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Extraction method and application of tremella polysaccharide

An extraction method, tremella polysaccharide technology, which is applied in the field of traditional Chinese medicine, can solve the problems of further improvement in purity and high cost of enzymatic hydrolysis, and achieve good scavenging effect, high content, and improved extraction rate

Active Publication Date: 2021-11-26
金唐(福建)健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a high extraction rate of tremella polysaccharide, but the cost of enzymatic hydrolysis is high, and the purity needs to be further improved

Method used

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  • Extraction method and application of tremella polysaccharide
  • Extraction method and application of tremella polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting Tremella polysaccharide, characterized in that it comprises the steps of:

[0030] (1) Take the white mesophyll of fresh white fungus, weigh it, and then soak it with 3 times the amount of solvent for 30 minutes; add 35 times the volume of distilled water, then use a homogenizer to beat for 5 seconds and stop for 3 seconds, accumulatively beat for 1 minute; then at a speed of 8000r / min Centrifuge for 30min, take the supernatant 1;

[0031] The solvent is a mixed dispersion solution of sorbitol, polyethylene glycol, lecithin and water, and the mass ratio of sorbitol, polyethylene glycol 600, lecithin and water is 0.03:0.06:0.2:1; lecithin was purchased from Xi’an Jinxiang Pharmaceutical Excipients Co., Ltd.;

[0032] (2) Adding ammonium sulfate to the supernatant 1 until its saturation is 55%, adding sodium chloride to the supernatant 1 until its saturation is 40%, stirring for 10min, at a speed of 8000r / min Centrifuge for 10min, take the supern...

Embodiment 2

[0036] A method for extracting Tremella polysaccharide, characterized in that it comprises the steps of:

[0037] (1) Take the white mesophyll of fresh white fungus, weigh it, and then soak it with 1 times the amount of solvent for 20 minutes; add 25 times the volume of distilled water, then beat with a homogenizer for 5 seconds and stop for 3 seconds, accumulatively beat for 1 minute; then at a speed of 7000r / min Centrifuge for 20min, take supernatant 1; lecithin was purchased from Hebei Zhentian Food Additive Co., Ltd.;

[0038] The solvent is a mixed dispersion solution of sorbitol, polyethylene glycol, lecithin and water, and the mass ratio of sorbitol, polyethylene glycol 400, lecithin and water is 0.01:0.05:0.1:1.

[0039] (2) Adding ammonium sulfate to the supernatant 1 until its saturation is 60%, adding sodium chloride to the supernatant 1 until its saturation is 40%, stirring for 10min, at a speed of 9000r / min Centrifuge for 5 minutes, take the supernatant 2;

[00...

Embodiment 3

[0043] A method for extracting Tremella polysaccharide, characterized in that it comprises the steps of:

[0044] (1) Take the white mesophyll of fresh white fungus, weigh it, and then soak it with 3 times the amount of solvent for 40 minutes; add 45 times the volume of distilled water, then use a homogenizer to beat for 5 seconds and stop for 3 seconds, accumulatively beat for 0.5 minutes; then at a speed of 7000r / min Centrifuge for 40min, take the supernatant 1;

[0045] The solvent is a mixed dispersion solution of sorbitol, polyethylene glycol, lecithin and water, and the mass ratio of sorbitol, polyethylene glycol 600, lecithin and water is 0.05:0.08:0.3:1; lecithin was purchased from Xi’an Jinxiang Pharmaceutical Excipients Co., Ltd.;

[0046] (2) Adding ammonium sulfate to the supernatant 1 until its saturation is 55%, adding sodium chloride to the supernatant 1 until its saturation is 45%, stirring for 10min, at a speed of 7000r / min Centrifuge for 15min, take the sup...

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Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to an extraction method and application of tremella polysaccharide. The method comprises the following steps: (1) soaking tremella with a solvent; adding water, pulping, centrifuging, and taking supernate 1; (2) adding inorganic salt into the supernate 1, stirring, centrifuging, and taking supernate 2; (3) adding the supernate 2 into an ethanol-acetone aqueous solution, standing, and sieving with a 200-300-mesh sieve to obtain a polysaccharide precipitate; and (4) redissolving the polysaccharide precipitate, heating to remove the organic solvent, and freeze-drying in vacuum to obtain the polysaccharide. The extraction method is high in tremella polysaccharide extraction rate, high in purity, short in extraction time and beneficial to industrial large-scale production.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to an extraction method and application of tremella polysaccharide. Background technique [0002] Tremella, also known as white fungus, is a traditional nourishing health product in my country. Tremella has the effects of nourishing yin, moistening lungs, nourishing qi and blood. Tremella polysaccharides are derived from heteropolysaccharides isolated and purified from tremella fruit bodies and tremella cell submerged fermentation spores. A large number of studies have shown that most of the pharmacological activities of tremella are related to tremella polysaccharides. It has the functions of enhancing immunity, anti-aging, anti-tumor, reducing Blood sugar, blood fat lowering, anti-radiation and other physiological functions. At present, it is mainly used in cosmetics and food, but less in health products and medicines, and the medicinal value of white fungus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003A61P39/06
Inventor 林枝东
Owner 金唐(福建)健康科技有限公司
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