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A method for extracting rubusoside and sweet tea polyphenols without bitterness and astringency from sweet tea leaves

A technology of bitterness and rubusoside, applied in the field of plant extraction and separation, to achieve comprehensive utilization and significant biological activity

Active Publication Date: 2022-06-21
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0045] To sum up, there are more or less deficiencies in the taste and mouthfeel improvement technologies of extracting rubusoside and sweet tea polyphenols from sweet tea leaves, and debittering of rubusoside, which are not ideal, and there is still a lot of room for improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] (1) Synergistic pretreatment of pulsed electric field and ultrasound. Take 200kg of dry sweet tea leaves, add 2000L tap water to soak the leaves, stir evenly, soak for 2 hours until the leaves stretch. At a solution temperature of 47°C, the samples were first treated with a pulsed electric field of 1.5kv / cm for 25 times, and then ultrasonically treated with a frequency of 20kHz and a power of 500W for 45min.

[0104] (2) Cyclic extraction. Put the pretreatment material into 3m 3 Multifunctional extraction tank, keep the temperature at 47 °C, discharge material at the bottom, turn on the circulation pump, and pump the solution flowing out from the bottom to the top of the tank, so that it passes through the material layer at a flow rate of 1500L / h, and is recycled for 3 times. The first 45min; the second time adding 1500L tap water, extraction 90min; the third time adding 1800L tap water, extracting 60min. The third extraction solution was used as a set of extraction,...

Embodiment 2

[0119] (1) Synergistic pretreatment of pulsed electric field and ultrasound. Take 500kg of dry sweet tea leaves, add 4500L tap water to soak the leaves, stir evenly, soak for 2.5h until the leaves stretch. At a solution temperature of 45°C, the cells were first treated with a pulsed electric field of 1.9kv / cm for 26 times, and then ultrasonically treated with a frequency of 40kHz and a power of 400W for 50min.

[0120] (2) Cyclic extraction. Put the pre-processing material into 6m 3 Multifunctional extraction tank, keep the temperature at 45°C, discharge material at the bottom, turn on the circulation pump, and pump the solution flowing out from the bottom to the top of the tank, so that it passes through the material layer at a flow rate of 4000L / h, and is recycled for 3 times. The first time 45min; the second time adding 4000L tap water, extraction 90min; the third time adding 4000L tap water, extracting 60min. The third extraction solution was used as a set of extraction...

Embodiment 3

[0135] Other conditions and operations are the same as in Example 1, the difference is that in step (1), the solution temperature is 40 ° C, and 9.15kg of rubusoside product is finally obtained, wherein the content of rubusoside is 93.27%, the sweet tea polyphenol is 1.31%; the sweet tea polyphenol product is 13.96% kg, of which the content of sweet tea polyphenols is 86.84%, and the flavonoids are 7.82%. After calculation, the total yield of sweet tea glycosides is 81.90%, and the total yield of sweet tea polyphenols is 73.75%.

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Abstract

The invention relates to a method for extracting rubusoside and polyphenols without bitterness and astringency from sweet tea leaves, which is characterized in that it comprises the following steps: (1) synergistic pretreatment of sweet tea leaves with pulsed electric field and ultrasound; (2) cyclic extraction; ( 3) filtering and clarifying to obtain clarified liquid; (4) passing clarified liquid through ultrafiltration membrane, ultrafiltration membrane permeate through nanofiltration membrane, collecting nanofiltration membrane retentate; (5) anion exchange resin separation; (6) anion exchange The resin column feed effluent and the washing liquid are combined and processed to obtain rubusoside; (7) the desorption solution obtained in step (5) is adjusted to weak acidity or neutrality with dilute acid, and the macroporous adsorption resin column of step (6) flows out The liquid and the initial water washing liquid are combined, and the sweet tea polyphenols are obtained after post-processing. The method of the invention realizes the enrichment of effective components and at the same time combines and processes the feed liquid containing other components, while realizing good separation, improves the product yield, realizes the comprehensive utilization of raw materials, and has industrial applicability.

Description

technical field [0001] The invention relates to the technical field of plant extraction and separation, in particular to a method for extracting sweet tea glycosides and sweet tea polyphenols from sweet tea leaves. Background technique [0002] The sweet tea described in the present application is the leaves of the genus Rubus rubus, scientific name: Rubus Suatrssimus S. Lee, English name: Sweet tea, also known as Guangxi sweet tea, Jinxiu wild sweet tea, Shunhuangshan sweet tea, etc. [0003] 1. Chemical composition and biological activity of sweet tea [0004] 1. Chemical composition [0005] Sweet tea contains a variety of chemical components: mainly contains 4-6% sweet tea glycosides, 8-18% sweet tea polyphenols, and 2.9-4.2% flavonoids. It also contains 18 kinds of amino acids, with a total amino acid content of 331.54 mg per 100 grams of dry product; calcium, zinc, germanium, selenium, and potassium, magnesium, phosphorus, iron, sodium, copper, chromium, strontium, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H15/256C07G99/00
CPCC07H1/08C07H15/256C07G99/00
Inventor 刘庚贵黄华学曾润清何安乐熊瑶黄俊陈明明
Owner HUNAN HUACHENG BIOTECH
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