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Cell preservation solution as well as preparation method and application thereof

A technology for preserving liquid and cells, applied in the biological field

Active Publication Date: 2021-09-14
广州朗坤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are formaldehyde-free preservation solutions on the market, but there is no preservation solution that can simultaneously meet the four screening methods of thin-layer liquid-based cytology detection, HPV detection, immunocytochemical staining and reduced blue histiocytic staining.

Method used

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  • Cell preservation solution as well as preparation method and application thereof
  • Cell preservation solution as well as preparation method and application thereof
  • Cell preservation solution as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 A kind of cell preservation solution

[0062] A cell preservation solution, its components and contents are shown in Table 1.

[0063] Components and content of cell preservation solution in Table 1 Example 1

[0064] components content Disodium phosphate 4g Sodium dihydrogen phosphate 8g potassium chloride 5g Sodium chloride 5g calcium chloride 2.5g magnesium chloride 1.25g ethanol 200mL Acetic acid 150mL Trypsin 10g glucose 8g double distilled water Quantitatively make up 1000mL

[0065] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0066] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 600mL double distilled water, stir and dissolve to obtain solution a;

[0067] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chloride to solution a, stir and dissolve to ob...

Embodiment 2

[0071] Embodiment 2 A kind of cell preservation solution

[0072] A cell preservation solution, its components and contents are shown in Table 2.

[0073] Component and content of cell preservation solution in table 2 embodiment 2

[0074] components content Disodium phosphate 5g Sodium dihydrogen phosphate 10g potassium chloride 6g Sodium chloride 6g calcium chloride 3g magnesium chloride 1.5g ethanol 150mL Acetic acid 200mL Trypsin 12g glucose 4g fructose 4g ascorbic acid 2g double distilled water Quantitatively make up 1000mL

[0075] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0076] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 600mL double distilled water, stir and dissolve to obtain solution a;

[0077] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chlori...

Embodiment 3

[0081] Embodiment 3 A kind of cell preservation solution

[0082] A cell preservation solution, its components and contents are shown in Table 3.

[0083] Components and content of cell preservation solution in table 3 embodiment 3

[0084]

[0085]

[0086] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0087] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 800mL double distilled water, stir and dissolve to obtain solution a;

[0088] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chloride to solution a, stir and dissolve to obtain solution b;

[0089] (3) Add trypsin to solution b, stir and dissolve to obtain solution c;

[0090] (4) Add glucose, fructose and ascorbic acid to solution c, stir and dissolve to obtain solution d;

[0091] (5) Add ethanol and acetic acid to the solution d, stir and mix evenly, adjust the pH to 6.5 with NaOH, and distill the...

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Abstract

The invention belongs to the technical field of biology, and discloses a cell preservation solution as well as a preparation method and application thereof. The cell preservation solution comprises alcohol, acid, buffer salt, an ionic strength maintaining agent, an antioxidant and proteolytic enzyme. According to the cell preservation solution, samples of multiple mucus and blood cells can be well treated, and the staining effect of thin-layer liquid-based cytology detection is improved; however, the proteolytic enzyme can influence the staining effect of reductive blue tissue cells, the influence of the proteolytic enzyme on the staining of the reductive blue tissue cells is reduced by adding the antioxidant, and meanwhile, the accuracy and the stability of immune cell chemical staining and reductive blue tissue cell staining are improved; and moreover, the cell preservation solution has no influence on the accuracy and stability of HPV detection after preserving a cell sample. The cell preservation solution can meet requirements of four screening methods including thin-layer liquid-based cytology detection, HPV detection, immunocytochemical staining and vat blue tissue cell staining at the same time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell preservation solution and its preparation method and application. Background technique [0002] Currently, cervical cancer is one of the most common malignant tumors among female cancers. Early diagnosis of cervical cancer and precancerous lesions is the key to improving the cure rate of cervical cancer and the survival rate of patients. Thin-Cytologic Test (TCT) is currently the most widely used clinical method for screening cervical cancer . Thin-layer liquid-based cytology detection is a discipline that diagnoses clinical diseases by observing changes in cell morphology and structure. For decades, thin-layer liquid-based cytology detection has become the main method for large-scale cervical cancer screening and follow-up observation of high-risk groups, which has greatly reduced the incidence of advanced cervical cancer. The smear made by the traditional cyto...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12Q1/6851C12Q1/70G01N1/30G01N33/569
CPCA01N1/0226A01N1/0221A01N1/021C12Q1/708C12Q1/6851G01N33/56966G01N1/30G01N2469/10C12Q2531/113C12Q2563/107
Inventor 邹刚军夏婷谭芷晴卢智俊
Owner 广州朗坤生物科技有限公司
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