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Preparation method and application of micromolecular dendrobium officinale polysaccharide

A technology of Dendrobium officinale polysaccharide and Dendrobium officinale can be applied to skin care preparations, medical preparations containing active ingredients, skin diseases, etc. Small, good anti-inflammatory effect

Pending Publication Date: 2021-11-19
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dendrobium officinale polysaccharides prepared by techniques in the prior art cannot meet the requirements of small molecular weight, extraction rate and purity

Method used

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  • Preparation method and application of micromolecular dendrobium officinale polysaccharide
  • Preparation method and application of micromolecular dendrobium officinale polysaccharide

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Embodiment 1

[0049] The invention discloses a preparation method of small molecular dendrobium officinale polysaccharides. The dry product of dendrobium officinale is used as a starting material and extracted by a deep eutectic solvent combined with a subcritical method. Adding a deep eutectic solvent to the medicinal dregs of Dendrobium officinale, performing subcritical extraction to obtain an initial filtrate, and then undergoing enzymatic hydrolysis, elution, concentration, and precipitation to obtain a finished small molecule polysaccharide of Dendrobium officinale.

[0050] Wherein, the hydrogen bond acceptor of the deep eutectic solvent is betaine, the hydrogen bond donor is glycerol, propylene glycol or butanediol, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1˜1:5.

[0051] The preparation method of the small molecule dendrobium officinale polysaccharide of the present embodiment specifically comprises the following steps:

[0052] Step 1, pass...

Embodiment 2

[0063] A preparation method of small molecule dendrobium officinale polysaccharide, comprising the steps of:

[0064] Step 1: Grinding the dry product of Dendrobium officinale, putting the crushed Dendrobium officinale fine powder into an extraction tank, using steam to steam the Dendrobium officinale fine powder, and then continuously injecting high-temperature steam to soak it, and after cooling down to normal temperature, add anhydrous Stand still after stirring with ethanol, filter to obtain medicinal residues;

[0065] Step 2: Carry out multiple subcritical extractions on the dregs of step 1, add a deep eutectic solvent for each extraction, filter and collect the extract after each extraction, and finally combine all the extracts, and then pass the extract through a tubular centrifuge Collect by centrifugation to obtain the initial filtrate;

[0066] Step 3. Perform multiple high-pressure homogenization treatments on the initial filtrate of step 2. After the last high-pr...

Embodiment 3

[0074] A preparation method of small molecule dendrobium officinale polysaccharide, comprising the steps of:

[0075] Step 1. Grind the dry product of Dendrobium officinale, pass through a 40-100-mesh sieve to obtain fine powder of Dendrobium officinale, put the fine powder of Dendrobium officinale into an extraction tank, steam and soak for 1min-10min, and then continuously pass through 2m / s~30m / s high-temperature steam treatment for 5min~40min, after cooling down to normal temperature, add absolute ethanol with a mass ratio of 1 to 15 times that of Dendrobium candidum powder, stir, let stand for 1h~10h, filter to obtain medicinal residues;

[0076] Step 2: Carry out 2 to 5 subcritical extractions of the dregs of step 1, add a deep eutectic solvent for each extraction, extract according to the subcritical extraction parameters, and filter and collect the extract after each extraction is completed, and finally All extracts are combined, and the extracts are centrifuged throug...

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Abstract

The invention relates to a preparation method of micromolecular dendrobium officinale polysaccharide. A dendrobium officinale dried product is used as a starting raw material, and the micromolecularden drobium dendrobium officinale polysaccharide is obtained through a deep eutectic solvent and subcritical extraction. According to the preparation method of the micromolecular dendrobium officinale polysaccharide, the dendrobium officinale polysaccharide with the molecular weight smaller than or equal to 50 kD can be prepared, and the dendrobium officinale polysaccharide with the molecular weight smaller than or equal to 50 kD further has the advantages of being high in purity and high in extraction rate. The micromolecular dendrobium officinale polysaccharide with the molecular weight smaller than or equal to 50 kDa is small in molecular weight, high in purity, beneficial to skin absorption, capable of remarkably improving the skin immune barrier effect and good in anti-inflammatory effect.

Description

technical field [0001] The invention relates to the technical field of extraction of functional components of natural products, in particular to a preparation method of small molecule dendrobium officinale polysaccharide and its application. Background technique [0002] Dendrobium candidum is a perennial epiphytic herb of the same origin as medicine and food. It is also one of the species of Dendrobium included in the 2020 edition of "Chinese Pharmacopoeia". It is known as "the first of the nine Chinese immortal grasses". There are about 1,000 species, and there are 74 species in my country. The main producing areas in my country are Zhejiang, Yunnan, Anhui and other places, among which Yunnan has the largest planting area. [0003] Traditional Chinese medicine and clinical medicine studies believe that Dendrobium officinale has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, increasing immunity, and anti-tumor. It has been...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04C12P19/14A61K31/715A61K36/8984A61P17/00A61K8/73A61Q19/00
CPCC08B37/0003C12P19/04C12P19/14A61K31/715A61K36/8984A61P17/00A61P29/00A61K8/73A61Q19/00A61K2800/805
Inventor 刘光荣李莉楠杨凯业杜志云林丽马诗经
Owner INFINITUS (CHINA) CO LTD
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