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Manufacturing of medical fulvic acid standard substance and application of medical fulvic acid standard substance in cell experiment

A technology of fulvic acid and standard products, which can be used in the detection of programmed cell death, drug screening, and compound screening. Effects of health, value-added enhancement, and economic prosperity

Pending Publication Date: 2021-12-03
上海臻衍生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2) Brain tumors grow infiltratively, cannot be completely eradicated, and easily recur
3) Brain tumors are not very sensitive to radiation, and the effect of radiotherapy is poor
4) The vast majority of anti-cancer chemotherapy drugs cannot enter the brain through the blood-brain barrier, so it is difficult to achieve good results

Method used

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  • Manufacturing of medical fulvic acid standard substance and application of medical fulvic acid standard substance in cell experiment
  • Manufacturing of medical fulvic acid standard substance and application of medical fulvic acid standard substance in cell experiment
  • Manufacturing of medical fulvic acid standard substance and application of medical fulvic acid standard substance in cell experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Get 100 parts of lignite raw materials, after screening and pulverizing, at 130° C. of reactor temperature, under reaction pressure 0.13 MPa, adopt concentration to be 0.05% (mass) of raw material after sodium percarbonate, calcium percarbonate catalytic oxidation reaction 3h, in belt sieve plate The first-stage precipitation separation is carried out in the reactor to remove the insoluble impurities of pharmaceutical fulvic acid; then the FX100370X1070 vertical cyclone, the LW255X765-NLWX020-N decanter screw separator is used for the second-stage separation, and the DHC300 disc centrifuge is used for the third-stage separation. High-grade liquid-solid separation to remove insoluble fine impurities in pharmaceutical fulvic acid; using 0.05% pharmaceutical grade chitosan (provided by the Chemistry and Molecular Engineering Research Office of East China University of Science and Technology), at a temperature of 50°C, for 1 hour, and a pressure of 0.12MPa Carry out gel hydr...

Embodiment 2

[0029]Take 100 parts of peat raw material, after screening and pulverizing, at a reaction temperature of 130°C and a reaction pressure of 0.13 MPa, use sodium percarbonate and calcium percarbonate with a concentration of 0.01% (mass) of the raw material to catalyze the oxidation reaction for 3 hours, and then carry out primary precipitation separation , to remove insoluble coarse impurities; then use FX100370X1070 vertical cyclone machine, LW255X765-NLWX020-N horizontal screw machine for liquid-solid separation, and DHC300 disc machine for re-separation to remove insoluble fine impurities in fulvic acid; use 0.05% pharmaceutical Grade chitosan (provided by the Chemistry and Molecular Engineering Research Office of East China University of Science and Technology), at a temperature of 50 ° C, for 1 hour, and a pressure of 0.12 MPa for deashing by gel hydrolysis phase transition method, after the effective rate reaches 96.0-98.5%, it is crude Fulvic acid pharmaceuticals, at a temp...

Embodiment 3

[0031] Get 100 parts of weathered lignite raw materials, after screening and pulverizing, at a reactor temperature of 130° C., under a reaction pressure of 0.13 MPa, adopt a concentration of 0.1% (mass) of raw materials for sodium percarbonate and calcium percarbonate to catalyze the oxidation reaction for 3 hours, and then use a sieve The first-stage precipitation separation is carried out in the reactor of the plate to remove the insoluble impurities of pharmaceutical fulvic acid; then the FX100370X1070 vertical cyclone, the LW255X765-NLWX020-N decanter screw separator is used for the second-stage separation, and the DHC300 disc centrifuge is used for further separation. Three-stage liquid-solid separation to remove insoluble fine impurities in pharmaceutical fulvic acid; using 0.05% pharmaceutical grade chitosan (provided by the Chemistry and Molecular Engineering Research Office of East China University of Science and Technology), at a temperature of 50°C, for 1 hour, and a ...

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Abstract

The method comprises the following steps: carrying out catalytic oxidation on peat, lignite and weathered coal humic acid, carrying out deep separation through a vertical cyclone machine, a horizontal spiral separator and a disc centrifuge, carrying out gel phase change deliming, carrying out continuous desalination through a water-alcohol membrane, a homogeneous phase and a heterogeneous electrodialysis membrane, and carrying out molecular weight determination to obtain the medical fulvic acid standard product. An inhibition experiment on transaminase of hepatoma carcinoma cells Hep1-3B is carried out. The fulvic acid can react with alpha-glucosidase, experiments for preventing pancreatic resistance and reducing blood sugar and survival rate in human brain glioma U87 cells prove that the drug effect reaches 35.28-80.00% or above, the fulvic acid has an adjusting effect on brain barriers, the additional value of fulvic acid pharmaceuticals is effectively improved, and the application prospect is wide.

Description

technical field [0001] The invention mainly relates to the manufacture of pharmaceutical fulvic acid standard products and the application to transaminase, blood sugar and tumor cell experiments. Background technique [0002] Fulvic acid is a kind of humic acid. The medical application of humic acid has been recorded since Tang Dynasty Liu Zongyuan’s "Answer to Cui Anshu". After the Medical Professional Committee of the Industry Association, in May 2018, the China Humic Acid Industry Association held the "First China Humic Acid Medicine and Health Industry Development Forum" in Liaocheng, Shandong, and it was broadened. Looking back from history, humic acid pharmaceutical products and markets, research on the safety, effectiveness and quality control of humic acid pharmaceuticals are necessary, but there are no standardized products and commodities of humic acid fulvic acid pharmaceuticals at home and abroad. [0003] In 1981, Yan Weide published a paper in Jiangxi Humic Ac...

Claims

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Application Information

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IPC IPC(8): C08H7/00C12Q1/02
CPCC08H6/00G01N33/5011G01N33/5008C12N2503/02G01N2500/10
Inventor 周霞萍崔军彦梁圣模沈天瑞崔刚彦梁琪翊赵日光潘君
Owner 上海臻衍生物科技有限公司
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