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Marine self-flocculating bacterium, halophilic nitrogen assimilation microbiome driven and developed by marine self-flocculating bacterium, and construction method and application of halophilic nitrogen assimilation microbiome

A construction method and nitrogen assimilation technology, applied in the field of microbial and environmental treatment and remediation, can solve the problems of long sludge domestication time, unclear metabolic characteristics, complicated regulation methods, etc., and achieve good organic matter and phosphorus removal ability and high removal efficiency. , the effect of wide application prospects

Active Publication Date: 2021-09-17
SHANDONG UNIV
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Problems solved by technology

However, the inventors found that the sludge used in this method has various microbial communities, unclear metabolic characteristics, complex control methods, long sludge domestication time, and slow system startup.

Method used

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  • Marine self-flocculating bacterium, halophilic nitrogen assimilation microbiome driven and developed by marine self-flocculating bacterium, and construction method and application of halophilic nitrogen assimilation microbiome
  • Marine self-flocculating bacterium, halophilic nitrogen assimilation microbiome driven and developed by marine self-flocculating bacterium, and construction method and application of halophilic nitrogen assimilation microbiome
  • Marine self-flocculating bacterium, halophilic nitrogen assimilation microbiome driven and developed by marine self-flocculating bacterium, and construction method and application of halophilic nitrogen assimilation microbiome

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preparation example Construction

[0076] d) preparation of organic fertilizers;

[0077] e) improvement of soil fertility;

[0078] f) Bioremediation of saline-alkali land;

[0079] g) Water eutrophication control;

[0080] h) Remediation of water pollution;

[0081] i) Greenhouse gas emission reduction and carbon neutrality;

[0082] Wherein, in said b), nitrogen is metabolized based on assimilation;

[0083] In said c), the wastewater includes high-salt sewage, mariculture wastewater, industrial saline sewage, and seawater flushing wastewater;

[0084] In said g), the water body includes fresh water and sea water, preferably sea water.

[0085] In yet another specific embodiment of the present invention, a method for the integration and resource transformation of nutrients in a high-salt environment is provided, the method includes: applying the above-mentioned seawater psychrobacter (Psychrobacteraquimaris) A4N01, microbial Bacteria and / or halo-nitrogen assimilating microbiome.

[0086] In yet anothe...

Embodiment 1

[0089] The screening and cultivation method of seawater psychrophilic bacillus (Psychrobacter aquimaris) A4N01, the steps are as follows:

[0090] The marine sediments taken from the Yellow Sea (122°48′E, 35°59′N) were inoculated into seawater LB medium, shaken and cultivated in a shaker at 25°C and 200r / min for 24h, and then The supernatant was separated by streaking on a seawater LB solid plate.

[0091] The single colony formed on the plate was inoculated into seawater LB medium, and cultured in a shaker at 25°C and 200r / min for 24 hours, and the microorganisms with flocculation ability were observed and screened.

[0092] Inoculate the above-screened strains with flocculation ability in ammonia nitrogen medium, investigate their ammonia nitrogen and total nitrogen removal capabilities, and screen strains with high ammonia nitrogen removal efficiency, no nitrite and nitrate production, and no loss of total nitrogen in the system, that is It is Psychrobacter aquimaris A4N01...

Embodiment 2

[0095]The method for measuring the physicochemical properties of nitrogen metabolism of Psychrobacter aquimaris A4N01 strain is as follows

[0096] (1) Seawater psychrobacter (Psychrobacter aquimaris) A4N01 was inoculated in seawater LB medium, shaken and cultivated in a shaker at 25°C and 200r / min for 24h to prepare an activated bacterial liquid. ① Take the obtained activated Psychrobacter aquimaris (Psychrobacter aquimaris) A4N01 and centrifuge at 4000rpm for 10min; ②After removing the supernatant, use sterilized physiological saline (0.85% NaCl) to resuspend and wash the bacteria; centrifuge at 4000rpm for 10min. ③ After the bacterial cells were resuspended in sterilized normal saline, they were respectively inoculated in the medium containing ammonia nitrogen, nitrous nitrogen, and urea as nitrogen sources according to the inoculum amount of 10%. utilization and transformation.

[0097] The ammonia nitrogen, nitrite nitrogen, and urea medium in the above method are prepar...

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Abstract

The invention provides a marine self-flocculating bacterium, a halophilic nitrogen assimilation microbiome driven and developed by the marine self-flocculating bacterium, and a construction method and application of the halophilic nitrogen assimilation microbiome, and belongs to the technical field of microorganisms and environmental governance and repair. The Psychrobacter aquimaris A4N01 is screened from marine sediments, ammonia nitrogen can be assimilated to synthesize organic nitrogen, organic matter and phosphorus can be synchronously converted, the halophilic nitrogen assimilation microbiome developed by the Psychrobacter aquimaris A4N01 has good nutrient substance conversion and removal capacity and settling performance, and the salinity tolerance is wide; and the halophilic nitrogen assimilation microbiome can be used in the directions of high-salt sewage treatment, saline-alkali soil fertility improvement and the like, realizes recovery of nutrient elements such as nitrogen and phosphorus in a high-salt environment, is simple to operate, low in price and environment-friendly, and thus, has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganism and environmental governance and restoration, and specifically relates to a marine self-flocculation bacterium and a salt-nitrogen assimilation microbiome driven by it and its construction method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The transformation of nutrients in a high-salt environment is easily inhibited by salinity, and there are problems such as difficult treatment of high-salt sewage, difficult transformation of substances in saline-alkali land, and easy loss of nutrients. However, since the high-salt sewage contains a large amount of nitrogen and phos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01
CPCC12N1/20C12N1/36C02F3/34C12N1/205C02F2101/16C02F2103/005C02F2103/08C02F3/1263C12R2001/01
Inventor 周维芝张梦汝韩飞刘喆
Owner SHANDONG UNIV
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