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Preparation method of natural photosensitizer hypocrellin nano bactericidal emulsion

A technology of hypocrellin and nano-sterilization, applied in food ingredients as emulsifiers, food ingredients as antimicrobial preservation, food science, etc., can solve the problems of poor water solubility of photosensitizers, limit the application of photosensitizers, poor stability, etc. Achieve the effects of reducing production costs, expanding the scope of application, and improving solubility and usability

Pending Publication Date: 2021-09-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor water solubility and poor stability of photosensitizers under high temperature and light, this greatly limits the application of photosensitizers in photodynamic inactivation.

Method used

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  • Preparation method of natural photosensitizer hypocrellin nano bactericidal emulsion
  • Preparation method of natural photosensitizer hypocrellin nano bactericidal emulsion
  • Preparation method of natural photosensitizer hypocrellin nano bactericidal emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of photosensitizer: take bamboo yellow as raw material, grind it into powder, take 160g and place it in a Soxhlet extractor, add 500mL of acetone, heat with 75mL, carry out heat reflux, and circulate for 10h; Rotary steam for 30min to recover acetone; put in a beaker, add 200mL petroleum ether, heat with 80mL, stir while heating until boiling, carry out 30min, after cooling, use Buchner funnel suction filtration, repeat 3 times, get the initial product; Add 100mL of toluene and petroleum ether to the beaker of the initial product, recrystallize in 50mL for 30min, and dry in 70mL for 24h to obtain hypocrellin.

[0029] Preparation of primary emulsion: take 0.1g hypocrellin powder, add medium-chain triglycerides to 10g, take 1.8g lecithin, add deionized water to 90g; mix the oil phase and water phase, at 300r / min speed, temperature 10min at 55mL.

[0030] Preparation of nanoemulsion: The primary emulsion was sheared at a speed of 1300r / min for 25 minutes in a...

Embodiment 2

[0032] Preparation of photosensitizer: take bamboo yellow as raw material, grind it into powder, take 160g and place it in a Soxhlet extractor, add 500mL of acetone, heat with 85mL, carry out heat reflux, and circulate for 10h; Rotary steam for 30min to recover acetone; put in a beaker, add 200mL petroleum ether, heat with 80mL, stir while heating until boiling, carry out 30min, after cooling, use Buchner funnel suction filtration, repeat 3 times, get the initial product; Add 100mL of toluene and petroleum ether to the beaker of the initial product, recrystallize in 50mL for 30min, and dry in 70mL for 24h to obtain hypocrellin.

[0033] Preparation of primary emulsion: take 0.1g hypocrellin powder, add medium-chain triglycerides to 10g, take 1.8g lecithin, add deionized water to 90g; mix the oil phase and water phase, at 300r / min speed, temperature 10min at 55mL.

[0034] Preparation of nanoemulsion: The primary emulsion was sheared at a speed of 1300r / min for 25 minutes in a...

Embodiment 3

[0036]Preparation of photosensitizer: take bamboo yellow as raw material, grind it into powder, take 160g and place it in a Soxhlet extractor, add 500mL of acetone, heat with 85mL, carry out heat reflux, and circulate for 10h; Rotary steam for 30min to recover acetone; put in a beaker, add 200mL petroleum ether, heat with 80mL, stir while heating until boiling, carry out 30min, after cooling, use Buchner funnel suction filtration, repeat 3 times, get the initial product; Add 100mL of toluene and petroleum ether to the beaker of the initial product, recrystallize in 50mL for 30min, and dry in 70mL for 24h to obtain hypocrellin.

[0037] Preparation of primary emulsion: take 0.2g hypocrellin powder, add medium chain triglycerides to 10g, take 1.8g lecithin, add deionized water to 90g; mix the oil phase and water phase, at 300r / min speed, temperature 10min at 55°C.

[0038] Preparation of nanoemulsion: The primary emulsion was sheared at a speed of 1300r / min for 25 minutes in a ...

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Abstract

The invention relates to a preparation method of a natural photosensitizer hypocrellin nano bactericidal emulsion, and belongs to the technical field of food sterilization. The preparation method comprises the following steps of firstly, dissolving a photosensitizer in medium chain triglyceride (MCT) to obtain an oil phase, dissolving an emulsifier in deionized water to obtain a water phase, uniformly mixing and stirring the oil phase and the water phase to prepare a primary emulsion, and then obtaining the natural photosensitizer hypocrellin nano bactericidal emulsion through preparation by adopting a high-speed shearing-high-pressure homogenizing technology. According to the preparation method of the natural photosensitizer hypocrellin nano bactericidal emulsion, the adopted materials are all food-grade raw materials, are non-toxic and harmless, are safe and reliable, have no side effects on human bodies and are low in cost, the prepared nano bactericidal emulsion is easy to store, the sterilization rate of the nano bactericidal emulsion on golden yellow glucose coccus can reach 90%, and the nano bactericidal emulsion can be widely applied to the field of food sterilization and disinfection.

Description

technical field [0001] The invention belongs to the technical field of food sterilization, and in particular relates to the production technology of a natural photosensitizer hypocretin nano-sterilizing emulsion. Background technique [0002] With the continuous development of human food production and processing technology, various foods continue to appear, but with it comes the continuous discovery of various food-borne bacteria. Food-borne bacteria are mainly composed of diarrhea-causing Escherichia coli, Staphylococcus aureus, Salmonella, Listeria monocytogenes, Vibrio parahaemolyticus and other bacteria. It is widely spread and can cause various diseases. At present, for the inhibition of food-borne bacteria, the existing technologies mainly use high temperature, high pressure, ultraviolet light irradiation, antibiotics, metal ions and other methods. On the one hand, these technologies are subject to harsh conditions of use, and on the other hand, they will cause dama...

Claims

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Application Information

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IPC IPC(8): A23L3/3499A23L29/10A23L29/00
CPCA23L3/3499A23L29/10A23L29/04A23V2002/00A23V2200/10A23V2200/222A23V2250/208A23V2250/1842A23V2250/1944
Inventor 张春玲方强胜李睿袁媛庄红
Owner JILIN UNIV
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