Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of terpenoid THXM 88-3-3 in peach blossom tree fruit as tumor multidrug resistance reversal agent

A multi-drug resistance, multi-drug resistance technology, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of limiting the effectiveness of cancer chemotherapy and reducing the effectiveness of intracellular anti-cancer drugs. concentration, etc.

Pending Publication Date: 2021-10-19
NANKAI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the reason for multidrug resistance mainly comes from the high expression of ABC transporter in tumor cells. The transporter excretes the drug out of the cell, thereby reducing the effective concentration of intracellular anticancer drugs, and making cancer cells cross-responsive to multiple drugs. Drug resistance, a major barrier limiting the effectiveness of cancer chemotherapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of terpenoid THXM 88-3-3 in peach blossom tree fruit as tumor multidrug resistance reversal agent
  • Application of terpenoid THXM 88-3-3 in peach blossom tree fruit as tumor multidrug resistance reversal agent
  • Application of terpenoid THXM 88-3-3 in peach blossom tree fruit as tumor multidrug resistance reversal agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: THXM 88-3-3 affects the drug sensitivity of human cervical cancer drug-resistant cell line Hela / ddp to doxorubicin and mitoxantrone.

[0018] Hela cells in the logarithmic growth phase and its drug-resistant cell line Hela / ddp cells were inoculated in a 96-well plate at a seeding density of about 2,500 cells per well, and 36 wells around the periphery of the 96-well plate were not plated with cells. Only add PBS. Add 100 μL of DMEM medium containing 10% fetal bovine serum to each well, and place the 96-well plate at 37°C, 5% CO 2 and 100% humidity for 24 hours.

[0019] Three replicate wells were set up for each sample, and the experiment was repeated three times.

[0020] In this study, P-gP was mainly used as the research object, Verapamil was used as the standard reversal agent, and the chemotherapy drugs were doxorubicin and mitoxantrone.

[0021] Chemotherapy drugs are added. The chemotherapeutic drugs doxorubicin and mitoxantrone were prepared into ...

Embodiment 2

[0037] Example 2: THXM 88-3-3 affects the drug resistance of human chronic myeloid leukemia drug-resistant cell line K562 / A02 to doxorubicin.

[0038] K562 cells in the logarithmic growth phase and its drug-resistant cell line K562 / A02 cells were seeded in 96-well plates at a seeding density of about 1×10 per well. 4 cells, 36 wells around the periphery of the 96-well plate were not layered with cells, and only PBS was added. Add 100 μL of RPMI 1640 medium containing 10% fetal bovine serum to each well.

[0039] Three replicate wells were set up for each sample, and the experiment was repeated three times.

[0040] In this study, P-gP was mainly used as the research object, Verapamil was used as the standard reversal agent, and the chemotherapy drug was doxorubicin.

[0041] Chemotherapy drugs are added. The chemotherapeutic drug doxorubicin was prepared as a stock solution with different concentration gradients.

[0042] Add chemotherapy drugs into the 96-well plate accor...

Embodiment 3

[0054] Implementation Example 3: Effect on P-glycoprotein protein efflux function

[0055] The effect of BWB74-5-5 on the efflux function of MDR1 protein can change the fluorescence intensity of the corresponding fluorescent dye in the cell.

[0056] Hela and hela / dpp cells were cultured at a density of 1X10 4 Inoculate each well into a 96-well plate with a volume of 200 μL per well, set up 96 triplicates, and add sterilized distilled water to a circle around the 96-well plate. The 96-well plate was placed in a 37°C, 5% CO2 constant temperature incubator for 96 hours.

[0057] The reverse agent standard Verapamil was prepared to be 2mM, the dye Rhodamine123 was prepared to be 50ug / ml, and the test sample BWB74-5-5 was prepared to be 2.5mM.

[0058] After the standard substance and the sample BWB74-5-5 to be tested were added, they were placed in a 37°C, 5% CO2 constant temperature incubator and incubated for 15 minutes.

[0059] After 15 min, fluorescent dye was added and i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, relates to a novel application of THXM 88-3-3 as a multidrug resistance reversal agent, and concretely relates to a novel application of the THXM 88-3-3 in treating tumors with multidrug resistance and improving the sensitivity of tumor cells to anti-cancer drugs when the THXM 88-3-3 and the anti-cancer drugs are combined. The compound has the beneficial effects that the sensitivity of chemotherapeutic drugs to cancer cells is enhanced, the curative effect of the drugs is enhanced, and THXM 88-3-3 is expected to be developed into the tumor multidrug resistance reversal agent.

Description

technical field [0001] The invention relates to a method for extracting a new compound capable of killing multidrug-resistant tumor cells from active ingredients of traditional Chinese medicines, which belongs to the pharmacological activity of natural products and methods for separating and purifying natural raw materials. The present invention relates to the new use of compound THXM 88-3-3 as a multidrug resistance reversal agent, especially the new use of compound THXM 88-3-3 used in combination with antineoplastic drugs to treat multidrug resistant tumors and improve the sensitivity of tumor cells to antineoplastic drugs. Background technique [0002] Cancer has become one of the diseases that seriously endanger human health and is difficult to cure, and it is the second largest killer of human beings after cardiovascular disease. With the change of human living environment and living habits, the incidence of tumors is gradually increasing under the influence of adverse ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/366A61K36/58A61K45/06A61K31/704A61K31/136A61P35/00A61K131/00
CPCA61K31/366A61K36/58A61K45/06A61K31/704A61K31/136A61P35/00A61K2300/00
Inventor 马永钢杨文根郭晗
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com