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Low-cost method suitable for rapidly detecting aflatoxin in large-batch edible oil samples

A kind of aflatoxin and edible oil technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of excessive consumption of organic solvents and cumbersome steps, and achieve the effects of environmental friendliness, good recovery rate, and stable structure

Active Publication Date: 2021-10-22
INST OF AGRI QUALITY STANDARDS & TESTING TECH RES HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a low-cost method suitable for rapid detection of aflatoxins in large quantities of edible oil samples, and realize the detection of aflatoxins in edible oils by one-step solid-phase extraction technology. The rapid enrichment and purification of toxins avoids the shortcomings of traditional liquid-liquid extraction and gel permeation chromatography, which consume a lot of organic solvents and cumbersome steps. Combined with the universal liquid chromatography-fluorescence detector to separate and detect aflatoxins, it greatly improves Reduce the cost of sample analysis and realize the rapid detection of large quantities of samples at low cost

Method used

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  • Low-cost method suitable for rapidly detecting aflatoxin in large-batch edible oil samples
  • Low-cost method suitable for rapidly detecting aflatoxin in large-batch edible oil samples
  • Low-cost method suitable for rapidly detecting aflatoxin in large-batch edible oil samples

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Embodiment 1

[0036] A low-cost method suitable for the rapid detection of aflatoxins in large quantities of edible oil samples, such as figure 1 As shown, the steps are as follows:

[0037] 1) Weigh 0.5 g of the edible oil sample to be tested and place it in a 5 mL centrifuge tube, dilute the above edible oil sample to be tested with 3 mL of n-hexane, vortex for 1 min, and set aside;

[0038] 2) Take the pine pollen SPE cartridge (500mg, 6mL), activate it with 3mL of n-hexane before use, transfer the sample solution obtained in step 1) into the SPE cartridge, discard the effluent, and realize the extraction of aflatoxin in the sample solution enrichment;

[0039] 3) Continue to add 5mL of n-hexane to rinse the SPE column, then use 5mL of acetone to desorb, and the desorption solution N 2 Blow dry, redissolve with 1.0mL acetonitrile-water (50 / 50, v / v), the obtained solution is the test solution for rapid determination of aflatoxin in edible oil, and perform subsequent HPLC-FLD analysis, t...

Embodiment 2

[0042] In order to verify the feasibility of the low-cost method provided by the present invention that is suitable for the rapid detection of aflatoxin in large quantities of edible oil samples in practical applications, the present embodiment is linear, detection limit (LODs), limit of quantitation ( LOQs) and other parameters were investigated. Two kinds of aflatoxins were prepared into a series of standard mixed solutions of 0.1-20 μg / kg, and analyzed using the HPLC-FLD conditions in Example 1. The concentration of each component was taken as the abscissa, and the corresponding peak area was taken as the ordinate, Establish a calibration curve for aflatoxins. As shown in Table 1, the two aflatoxins have a good linear relationship in the range of 0.1-20 μg / kg, and the square of the regression coefficient (R 2 ) is greater than 0.9987; the detection limit and quantification limit of the two aflatoxins calculated by 3 times and 10 times the signal-to-noise ratio are in the r...

Embodiment 3

[0046] In order to evaluate the accuracy and precision of the low-cost method suitable for rapid detection of aflatoxins in large quantities of edible oil samples provided by the present invention, the recovery rate of the method was investigated in this embodiment. Specific steps are as follows:

[0047] 1) Weigh 0.5g of edible oil sample and place it in a 5mL centrifuge tube, add three concentrations of aflatoxin in low, medium and high concentrations as the edible oil sample to be tested, vortex and mix well, let stand for 30min, and dilute the above-mentioned aflatoxin with 3mL of n-hexane The edible oil sample to be tested, and vortexed for 1min, set aside;

[0048] 2) Take the pine pollen SPE cartridge (500 mg, 6 mL), activate it with 3 mL of n-hexane, transfer the sample solution obtained in step 1) into the SPE cartridge, discard the effluent, and extract and enrich the aflatoxin in the sample solution;

[0049] 3) Add 5 mL of n-hexane to rinse the SPE column, then de...

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Abstract

The invention discloses a low-cost method suitable for rapidly detecting aflatoxin in a large batch of edible oil samples, which comprises the following steps: 1) diluting an edible oil sample to be detected with n-hexane, loading the sample through a pine pollen solid-phase extraction small column, and extracting and enriching the aflatoxin in the sample; 2) after sample loading is finished, cleaning the pine pollen solid-phase extraction small column to remove interferents, desorbing and redissolving to obtain a solution which is a to-be-detected solution of aflatoxin in edible oil; and (3) rapidly detecting the content of aflatoxin in the sample by adopting an external standard method and a high performance liquid chromatography-fluorescence detector. In the pretreatment process, a large amount of matrix in the edible oil can be removed, the purification effect is good, and the recovery rate of aflatoxin is very good; the aflatoxin is separated and detected by combining a universal and low-cost liquid chromatography-fluorescence detector, low-cost rapid detection of a large batch of edible oil samples can be realized, the detection sensitivity and accuracy are high, and reproducibility is good.

Description

technical field [0001] The invention belongs to the technical field of rapid analysis and detection, and in particular relates to a low-cost method suitable for rapid detection of aflatoxins in large batches of edible oil samples. Background technique [0002] Aflatoxins are one of the most common mycotoxins that contaminate agricultural products and food. A class of toxic secondary metabolites produced by toxigenic fungi infecting their hosts. It has been detected in more than 110 kinds of agricultural products and foods such as peanuts, corn, rice, nuts, cottonseed and dairy products, among which the pollution of grain and oil products such as peanuts and corn is the most serious. Aflatoxin has a stable molecular structure and high temperature resistance (melting point is 237°C to 299°C), and is difficult to be destroyed under normal cooking and processing temperatures. [0003] At present, more than 20 kinds of aflatoxins and their derivatives have been isolated, the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 彭西甜郑丹胡西洲张惠贤夏虹周有祥彭立军
Owner INST OF AGRI QUALITY STANDARDS & TESTING TECH RES HUBEI ACADEMY OF AGRI SCI
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