Microfluidic colon cell research model and method for researching influence mechanism of novel coronavirus on colon cells

Abstract

The invention belongs to the technical field of in-vitro simulation analysis, and particularly relates to a microfluidic colon cell research model for researching the influence mechanism of novel coronavirus on colon cells, the microfluidic colon cell research model comprises more than one culture mechanism, one culture mechanism is selected as a control group, and other culture mechanisms are selected as experimental groups; each culture mechanism comprises a culture tank and more than one collection tank; and the inlet of the collection tank is connected with the outlet of the culture tank through a liquid channel valve. The beneficial effects are that a stimulation device is simple in structure and low in cost; a A549 cell layer and a human colon goblet cell HT29-CL.16E or HUVEC cell layer are respectively arranged, so that the structure of colon capillary vessels is effectively simulated; the rhythmic stretching of a vacuum pump is used for simulating colon contraction and relaxation caused by human respiration; the simulation device is simple to operate; an exchange cavity is in a streamline spindle shape, and fluid flowing non-uniformity is prevented by considering fluid mechanics factors.

Description

technical field [0001] The invention belongs to the technical field of in vitro simulation analysis, and in particular relates to a microfluidic colonic cell research model and method for research on the mechanism of the influence of novel coronavirus on colonic cells. Background technique [0002] The outbreak of the new coronavirus in 2019 has aroused widespread concern from the international community. On January 30, 2020, the World Health Organization has declared that the outbreak has constituted a "public health emergency of international concern". For human beings, diseases always go hand in hand, and infectious diseases are the enemy of human beings. In the 20th century alone, smallpox claimed the lives of 300 million people. The Spanish virus that broke out from April 1918 to May 1919 caused 25 million deaths. - 40 million people died. In 2009, the H1N1 influenza outbreak in Mexico and California lasted for more than a year, causing 284,500 deaths worldwide. In 2014...

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Problems solved by technology

[0005] Although animal models consider the impact of air pollutants on the body as a whole, they are costly, time-consuming, and difficult to conduct quantitative research in vivo; in addition, due to differences between species, animal models cannot represent human physiological and pathological characteristics to some extent , causing limitations in the application of animal models

[0006] The in vitro cell model uses cultured human lung cell lines for quantitative and high-throughput analysis, but due to the inability to realize the in vivo cell environment, the lack of information interaction between various cells, mechanical force, etc., it is difficult to simulate the results in vivo. The actual situation

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