Animal sciatic nerve ligation device

Abstract

The invention provides an animal sciatic nerve ligation device, and belongs to the field of neuropathic pain model preparation, wherein the animal sciatic nerve ligation device comprises a wire clamping box body, a power module box body and a U-shaped sliding rail; the wire clamping box body and the power module box body are placed on the U-shaped sliding rail and can be fixed on a sliding arm in a sliding mode; the wire clamping box body is connected with a first height adjuster; a first catgut single-end fixing clamp is connected with the first height adjuster; a sliding groove is formed in the power module box and slidably connected with an electric sliding rod placed in the sliding groove; the rear end of the electric sliding rod is connected with a spring, the rear end of the spring is connected with a tension sensor fixed to the rear end of the box body, and the front end of the electric sliding rod is connected with a second height adjuster; and a second catgut single-end fixing clamp is connected with the second height adjuster. According to the animal sciatic nerve ligation device, automatic tension detection, accurate tension control and automatic knotting are achieved through the tension sensor and the electric sliding rod, the position latitude of the equipment module is large, and the animal sciatic nerve ligation device is suitable for being used by different experimental animals.

Description

technical field [0001] The invention belongs to the field of neuropathic pain model preparation, in particular to an animal sciatic nerve ligation device. Background technique [0002] Neuropathic pain is pain caused by damage or disease of the somatosensory system, with sensory dysfunction as the main manifestation, such as spontaneous pain, hyperalgesia, allodynia, etc. The manifestations of the symptoms are diverse and the pathogenesis is still unclear. Chronic constriction injury of the sciatic nerve (CCI) model is a classic animal model for studying neuropathic pain. The preparation method of the CCI model is to bluntly separate the main part of the sciatic nerve of the experimental animal, and use 4-0 chrome catgut to surround the nerve trunk to make 3 or 4 light ligation rings, with a distance of 1mm, and the ligation strength is to cause mild calf muscle pain. Tremor response is appropriate. The sciatic nerve ligation method is easy to use, and the obtained neuropa...

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Problems solved by technology

[0003] However, the disadvantage of the CCI model is that accurate ligation strength is required in actual operation. If it is not handled properly, it may easily lead to model failure or sciatic nerve damage.

Due to human differences in operation, CCI is likely to cause different degrees of edema and necrosis in the rat sheath due to different ligation strengths. At the same time, it may affect the repeatability of the experiment and the observation of the whole experiment due to the large individual differences in animals. In addition, it is easy to cause differences in the sciatic nerve. For severe acute injury, such as acute crush injury and cutting injury, experimental animals are prone to acral autophagy, have a high rate of self-mutilation, and can also cause local tissue infection, so that there is a certain gap between animal models and clinical neuropathic pain.

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