Glutathione reductase detection kit as well as preparation method and application thereof

A detection kit, glutathione technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of low sensitivity, poor on-board stability of the kit, weak antibacterial ability, etc. PH drop speed, the effect of ensuring a stable PH environment

Pending Publication Date: 2021-10-22
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has announced its actual preparation and application, but the kit has poor airborne stability, low sensitivity, and weak antibacterial ability

Method used

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  • Glutathione reductase detection kit as well as preparation method and application thereof
  • Glutathione reductase detection kit as well as preparation method and application thereof
  • Glutathione reductase detection kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]A glutathione reductase detection kit includes reagents R1 and R2, wherein reagent R1 includes: potassium phosphate buffer 100mmol / L, EDTA 1mmol / L, GSSG 1mmol / L, ascorbate oxidase 3KU / L, bilirubin Oxidase 1KU / L, potassium ferricyanide 0.1g / L, Triton 100 0.1%, Tween 20 0.5%, water-soluble amphotericin B, vancomycin hydrochloride; the reagent R2 includes sodium bicarbonate buffer 50mmol / L , NADPH 2g / L, water-soluble amphotericin B, Triton 1000.1%, Tween 20 0.5%, bovine serum albumin 1g / L, sorbitol 2g / L, riboflavin 0.5g / L.

Embodiment 2

[0041] A glutathione reductase detection kit includes reagents R1 and R2, wherein reagent R1 includes: potassium phosphate buffer 50mmol / L, EDTA 1.5mmol / L, GSSG 1.5mmol / L, ascorbate oxidase 3KU / L, Bilirubin oxidase 1KU / L, potassium ferricyanide 0.1g / L, Brij35 100 0.1%, Tween 20 0.5%, water-soluble amphotericin B, vancomycin hydrochloride; the reagent R2 includes sodium bicarbonate buffer 80mmol / L, NADPH 2g / L, water-soluble amphotericin B, Triton 1000.1%, Tween 20 0.5%, bovine serum albumin 1.5g / L, sorbitol 2.5g / L, riboflavin 0.5g / L.

Embodiment 3

[0043] A glutathione reductase detection kit includes reagents R1 and R2, wherein reagent R1 includes: potassium phosphate buffer 50mmol / L, EDTA 1.5mmol / L, GSSG 1.5mmol / L, ascorbate oxidase 3KU / L, Bilirubin oxidase 1KU / L, potassium ferricyanide 0.1g / L, Triton 405 0.1%, Tween 20 0.5%, water-soluble amphotericin B, vancomycin hydrochloride; the reagent R2 includes sodium bicarbonate buffer 80mmol / L, NADPH 2g / L, water-soluble amphotericin B, Triton 4050.1%, Tween 20 0.5%, bovine serum albumin 1g / L, sorbitol 2g / L, riboflavin 0.5g / L.

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PUM

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Abstract

The invention provides a glutathione reductase detection kit and a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a potassium phosphate buffer solution, EDTA, GSSG, ascorbic acid oxidase, bilirubin oxidase, potassium ferricyanide, a surfactant, a first bacteriostatic agent and a second bacteriostatic agent; and the reagent R2 comprises a sodium bicarbonate buffer solution, NADPH, the first bacteriostatic agent, a surfactant and a stabilizer. Compared with the prior art, the glutathione reductase detection kit provided by the invention has the advantages of good airborne stability, high sensitivity and strong antibacterial ability, and can meet requirements of clinical application of glutathione reductase detection.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a glutathione reductase detection kit and its preparation method and application. Background technique [0002] Glutathione reductase (GR) is one of the important enzymes in the redox system of the human body. Reduced coenzyme II (NADPH) acts as a hydrogen donor to reduce oxidized glutathione (GSSG) to redox under the catalysis of GR. Prototype glutathione (GSH), GSH can prevent the oxidative decomposition of hemoglobin and maintain the integrity of cells. GR can reflect liver injury in the early stage, and the period of acute hepatitis is earlier than transaminase response. At the same time, GR can also reflect the type of jaundice, and the GR activity of non-hepatic jaundice is at a normal level. [0003] At present, the methods for detecting glutathione reductase include ELISA method and ultraviolet enzymatic method. Among them, ELISA method is cumbersome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26
CPCC12Q1/26
Inventor 邹炳德邹继华梅霞汪屹贾江花
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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