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Preparation method and application of bifidobacterium animalis F1-7 and Euphausia superba oil composition for improving atherosclerosis lipid metabolism

A technology for animal bifidobacteria and atherosclerosis, applied in the field of food microbiology, can solve the lack of effect verification and mechanism revealing of improving atherosclerosis animal bifidobacteria, reasonable ratio and combination effect of probiotics and krill oil And the mechanism has not been effectively verified and explored

Pending Publication Date: 2021-09-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Atherosclerosis-modifying substances combining probiotics and krill oil have not been reported so far
At the same time, the reasonable ratio and combination effect and mechanism of effective probiotics and krill oil have not been effectively verified and explored
Lack of efficacy verification and mechanism revealing of Bifidobacterium animalis F1-7 and krill oil composition for improving atherosclerosis

Method used

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  • Preparation method and application of bifidobacterium animalis F1-7 and Euphausia superba oil composition for improving atherosclerosis lipid metabolism
  • Preparation method and application of bifidobacterium animalis F1-7 and Euphausia superba oil composition for improving atherosclerosis lipid metabolism
  • Preparation method and application of bifidobacterium animalis F1-7 and Euphausia superba oil composition for improving atherosclerosis lipid metabolism

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] like figure 1 As shown, the preparation method of improving atherosclerosis Bifidobacterium animalis F1-7 and krill oil composition provided by the embodiment of the present invention comprises the following steps:

[0025] S101, culturing Bifidobacterium animalis F1-7: inoculating Bifidobacterium animalis F1-7 into the enrichment MRS medium of a fermenter for cultivation to obtain a fermentation broth;

[0026] S102, drying of Bifidobacterium animalis F1-7: pre-freeze the Bifidobacterium animalis F1-7, pre-freezing treatment, main drying treatment and analytical drying treatment in order to complete the preparation of Bifidobacterium freeze-dried products;

[0027] S103, preparation and packaging of the composition of Bifidobacterium animalis F1-7 and krill oil: mixing Bifidobacterium animalis F1-7 and krill oil to prepare a composition of Bifidobacterium animalis F1-7 and krill oil.

[0028] S104, adding the composition of Bifidobacterium animalis F1-7 and krill oil ...

example 1

[0030] Example 1. The composition of Bifidobacterium animalis F1-7 and krill oil has a good effect of improving atherosclerosis

[0031] The experimental results are shown in Figure 2. The aortic wall of the atherosclerosis model mice was thickened, hardened and easily damaged, and there were calcified plaques in the blood vessels. The plaque on the inner wall of the aorta in the Mycobacterium F1-7 and krill oil composition group was significantly reduced, and the wall of the aorta was firm and elastic. Using software to analyze the plaque coverage area proved that the three intervention groups can effectively improve atherosclerosis caused by high-fat diet.

[0032] Analyzing the HE staining results of mouse liver cases, it was found that the liver cells of mice in the AS group were swollen, the cytoplasm was loose, and lipid droplet vacuoles of different sizes appeared ( image 3 ). Bifidobacterium animalis F1-7, krill oil, Bifidobacterium animalis F1-7 and krill oil compo...

example 2

[0035] Example 2. The lipid-lowering mechanism of Bifidobacterium animalis F1-7 and krill oil

[0036] Determination of expression levels of key genes of lipid metabolism in atherosclerotic mice Image 6 As shown, the probiotic intervention can effectively down-regulate the expression of FXR in the intestinal tract of model mice (p<0.05), the effect of krill oil intervention is better than that of Bifidobacterium animalis F1-7 intervention group, and the mixed intervention of probiotics and active substances can down-regulate Intestinal FXR expression, but no significant difference with the other two groups. After intervening with bifidobacterium F1-7, krill oil and the mixture of the two in mice with atherosclerosis, compared with the model group, these three intervention groups could down-regulate the expression of FGF15 in the mouse intestine, and each There were no differences between groups. The expression of CYP7A1, a key protein in bile acid metabolism, was determined...

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Abstract

The invention belongs to the technical field of food microorganisms and biological medicines, and discloses a preparation method and an application effect of a composition for reducing lipid and reliving atherosclerosis. A bifidobacterium animalis F1-7 and Euphausia superba oil composition consists of bifidobacterium animalis F1-7 and Euphausia superba oil; and the composition is prepared by mixing 10<8> CFU / mL of bifidobacterium animalis F1-7 and 6 mg of Euphausia superba oil according to a proportion of 1:5 to 1:10. The probiotic Euphausia superba oil composition provided by the invention can inhibit atherosclerotic lipid accumulation, and has very wide application prospects. The invention also relates to application of dropping pills, tablets, granules and capsules prepared from the bifidobacterium animalis F1-7 and Euphausia superba oil composition to medicines, health care foods, special medical formula foods and special diet foods.

Description

technical field [0001] The invention belongs to the technical field of food microbes, and in particular relates to a preparation method and application of solid oral preparations such as capsules, granules, tablets, etc. for reducing lipid, anti-inflammation, and improving atherosclerosis. Effect. Background technique [0002] Cardiovascular diseases (CVDs) are one of the leading causes of death worldwide and are closely related to atherosclerosis. Atherosclerosis is a pathological change characterized by the accumulation of blood lipids, especially cholesterol, in the intima of large and medium-sized arteries. Lipid accumulation is a key factor in the occurrence and development of atherosclerosis. In the past, the treatment of atherosclerosis and other related diseases mostly focused on lowering lipids, and its therapeutic efficacy was also uneven. According to recent studies, the imbalance of intestinal flora has an important impact on the pathogenesis and progression of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/115A23L33/135A61K35/745A61P9/10A61K35/612
CPCA23L33/115A23L33/135A61K35/745A61K35/612A61P9/10A23V2002/00A61K2300/00A23V2200/326A23V2250/18A23V2250/5432A23V2250/6406A23V2250/1886A23V2250/192A23V2250/082A23V2250/5112A23V2250/5114A23V2250/51088
Inventor 刘同杰梁曦张兰威张喆吕优优易华西公丕民
Owner OCEAN UNIV OF CHINA
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