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Probe, method for detecting zearalenone and application

A technology of zearalenone and zearalenone, which is applied in the field of probes and detection of zearalenone, can solve the problems such as damage to the activity of monoclonal antibodies, obstacles to the suitability of nanomaterials, difficulty in replication, etc., to avoid Modification procedures or harsh conditions, preserved biological activity, highly specific effects

Active Publication Date: 2021-10-08
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the suitability of these nanomaterials is often hindered by some uncontrollable factors
The preparation of functional NMs usually involves harsh conditions, such as the need for toxic reagents, strong chemical reagents, high temperature, and high pressure, which are harmful to the environment and difficult to reproduce; secondly, the crossover of passive adsorption and covalent coupling between monoclonal antibodies and NMs The linkage method is not only easily affected by the isoelectric point, temperature, and ion concentration of the monoclonal antibody, but also the monoclonal antibody is also randomly and non-specifically immobilized on the surface of NMs, which may damage the activity of the monoclonal antibody.

Method used

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  • Probe, method for detecting zearalenone and application
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  • Probe, method for detecting zearalenone and application

Examples

Experimental program
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Embodiment 1

[0065] combine figure 1 and 2 , the present embodiment provides the signal carrier SAQDsRu and its preparation method. The signal carrier SAQDsRu is prepared based on the synthesis of quantum dot SAQDs by Staphylococcus aureus SA and doped with terpyridine ruthenium chloride hexahydrate; the particle size of the quantum dot SAQDs is 513 ~ 653nm, specifically 513.26-653.26nm, and the particle size of the signal carrier SAQDsRu is 519-650nm, specifically 519.06-645.94nm.

[0066] The preparation method of the signal carrier SAQDsRu comprises: first adding sodium selenite solid to the liquid medium containing Staphylococcus aureus, then adding cadmium chloride solid to obtain a quantum dot SAQDs solution synthesized based on Staphylococcus aureus SA, and finally, Add terpyridine ruthenium chloride hexahydrate solid to the quantum dot SAQDs solution synthesized based on Staphylococcus aureus SA to obtain a mixed solution, centrifuge the mixed solution, resuspend in water and inac...

Embodiment 2

[0076] Following the above technical scheme, this embodiment provides a probe and a preparation method, the probe includes a signal carrier and a monoclonal antibody adsorbed and bound to the signal carrier, the monoclonal antibody is a zearalenone monoclonal antibody, the signal carrier The carrier is the signal carrier SAQDsRu prepared in Example 1; the particle size of the signal carrier SAQDsRu is 519-650 nm; the concentration of the zearalenone monoclonal antibody is 1 mg / mL.

[0077] The method for preparing the probe comprises the following steps:

[0078] Step 1: adding sodium selenite solid to the liquid medium containing Staphylococcus aureus, and adding cadmium chloride solid to obtain a quantum dot SAQDs solution synthesized based on Staphylococcus aureus SA;

[0079] The final concentration of the sodium selenite is 4~6mM, the final concentration of the cadmium chloride is 1mM, the OD of the Staphylococcus aureus 600 The value is 1.0~2.6;

[0080] As a preferred...

Embodiment 3

[0088] combine Figure 7 , the present embodiment provides a method for detecting zearalenone, the method includes adding the probe (SAQDsRu-mAb) described in Example 2 into the sample to be detected, and then adding the zearalenone detection The test strip is inserted into the sample to be tested for detection. The probe is added to the actual sample to be detected to capture the target zearalenone (ZEN), and the bound SAQDsRu-mAb-ZEN immune complex moves to the test area of ​​the test paper by capillary action.

[0089] In this embodiment, the test strip for detecting zearalenone includes a liner, a nitrocellulose membrane is pasted on the liner, one end of the nitrocellulose membrane is covered with an absorbent pad, and the other end of the nitrocellulose membrane is sequentially covered. For the sample pad and the binding pad, the non-covered surface of the nitrocellulose membrane is provided with a detection line and a control line along the transverse direction, and th...

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Abstract

The invention discloses a probe, a method for detecting zearalenone and application. The probe comprises a signal carrier and a monoclonal antibody adsorbed and combined on the signal carrier, the monoclonal antibody is a zearalenone monoclonal antibody, and the particle size of the signal carrier SAQDsRu is 519-650 nm; the concentration of the zearalenone monoclonal antibody is 1 mg / mL. According to the invention, the novel dual-mode probe is prepared by biologically synthesizing quantum dots based on staphylococcus aureus and doping terpyridyl ruthenium chloride hexahydrate as a biological carrier labeled antibody in immunochromatography test strip detection for the first time, and the probe has high colorimetric and fluorescence signal intensity; the antibody can be specifically labeled by the aid of recognition functions of the staphylococcus aureus protein A, complicated modification procedures or severe conditions can be avoided, accordingly, the biological activity of the antibody can be obviously reserved, the lowest detection limits of the provided test strip on zearalenone are 0.008 ng / mL (colorimetric mode) and 0.0058 ng / mL (fluorescence mode) respectively, and the sensitivity is 13 times and 18 times that of the traditional colloidal gold test strip.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to a probe, a method for detecting zearalenone and an application thereof. Background technique [0002] Zearalenone (ZEN) is a class of non-steroidal estrogenic mycotoxins, widely present in corn, wheat, rice and other grains, feed and animal products, mainly causing damage to human endocrine and reproductive functions. It has reproductive toxicity, cytotoxicity, liver and kidney toxicity and immunotoxicity to animals and humans. Currently, commonly used detection methods for zearalenone include instrumental analysis and immunoassay. The instrumental analysis method has high sensitivity and can be accurately quantified, but the sample pretreatment is complicated and the detection equipment is expensive, so it is difficult to realize the on-site rapid detection technology. Immunoassay mainly includes enzyme-linked immunosorbent assay and immunochromatographic test strips. Althoug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/533G01N33/569
CPCG01N33/577G01N33/533G01N33/56961Y02A50/30
Inventor 王丽白菲儿补彤赵爽何坤益
Owner NORTHWEST A & F UNIV
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