solve the problem of inability to quickly detect on-site, and achieve the effects of economy, speed, convenience and low cost.

Active Publication Date: 2021-11-05
SHANXI PROVINCE FOOD & DRUG INSPECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the technical problem that the current technology cannot detect quickly on site, the present invention provides a colloidal gold detection device that is easy to use, fast and simple, high in sensitivity and accuracy, and capable of on-site determination of carvone and its preparation method and use

Method used

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  • Colloidal gold detection device mixed with spearmint in mint and its decoction pieces, preparation method and application
  • Colloidal gold detection device mixed with spearmint in mint and its decoction pieces, preparation method and application
  • Colloidal gold detection device mixed with spearmint in mint and its decoction pieces, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of carvone hapten

[0040] Add 1.5g of carvone, 1.4g of carboxymethoxylamine hemihydrochloride, 8mL of methanol, 2mL of pyridine, and 2mL of distilled water into a 100mL three-necked flask, and react at 65°C for 5h. After rotary evaporation, add water, adjust the solution to slightly acidic with dilute hydrochloric acid, extract with ethyl acetate 2-3 times, combine the organic phases, evaporate to dryness, mix the samples, and purify by column to obtain the carvone hapten.

Embodiment 2

[0041] Example 2 Synthesis of Carvone Immune Antigen

[0042] Immunizing antigens were prepared using the carvone hapten. Take carvone hapten 0.1mmol dissolved in 2mL DMF, stir and add 0.2mmol DCC and 0.15mmol NHS. Magnetic stirring was carried out overnight at 4°C. After centrifugation, the supernatant was liquid A. Weighed 140 mg of hemocyanin (KLH) and dissolved it in 10 mL of PBS (pH 8.0) with a concentration of 0.1 mol / L. Add DMF1mL, stir and dissolve to prepare liquid B, under magnetic stirring, gradually drop liquid A into liquid B, and react at 4°C for 12h. After centrifugation, the supernatant was taken, dialyzed with normal saline at 4°C for 3 days, and the dialysate was changed 3 times a day. The obtained whole antigen was dispensed into 0.5 mL centrifuge tubes at a concentration of 1 mg / mL. Store frozen in a -20°C freezer.

Embodiment 3

[0043] Example 3 Preparation of Carvone Monoclonal Antibody

[0044] The monoclonal antibody was prepared by immunizing the antigen with carvone. Use carvone to immunize the antigen and immunize four 6-week-old BALB / C mice after identification. After boosting the immunization three times, blood is collected to measure the titer. When the serum titer no longer rises, the mouse is immunized with twice the dose of antigen without adjuvant. Mice were killed by dislocation of the neck three days later, the spleen was taken under aseptic conditions to prepare splenocytes, mixed with vigorously growing mouse myeloma cells in a 50mL centrifuge tube at a ratio of 8:1, and 30mL of serum-free IPMI1640 medium was added. Centrifuge at 1100r / min for 5min, discard the supernatant, loosen the cell mass, and place in a 37°C water bath. Slowly add 1 mL of 50% PEG-4000 to the cells, drop it within 1 min, and gently stir the sediment at the bottom. After standing for 1 min, slowly and uniformly ...

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Abstract

The patent of the present invention discloses a colloidal gold detection device and its preparation method, aiming to provide a fast, simple, high-sensitivity quick screening kit that can detect mint and its decoction pieces mixed with easy-to-mix spearmint, which is suitable for traditional Chinese medicine The invention relates to on-site determination of mixed products mixed with decoction pieces, which belongs to the technical field of biological detection. The device consists of a test strip and a reaction cup. Wherein the test strip includes a bottom plate, a sample pad laid in sequence along the same direction, a nitrocellulose membrane and an absorbent paper, the nitrocellulose membrane includes a detection line and a quality control line; the reaction cup contains a colloidal gold-labeled antibody. The present invention applies the principle of chromatographic immune colloidal gold, the detection line in the test paper and the quality control line are colorimetric to semi-quantitatively detect the residual carvone in the sample, and quickly and accurately detect whether the sample contains carvone in a short time. It can meet the needs of the supervision department of traditional Chinese medicine decoction pieces for the rapid detection of spearmint mixed with mint and its decoction pieces, and can meet the needs of supervision departments and testing institutions for on-site supervision and law enforcement.

Description

technical field [0001] The patent of the present invention discloses a colloidal gold detection device and its preparation method, aiming to provide a fast, simple, high-sensitivity device that can detect mint and its decoction pieces mixed with easy-to-mix spearmint (the characteristic component carvone). The sieving kit is suitable for on-the-spot determination of Chinese medicine decoction pieces mixed with easily mixed products, and belongs to the technical field of biological detection. Background technique [0002] Mint is the dry aerial part of Mentha haplocalyx Briq, which is a commonly used traditional Chinese medicine. From the daily inspection of medicinal materials and market research, it is found that the mint medicinal materials and decoction pieces have the problem of mixing or replacing spearmint, which belongs to the same plant. Both peppermint and spearmint are plants of the genus Mentha of the Labiatae family, and are aromatic plants, both of which are ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/52
CPCG01N33/52G01N33/577
Inventor 李民生崔宇宏郭景文杨国伟李琛张俊凤王爱那王玳
Owner SHANXI PROVINCE FOOD & DRUG INSPECTION INST
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