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Octapeptide and application thereof in preparation of medicine and health care product for improving memory

A technology for improving memory and health care products, applied in applications, medical preparations containing active ingredients, peptides, etc., can solve the problems of being easily degraded by proteases or polypeptide enzymes in the body, low bioavailability, and limited applications, etc., and achieve non-toxicity Effects of side effects, improvement of memory impairment, good safety and practicality

Active Publication Date: 2021-09-17
RONGCHENG TAIXIANG FOOD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, peptide drugs also have disadvantages such as poor stability, short half-life, low bioavailability, and easy degradation by proteases or peptide enzymes in vivo, which greatly limits their application.

Method used

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  • Octapeptide and application thereof in preparation of medicine and health care product for improving memory
  • Octapeptide and application thereof in preparation of medicine and health care product for improving memory
  • Octapeptide and application thereof in preparation of medicine and health care product for improving memory

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The synthesis of polypeptide WCPFSRSF by solid-phase synthesis includes the following steps: swelling and washing the dichloro resin, removing the Fmoc protecting group, adding the constituent amino acids of the polypeptide for condensation reaction, and repeating the process of removal-protection-condensation until all amino acids are connected . Purified by reverse-phase high-performance liquid chromatography to obtain pure polypeptide (>95%).

Embodiment 2

[0044] Effect of octapeptide on glutamate-induced neuronal cell survival. Such as figure 1 As shown, the cell survival rates of 50 μM and 100 μM WCPFSRSF treatment were 69.03±1.21% and 87.00±3.21%, respectively. The drug of positive control: the survival rate of cells treated with cerebrolysin (0.5 mg / mL) was 67.58±1.79%.

[0045] The test results show that the octapeptide WCPFSRSF has a better neuroprotective effect.

Embodiment 3

[0047] Determination of lactate dehydrogenase (lactate dehydrogenase, LDH)

[0048] SH-SY5Y cells were divided into 2×10 5Cells / mL were inoculated in a transparent 96-well plate, and culture medium was added. Six wells were set up for each experimental group, and blank wells and control wells were set up at the same time. After 24 hours, the medium was discarded, the model group was replaced with a medium containing 37.5mM glutamic acid, and the sample group was replaced with a medium containing both glutamic acid and samples, while cerebrolysin (0.5mg / mL) was used as a positive control , the blank group and the control group were replaced with fresh medium, and cultured for 24 hours. The culture was terminated before the detection, the supernatant was taken, and the lactate dehydrogenase kit was used for detection according to the instructions.

[0049] Such as figure 2 As shown, the test results showed that the LDH release rate of the glutamic acid treatment group was 1...

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PUM

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Abstract

The invention discloses octapeptide and application thereof in preparation of a medicine and a health-care product for improving memory. The amino acid sequence of the polypeptide is as follows: Trp-Cys-Pro-Phe-Ser-Arg-Ser-Phe (WCPFSRSF). The octapeptide provided by the invention can eliminate sleep deprivation induced mouse dysmnesia, and can significantly inhibit the excitatory toxicity and the oxidative stress of nerve cells. The polypeptide disclosed by the invention can be independently applied to health-care products for improving the memory, also can be compounded with other active ingredients capable of improving the memory and also can be prepared into microcapsules to improve the gastrointestinal digestion stability, the bioavailability and the shelf life of the polypeptide, and therefore, the polypeptide can be more favorably applied to the food industry and the field of health-care products.

Description

technical field [0001] The invention relates to an octapeptide and its application in preparing memory-improving medicines and health care products. Background technique [0002] Glutamate is the major amino acid excitatory neurotransmitter in the brain. When glutamate accumulates too much in the synaptic cleft, the high extracellular concentration of glutamate leads to overstimulation of glutamate receptors, which in turn leads to calcium overload and leads to oxidative stress and apoptosis, which ultimately leads to cell damage and neuron loss, and even trigger neurodegenerative diseases. Conversely, elevated calcium ion flux further exacerbates oxidative stress and accelerates neuronal death, a phenomenon known as excitotoxicity. The excitotoxicity of glutamate is the main cause of neuronal death, which is closely related to a variety of neurological diseases such as Alzheimer's disease and ischemic stroke. Therefore, the neuronal cell model of glutamate damage is ofte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61K45/06A61P25/28A23L33/18A23P10/30
CPCC07K7/06A61K45/06A61P25/28A23L33/18A23P10/30A61K38/00A23V2002/00A23V2200/322A23V2250/55A23V2250/1868A23V2250/1846A23V2250/18A23V2250/214A23V2250/1842A23V2250/712A23V2250/71A23V2250/54252A23V2200/224A23V2250/5114A23V2250/5432A23V2300/10A23V2300/48A23V2250/5026A23V2250/5118A23V2250/511
Inventor 赵谋明张琪杨青王金梅位正鹏苏国万曾海英沈源庆沈萍萍
Owner RONGCHENG TAIXIANG FOOD
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