Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of EphB4 as target in screening of drugs or models for improving insulin sensitivity

An insulin sensitivity and insulin sensitization technology, applied in the application field of the new target EphB4, can solve the problems of lack of insulin sensitizers, weight gain and cardiovascular risk, improve glucose tolerance and clearance, and improve insulin resistance. , the effect of enhancing sensitivity

Active Publication Date: 2021-09-17
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TZD drugs have been withdrawn from the market or restricted due to side effects such as weight gain and cardiovascular risk.
Therefore, there is no better insulin sensitizer in the currently applied drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of EphB4 as target in screening of drugs or models for improving insulin sensitivity
  • Application of EphB4 as target in screening of drugs or models for improving insulin sensitivity
  • Application of EphB4 as target in screening of drugs or models for improving insulin sensitivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Research methods and technical routes

[0033] Cell models and animal models were combined, and insulin-sensitized animal models of HEK293T cells, HepG2 cells, primary hepatocytes, C57BL / 6J mice, db / db mice, and EphB4 liver-specific knockout mice ( EphB4 LKO) mice were used as research objects, and corresponding cell or tissue samples were collected for corresponding research.

[0034] The research methods are based on real-time quantitative PCR, Western blot, glucose tolerance and insulin tolerance of mice.

[0035] 2. Experimental materials

[0036] Transfection reagent Lipo3000 was purchased from Invitrogene.

[0037] InsR, EphB4, Akt and pAkt antibodies were purchased from Cell Signaling Technology, the article numbers are: # 3025, # 14960, # 4685 and #4060, use a ratio of 1:1000-2000 for western blotting and 1:100 for protein immunoprecipitation.

[0038] The adenovirus overexpressing EphB4, the lentivirus knocking down EphB4 expression and the correspond...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of EphB4 as a target in screening of drugs or models for improving insulin sensitivity, belongs to the technical field of protein and gene engineering, and particularly discloses application of the EphB4 as a target in screening and preparation of biological agents or drugs for improving the insulin sensitivity. The invention also discloses application of the EphB4 in preparation of an insulin-sensitized mouse model. According to the application, on the basis of insulin signal regulation, a protein EphB4 capable of interacting with an insulin receptor (InsR) is found, the protein EphB4 can interact with the InsR, and insulin stimulation can promote interaction between the protein EphB4 and the InsR, so that a certain basis is provided for insulin resistance under the condition of hyperinsulinemia. The degradation of the InsR can be promoted under the condition of overexpression of EphB4; and by inhibiting the EphB4, the sensitivity to insulin can be enhanced, and the insulin resistance can be improved.

Description

technical field [0001] The invention relates to the technical field of protein and genetic engineering, in particular to the application of a new target EphB4 for preventing and treating insulin resistance and related diseases. Background technique [0002] In my country, with changes in eating habits and lifestyle factors, the incidence of diabetes is on the rise. As of 2010, the incidence of diabetes in the Chinese adult population reached 11.6%, and the proportion of pre-diabetic population was as high as 50.1%. By 2015, the number of adult diabetic patients in China was as high as 109.6 million, which is the country with the largest number of adult diabetic patients in the world. At present, diabetes has become a public health issue of widespread concern. If it cannot be intervened in time, type 2 diabetes may induce diabetes-related metabolic syndrome such as cardiovascular disease, kidney disease, eye disease, etc., seriously affecting the patient's body health, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P3/10A61P5/50
CPCA61K45/00A61P3/10A61P5/50G01N33/566G01N2500/00A01K2227/105A01K2267/0362A01K2217/075C12N2710/10343C12N2740/16043C12N15/907A01K67/0275C07K14/715G01N33/6872A01K2217/054C12N2310/20A61K49/0008C12N9/22C12N15/11C12N2800/80
Inventor 李平平柳星峰崔冰王凯陈婧文侯少聪孔丽娟姜茜马春晓
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com