Strain HSCY 2073 as well as separation and screening thereof and application of strain HSCY 2073 in improvement of flavor and quality of table vinegar
A bacterial strain and vinegar technology, applied in the preparation of vinegar, isolation of microorganisms, bacteria, etc., can solve the problems of poor high acid resistance and difficult growth and metabolism of lactic acid bacteria, achieve convenient operation, enhance flavor quality, and improve overall flavor quality Effect
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Embodiment 1
[0038] Isolation and Identification of Strain HSCY 2073
[0039] (1) Enrichment culture of bacterial strains
[0040]Collect Zhenjiang fragrant vinegar, Shanxi mature vinegar, Sichuan bran vinegar and other manufacturers and different batches of vinegar unstrained spirits that have just finished fermentation. Weigh 250g of each sample and add 250mL sterilized glucose aqueous solution, the glucose content is 2% (w / w), after the mouth was tied and sealed, it was placed in a 35°C incubator for static enrichment culture for 14 days.
[0041] (2) Isolation of strains
[0042] Take the above-mentioned enriched samples, take 25ml of each sample and add it to 225ml sterilized physiological saline, shake well, take 1ml each and add it to 9ml sterile physiological saline, mix evenly on a vortex mixer, and then take 1ml The diluted solution was added to 9ml of normal saline, and so on, diluted 7 times. The stock solution and all diluted samples were selected and spread onto the modif...
Embodiment 2
[0058] Intensive application of strain HSCY 2073 in brewing Zhenjiang fragrant vinegar
[0059] This example provides the enhanced application of the strain HSCY 2073 of the present invention in combination with traditional Zhenjiang balsamic vinegar seed fermented grains in the brewing of Zhenjiang balsamic vinegar.
[0060] (1) Preparation of seed solution
[0061] 1. Strain activation and expansion
[0062] Use an inoculation loop to inoculate the strain HSCY 2073 stored on a slope at 4°C into 10mL of sterilized modified liquid MRS medium, where the modified liquid MRS medium is adjusted to 5.0g / 100mL of total acid by acetic acid, and then kept at 30°C, sealed and allowed to stand Culture 10d. Then take 5mL of the above fermentation broth and transfer it into 100mL sterilized liquid MRS medium. Here, the improved liquid MRS medium is adjusted to 5.0g / 100mL of total acid by acetic acid, and then at 30°C, the number of viable bacteria is about 10 7 CFU / mL.
[0063] Strain...
Embodiment 3
[0093] Intensified Application of Strain HSCY 2073 in Shanxi Mature Vinegar Production
[0094] This example provides the enhanced application of the bacterial strain of the present invention combined with the traditional fermented grains of Shanxi mature vinegar in the brewing of Shanxi mature vinegar.
[0095] (1) Preparation of seed solution
[0096] Adopt the same method as above-mentioned embodiment 2 to carry out expansion cultivation.
[0097] (2) Intensified application in the brewing of traditional Shanxi mature vinegar
[0098] 1. Wine mash preparation
[0099] Take nine 500kg large vats, each take 100kg of crushed sorghum with only four to six petals, add 50kg of water to moisten the material for 15 hours. Steam sorghum with steam, add 300kg of water and stir, then cool to 30°C, then put 60kg of Daqu into it, and stir evenly. During the alcoholic fermentation process, stir irregularly according to the winemaking requirements, control the temperature at about 30°...
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