Method for extracting sea cucumber gonad polysaccharide by using microbial protease

A microbial protease and sea cucumber technology, applied in the field of resource utilization of sea cucumber waste, can solve the problem of less polysaccharides in the extraction of sea cucumber gonads

Pending Publication Date: 2021-12-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on the extraction of sea cucumber polysaccharides, but there are few studies on the extraction of sea cucumber gonad polysaccharides, especially about the use of microbial protease enzymatic hydrolysis to extract sea cucumber gonad polysaccharides with a high extraction rate.

Method used

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  • Method for extracting sea cucumber gonad polysaccharide by using microbial protease
  • Method for extracting sea cucumber gonad polysaccharide by using microbial protease
  • Method for extracting sea cucumber gonad polysaccharide by using microbial protease

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Embodiment Construction

[0015] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0016] 1. Material selection

[0017] Sea cucumber gonads were selected as raw materials for the preparation of polysaccharides.

[0018] 2. Pretreatment of sea cucumber gonads

[0019] Sea cucumber gonads are cleaned, freeze-dried, pulverized, and filtered through a 50-mesh sieve to obtain sea cucumber gonad powder.

[0020] 3. Degreasing and decolorization

[0021] Weigh 1g of sea cucumber gonad powder, add 30mL of chloroform-methanol (2:1), centrifuge at 6000rpm for 10min after 2h; add 30mL of acetone to the precipitate, centrifuge at 6000rpm for ...

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Abstract

The invention discloses a method for extracting sea cucumber gonad polysaccharide by using microbial protease. The method is characterized by comprising the following steps of: pretreating low-cost sea cucumber gonad serving as a raw material to obtain sea cucumber gonad powder, degreasing and decoloring the sea cucumber gonad powder, dissolving the sea cucumber gonad powder in a phosphate buffer solution according to a solid-liquid ratio of 1: 40, adjusting the pH value of the obtained solution to 7.5, adding the microbial protease, performing enzymolysis at 50 DEG C for 6 hours, sequentially using quaternary ammonium salt and ethanol to precipitate polysaccharide after enzyme deactivation, conducting centrifuging, dissolving a precipitate, performing dialyzing by using a 10-kDa dialysis bag, and carrying out freeze-drying to obtain the sea cucumber gonad polysaccharide. According to the method, the extraction rate of sea cucumber gonad polysaccharide is increased by screening enzymolysis conditions. Compared with an existing method, the method of the invention has the advantages that the extraction rate of the sea cucumber gonad polysaccharide is increased by 30.13%; and meanwhile, the problem that the environment is affected by byproducts in a sea cucumber processing process is solved, and the method has theoretical guiding significance for further increasing the comprehensive utilization value of sea cucumbers.

Description

technical field [0001] The invention relates to a method for extracting sea cucumber gonad polysaccharides by using microbial protease, and belongs to the field of resource utilization of sea cucumber waste. Background technique [0002] Sea cucumber (Sea cucumber) is a creature of Echinodermata (Holothuroidea), rich in biologically active substances, such as: polysaccharides, saponins, peptides, cerebrosides and sphingoids, etc., with anti-tumor, anti-oxidation, Anti-coagulation, lowering blood fat and enhancing the body's immune function and other effects. The sea cucumber industry is very developed in Asian countries, mainly concentrated in China, Japan and Southeast Asia. During sea cucumber processing, the internal organs of sea cucumbers are often discarded as waste, or simply processed as food, and their potential value is often ignored. In recent years, the sea cucumber processing industry has developed day by day, and people have gradually realized the potential e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 夏秀芳杜鑫潘男石硕万伟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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