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Method for improving rooting rate of taxus media tissue culture seedlings

A technology of Taxus chinensis and tissue culture seedlings, which is applied in the field of plant propagation, can solve the problems of limited test materials, limited popularization and application, and unsuitability for industrialized seedling cultivation, so as to save space and materials, and has broad application prospects. Prominent growth-promoting effect

Pending Publication Date: 2021-08-31
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional cutting method is time-consuming and laborious in the cultivation process, and requires a large breeding space, which is not suitable for industrial seedling cultivation; in addition, if the genetic transformation or breeding of Taxus Mandia is carried out, it may happen that the test materials are extremely Limited, the traditional cutting method is a waste of material, which is not conducive to the study of seedling and rooting of genetic transformation system
[0004] The breeding method of tissue culture in a bottle is controllable, does not need frequent water management, and saves materials, and is suitable for rapid propagation of rare plant materials; It is extremely difficult to take root in internal tissue culture, the rooting rate is usually less than 30%, and the growth rate is slow, which limits the related promotion and application

Method used

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  • Method for improving rooting rate of taxus media tissue culture seedlings
  • Method for improving rooting rate of taxus media tissue culture seedlings
  • Method for improving rooting rate of taxus media tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 Open tissue culture antibacterial condition research

[0057] 1. Natural drop test

[0058] Prepare media in an open environment without autoclaving:

[0059]Add 8g / L agar to sugar-free 1 / 2MS liquid medium (Table 1), boil until transparent, adjust pH to 5.8-6.0, add bacteriostat after cooling down to about 50°C, fully stir the medium, and quantitatively distribute Put it into a clean tissue culture bottle, expose it to the air for 1 hour, seal it and place it in an incubator at 25°C for cultivation.

[0060] Bacteriostatic agents are: sodium hypochlorite solution, 70% dysonium wettable powder, 50% carbendazim wettable powder, potassium sorbate and 75% chlorothalonil wettable powder; each bacteriostatic agent is set at 0.05, 0.1 , 0.25, 0.5, 1.0, 1.5g / L6 concentrations, plus blank control (CK) for a total of 31 treatments, each treatment set 5 samples, repeated 3 times, according to the pollution situation, judge the antibacterial effect, if there is antib...

Embodiment 2

[0083] The screening of embodiment 2 culture medium agar concentration

[0084] Add agar powder (2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6g / L, 7 concentrations in total) into the sugar-free 1 / 2MS medium liquid, stir evenly with a glass rod, gradually cook until transparent, adjust When the pH is 5.8-6.0, without high temperature and high pressure sterilization, the boiled culture medium of each concentration is divided into two parts, one part is added with 0.08g / L mancozeb, the other part is not added with antibacterial agent, and then poured into In a clean tissue culture bottle, five replicate samples were processed for each treatment, cooled for 2 hours, and the solidification state and hardness of the medium were observed and recorded.

[0085] The result is as shown in table 5, when the agar concentration is at 2.5-3g / L, the medium will have flow and sticky wall phenomenon, can not play the effect of supporting the explant; When the concentration is at 5-6g / L, the medium is hard, ...

Embodiment 3

[0088] Embodiment 3 tissue culture rooting experiment

[0089] Prepare media in an open environment without autoclaving:

[0090] Add 4g / L agar to the sugar-free 1 / 2MS liquid medium, boil until transparent, adjust the pH to 5.8-6.0, cool down to about 50°C, add 0.08g / L mancozeb, fully stir the medium, and quantify Pack into tissue culture bottles, seal and set aside.

[0091] Take 1-2 year-old robust branches of Taxus chinensis, brush off the dust on the surface with a soft brush, cut off 3-5cm long stems with axillary buds, and remove 1 / 3-1 / 2 needles on the stems from the base , as explants.

[0092] Put the explants into a beaker, drop a few drops of detergent, stir and soak for 10 minutes, rinse with running water for 1 hour; then completely soak in alcohol with a mass fraction of 75% for 30 seconds, rinse with sterile water for 5 times; then completely soak in mass fraction Sterilize in 0.1% mercuric chloride for 10 minutes, then rinse with sterile water 8 times. After...

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Abstract

The invention discloses a method for improving a rooting rate of taxus media tissue culture seedlings. The method comprises the following steps: (1) selection of explants: selecting 1-2-year-old semi-lignified robust branches of taxus media, cutting 3-5cm-long stem segments with axillary buds, and removing base needle leaves to obtain explants; (2) treatment of explants: after cleaning and disinfecting the explants, soaking the explants in an ABT1 solution, an NAA solution or an IBA solution for 5-30 min; and (3) rooting culture: inoculating the explants soaked in step (2) into a sugar-free 1 / 2 MS agar culture medium containing 0.08 g / L of mancozeb and carrying out light and shade alternate culture at 25+ / -2 DEG C, wherein an illumination intensity is 2,000-2,800Lx and an illumination duration is 12 h / d. The method provided is simple and controllable to operate, has a remarkable effect of promoting growth of a taxus media root system, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of plant propagation, and more specifically relates to a method for improving the rooting rate of Taxus chinensis tissue culture seedlings. Background technique [0002] Taxus Mandia is a natural hybrid of Taxus chinensis and Taxus chinensis; among the 11 species of Taxus chinensis in the world, the content of taxol in the roots, stems and leaves of Taxus Mandia is relatively high, and its tree shape is beautiful, and it is suitable for all seasons. Evergreen, strong cold resistance, broad market prospects. [0003] At present, the research on the propagation of Taxus Mandia is mainly based on the traditional cutting method outside the bottle, and the rooting rate can reach more than 90%. However, the traditional cutting method is time-consuming and laborious in the cultivation process, and requires a large breeding space, which is not suitable for industrial seedling cultivation; in addition, if the geneti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨艳芳丰美静陈段芬邱德有蒋路园陈雅琦
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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