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Nano biosensor for detecting hydrogen peroxide concentration, preparation method and application thereof

A technology of hydrogen peroxide concentration and biosensors, applied in the field of nano-biosensors and preparation, can solve the problems of not easy to stabilize mass production, low sensing signal strength, high equipment price, etc., and achieve low cost and high sensing signal strength , the effect of less detection steps

Active Publication Date: 2022-05-10
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the nano-biosensors prepared by the above-mentioned existing process methods have large differences in main components, limited sources of main components, high cost, complicated preparation methods and detection methods, high equipment prices, and it is not easy to achieve stable mass production. ; The chemical stability of the finished product is poor, the biocompatibility is poor, especially the sensing signal intensity is low, which limits its application in the biological field

Method used

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  • Nano biosensor for detecting hydrogen peroxide concentration, preparation method and application thereof
  • Nano biosensor for detecting hydrogen peroxide concentration, preparation method and application thereof
  • Nano biosensor for detecting hydrogen peroxide concentration, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The nano-biosensor using up-conversion luminescence to detect the concentration of hydrogen peroxide provided in this example is based on rare-earth up-conversion luminescence nanoparticles and histamine derivatives, specifically the rare-earth up-conversion luminescence nanoparticles NaYF synthesized by thermal coprecipitation method 4 : Yb / Er / Tm as the substrate, through the epoxidation of the oleic acid ligand on the surface of the substrate, a ring-opening addition reaction occurs with the electron-donating ligand with an amino group, and the electron-donating ligand is bonded to the up-conversion luminescent nanometer On the surface of the particle, under the irradiation of 365nm ultraviolet light, a layer of metallic silver is grown in situ on the surface of the up-conversion luminescent nanoparticles by means of photogenerated free electron reduction, forming up-conversion luminescent nanoparticles with a surface of metallic silver and a core of rare earth. Nano-b...

Embodiment 2

[0048] The nano-biosensor, preparation method and application provided in this example for detecting the concentration of hydrogen peroxide using up-conversion luminescence are basically the same as in Example 1, except that the preparation method uses rare earth up-conversion luminescence nanoparticles NaYF 4 : Yb / Tm as the basis, including the following steps:

[0049] (1) Preparation of electron-donating ligand: 0.2-0.4 g of histamine and 50-100 mg of NHS were ultrasonically dissolved in deionized water, magnetically stirred at a speed of 600-800 rpm, and kept stirring at room temperature for 12 hour, after fully mixing evenly, add 2mL serine aqueous solution (0.1~0.2g / mL) dropwise, continue stirring at room temperature for 12 hours, remove most of the solvent by rotary evaporator, and dilute to 2 with deionized water ~4mL, the first dispersion was obtained.

[0050] (2) Preparation of sensor precursor: prepare 10mL rare earth up-conversion luminescence nanoparticles NaYF ...

Embodiment 3

[0054]The nano-biosensor, preparation method and application provided in this example are basically the same as those in Examples 1 and 2, except that the preparation method uses rare earth upconversion luminescence Nanoparticles NaYbF 4 :Tm as the basis, including the following steps:

[0055] (1) Preparation of electron-donating ligand: 0.4 g of histamine and 100 mg of NHS were ultrasonically dissolved in deionized water, magnetically stirred at a speed of 800 rpm, and kept stirring at room temperature for 12 hours. , add 2 mL of serine aqueous solution (0.2 g / mL) dropwise, continue to stir at room temperature for 12 hours, remove most of the solvent by rotary evaporator, and dilute to 4 mL with deionized water to obtain the first dispersion.

[0056] (2) Preparation of sensor precursor: prepare 10 mL of rare earth up-conversion luminescent nanoparticles NaYbF 4 : Tm cyclohexane dispersion, prepare 50mL of 1,2-ethylene dichloride, mix with a volume ratio of 1:5, add 0.25g ...

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Abstract

The invention discloses a nano-biosensor for detecting the concentration of hydrogen peroxide, which is characterized in that it uses rare earth up-conversion luminescent nanoparticles synthesized by a thermal coprecipitation method as a substrate, and epoxidizes the oleic acid ligand on the surface of the substrate. , undergoes a ring-opening addition reaction with an electron-donating ligand with an amino group, and bonds the electron-donating ligand to the surface of the upconversion luminescent nanoparticle. A layer of simple metal silver is grown in situ on the surface of the converted luminescent nanoparticles to form a nanobiosensor with a core-shell structure whose surface is simple metal silver and whose core is rare earth upconversion luminescent nanoparticles as the matrix. The invention also discloses its preparation method and its application to realize the detection of hydrogen peroxide concentration at body surface and cell level. The nano biosensor provided by the invention has the advantages of good dispersion, stable structure, good biocompatibility, high sensing signal intensity and the like in water.

Description

technical field [0001] The invention relates to the technical field of manufacturing nano-biological materials, in particular to a nano-biosensor for detecting hydrogen peroxide concentration by using up-conversion luminescence, a preparation method and an application thereof. Background technique [0002] In recent years, biosensors have been considered in different fields as analytical alternatives to traditional sensors. A biosensor is an analytical device consisting of two basic parts: a biological receptor (antibody, DNA probe, or cell, etc.) for the specific detection of a substance by exploiting the specificity of biomolecular interactions; and a transducer or sensor , capable of interpreting and "converting" the biorecognition response generated by the receptor into quantifiable optical or electrical signals. The main factors that make these devices attractive analytical tools are their specificity, high sensitivity, responsiveness that shortens analysis time, abili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/85B82Y20/00B82Y30/00B82Y40/00A01N59/16A01P1/00A01P3/00G01N23/227
CPCC09K11/02C09K11/7773B82Y20/00B82Y30/00B82Y40/00A01N59/16G01N23/227G01N2223/084G01N2223/637
Inventor 孙丽宁王卓姜宏
Owner SHANGHAI UNIV
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