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Preparation method of oriented nanofiber nerve conduit

A nerve conduit and nanofiber technology, used in pharmaceutical formulations, medical science, prostheses, etc., can solve the problems of easily causing inflammation in surrounding tissues, slow degradation rate, and acidic degradation products, achieving excellent biocompatibility, promoting The effect of repair and preparation method is simple

Pending Publication Date: 2021-10-19
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polylactic acid (PLA) is a synthetic degradable material, which is non-toxic, strong plasticity, good mechanical properties and biocompatibility, but the degradation rate is slow, and the degradation products are acidic, which is easy to cause damage to surrounding tissues. Inflammation, generally need to compound other materials

Method used

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  • Preparation method of oriented nanofiber nerve conduit
  • Preparation method of oriented nanofiber nerve conduit
  • Preparation method of oriented nanofiber nerve conduit

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for preparing an oriented nanofiber nerve guide, comprising the following steps:

[0033] 1) The directional fiber nerve conduit was prepared by electrospinning technology. Take 0.3g of gelatin, mix it with polylactic acid at a mass ratio of 1:1, dissolve it in 10ml of hexafluoroisopropanol to make a spinning solution, then add 40ng of nerve growth factor NGF and 10mg of astragalus polysaccharide, stir magnetically for 0.5-2h and then sonicate Degas for 10 minutes; add the above spinning solution into a 10ml syringe, connect a 21G stainless steel nozzle, set the distance between the nozzle and the receiving plate to 15cm, use a voltage of 9-12kV, a negative voltage of 3KV, a flow rate of 1.0ml / h, and a receiving speed of 2400r / min as the condition for electrospinning. The prepared samples were vacuum dried for 24 hours to remove residual organic solvents.

[0034] figure 1 This is the SEM picture of the prepared nerve guide, in which the fibers are oriented...

Embodiment 2

[0070] A method for preparing an oriented nanofiber nerve guide, comprising the following steps:

[0071] 1) The directional fiber nerve conduit was prepared by electrospinning technology.

[0072] Take 0.3g of polylactic acid, mix it with gelatin at a mass ratio of 2:1, dissolve it in 10ml of hexafluoroisopropanol to make a spinning solution, then add 30ng of nerve growth factor NGF and 15mg of astragalus polysaccharide, stir magnetically for 0.5-2h and then sonicate Degas for 10 minutes; add the above spinning solution into a 10ml syringe, connect a 21G stainless steel nozzle, set the distance between the nozzle and the receiving plate to 15cm, use a voltage of 9-12kV, a negative voltage of 3KV, a flow rate of 1.0ml / h, and a receiving speed of 2400r / min as the condition for electrospinning. The prepared samples were vacuum dried for 24 hours to remove residual organic solvents.

[0073] 2) Synthesis and cross-linking of nerve conduits

[0074] The length of the nerve gui...

Embodiment 3

[0076] A method for preparing an oriented nanofiber nerve guide, comprising the following steps:

[0077] 1) The directional fiber nerve conduit was prepared by electrospinning technology.

[0078] Mix 0.3g of polylactic acid and gelatin at a mass ratio of 4:1, dissolve in 10ml of hexafluoroisopropanol to make a spinning solution, then add 60ng of nerve growth factor NGF and 8mg of astragalus polysaccharide, stir magnetically for 0.5-2h, and then ultrasonically remove Gas for 10 minutes; add the above spinning solution into a 10ml syringe, connect a 21G stainless steel nozzle, set the distance between the nozzle and the receiving plate to 15cm, use a voltage of 9-12kV, a negative voltage of 3KV, a flow rate of 1.0ml / h, and a receiving speed of 2400r / h min as the condition for electrospinning. The prepared samples were vacuum dried for 24 hours to remove residual organic solvents.

[0079] 2) Synthesis and cross-linking of nerve conduits

[0080] The length of the directiona...

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Abstract

The invention discloses a preparation method of an oriented nanofiber nerve conduit, which comprises the following steps: (1) blending polylactic acid and gelatin, and dissolving in a hexafluoroisopropanol solution to prepare an electrostatic spinning solution; (2) adding nerve growth factors and astragalus polysaccharide into the electrostatic spinning solution; (3) preparing an oriented nanofiber nerve conduit by using an electrostatic spinning method; and (4) adding the nerve conduit into 0.1-2% of genipin solution for cross-linking, washing, and freeze-drying to obtain the nerve conduit. The nerve conduit prepared by the method has excellent biocompatibility, mechanical property and degradation property, and the nanofibers have directional distribution signals and can guide nerve cell axon extension. According to the preparation method, the synergistic effect of the medicine on promoting nerve regeneration is achieved, the nerve repairing rate is greatly increased, the astragalus polysaccharide can provide a good microenvironment for growth, proliferation and differentiation of nerve cells, and the occurrence of inflammatory reaction is reduced.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method of an oriented nanofiber nerve guide. Background technique [0002] Repair of peripheral nerve injury is a challenging medical problem. Autologous nerve transplantation is the "gold standard" in peripheral nerve repair due to its non-immunogenic effects, but there is also limited supply of donor tissue, neuroma formation risks, size mismatch, and issues of axonal distribution between the donor nerve and the site of injury. Nerve guides are artificial tissue-engineered tubes used to bridge stumps of nerves as an alternative to autologous nerve transplantation. However, the lack of effective biocompatibility and bioactivity is the main drawback of most artificial nerve guides. [0003] Commonly used preparation methods for nerve guides include casting molding, solution impregnation, melt extrusion, film laying, and electrospinning. Among them, the cathete...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/50A61L27/54A61L27/58
CPCA61L27/18A61L27/222A61L27/54A61L27/227A61L27/58A61L27/50A61L2400/12A61L2430/32A61L2300/414A61L2300/232C08L67/04
Inventor 许沛虎马毓聪徐海星李政林跃辛洋
Owner WUHAN UNIV OF TECH
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