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Copuloplasmin detection kit and preparation method thereof

A detection kit, ceruloplasmin technology, applied in the field of medical detection, can solve the problems of multi-antibody serum differences, affecting the performance of reagents, etc., and achieve the effects of excellent stability, improvement of batch differences, and improved stability.

Pending Publication Date: 2021-11-23
北京安图生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polyantiserum, the key raw material used in ordinary immunoturbidimetry, often has differences in different batches of polyantiserum due to some uncontrollable factors, which affects the performance of the reagent.
And ceruloplasmin polyantiserum can not maintain long-term stability in the buffer (18 months)

Method used

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  • Copuloplasmin detection kit and preparation method thereof
  • Copuloplasmin detection kit and preparation method thereof
  • Copuloplasmin detection kit and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0050] The preparation of embodiment 1 kit of the present invention

[0051] The kit of the present invention is based on the common immunoturbidimetric method for measuring CER, and consists of reagent R1 and reagent R2, wherein the buffer in reagent R1 is 10 mM PB buffer with pH 7.4. Reagent R2 is sucrose. However, the components of reagent R2 used 3 different batches of ceruloplasmin polyantiserum (Origene, lot#F00702-01; C14503R-01; C14503-01), marked as batch 1, batch 2, batch 3.

[0052] Table 1 Components of Reagent R1

[0053]

[0054]

[0055] The preparation steps of reagent R1 are (preparation of 1L): according to the component concentrations of Examples 1-3 in Table 1 and the component content of the above reagent R1, prepare PB buffer solution with purified water, adjust the pH, and use it as R1 buffer solution ; Then add the corresponding amount of sodium chloride, Tween 20, polyethylene glycol 6000 and ProClin300, mix well and adjust the pH with sodium hy...

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Abstract

The invention relates to the technical field of medical detection, in particular to a cupreuloplasmin detection kit. The kit consists of a reagent R1 and a reagent R2, the reagent R1 is prepared from 5-30 m of MPB buffer solution with the pH value of 7.0-8.0, 9-30 g / L of inorganic salt, 40-80 g / L of coagulant, 0.5-2 g / L of surfactant and 0.1-1 g / L of preservative; the R2 reagent consists of a 5-100 mM buffer solution with the pH value of 7.0-8.0, 9-30 g / L of inorganic salt, 100-500 g / L of copper cyanin polyclonal antibody serum, 0-5 g / L of a protective agent, 60-80 g / L of a stabilizer and 0.1-1 g / L of a preservative; the stabilizer in the R2 is one or more of cane sugar, trehalose, glycerol and glucose. Experiments show that the kit is good in stability, small in batch difference, high in sensitivity and beneficial to wide clinical popularization and application.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a ceruloplasmin detection kit and a preparation method thereof. Background technique [0002] Ceruloplasmin (ceruloplasmin, CER), also known as hemocyanin and copper oxidase, is a copper-containing α2-globulin with oxidase, and its relative molecular mass is 151×10 3 , each molecule contains 6-7 copper atoms, the copper content is 0.34%, about 95% of the copper in the serum is combined with α2-globulin to form ceruloplasmin, which regulates iron absorption and transport, and has ferrous oxidase and amine chlorase activity. Its main physiological functions include transporting copper and iron oxide, promoting cell growth and angiogenesis, and copper is one of the essential trace elements in the human body, has important biological functions, and has mutual regulation with thyroxine. It can promote the decomposition of histamine, serotonin, etc., and can form the body's...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/539
CPCG01N33/573G01N33/539G01N2333/90287Y02A50/30
Inventor 李媛媛
Owner 北京安图生物工程有限公司
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