Preparation method of duck liver positive serum

Abstract

The invention discloses a duck liver positive serum preparation method, and belongs to the technical field of virus detection. The duck liver positive serum preparation method comprises the steps that Step 1, materials are prepared, and a virus strain, an inactivated vaccine and a chick embryo are optimized; Step 2, immune serum is produced in a trail manner, the inactivated vaccine is injected into the chick embryo, so that the chick embryo generates immune reaction, and finally, the chick embryo is promoted to generate the immune serum; Step 3, a safety test and an effect test are performed on the chick embryo by using the immune serum; Step 4, the immune serum is prepared on a large scale; Step 5, product quality inspection and safety inspection are performed on the prepared immune serum; and Step 6, the immune serum is efficiently stored. According to the invention, the neutralizing reaction with the type 3 duck hepatitis virus is poor, the titer of the neutralizing antibody is 1:2896, the titer of the neutralizing antibody is stable after the repeated test, the antibody can be used for inspection, and during neutralization of the virus liquid with different contents by using the serum with different neutralizing antibody titers, and the virus liquid with the concentration of 104.0 ELD50 / 0.1 ml can be completely neutralized when the titer of the neutralizing antibody is 1:2048 according to the result.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a method for preparing duck liver positive serum. Background technique [0002] Duck Virus Hepatitis (DVH) is an acute, highly contagious and fatal infectious disease of ducklings caused by Duck Hepatitisvirus (DHV), characterized by hemorrhagic inflammation of the liver , the disease can occur throughout the year, and only occurs in ducklings during natural outbreaks, causing huge economic losses to the breeding industry. Immunization with live vaccines is still one of the most important measures to prevent and control the disease. The identification test is the virus species and One of the key points of live vaccine quality monitoring. [0003] Most of the currently used vaccines have the following disadvantages. First, the antibody level of duck immune serum will gradually decrease within 24 months before the slaughter or the peak egg production period, which in turn ...

AI Technical Summary

Problems solved by technology

[0003] Most of the currently used vaccines have the following disadvantages. First, the antibody level of duck immune serum will gradually decrease within 24 months before the slaughter or the peak egg production period, which in turn causes the need for replanting the vaccine. Secondly, in the actual inspection process, the actual antibody concentration of duck immune serum is not high, and the neutralization efficiency is further reduced

Images