Nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme and preparation and application thereof

A technology of graphene quantum dots and nitrogen doping, applied in the field of nanozymes, can solve the problems of time-consuming reaction, unfriendly reaction to the environment, and low yield, and achieve the effect of low cost and selective detection

Active Publication Date: 2021-08-24
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this preparation method needs to use a large amount of strong oxidizing acid, the reaction is not friendly to the environment, and the reaction is time-consuming and the yield is low.

Method used

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  • Nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme and preparation and application thereof
  • Nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme and preparation and application thereof
  • Nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme and preparation and application thereof

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Experimental program
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Embodiment 1

[0044] 1. Hydrothermal synthesis of nitrogen-doped graphene quantum dots

[0045] 1. After hydrothermal synthesis in an aqueous solution of aminopyrene for a certain period of time, the resulting solution is purified by dialysis and further freeze-dried to obtain a nitrogen-doped graphene quantum dot solid. Wherein, the aminopyrene concentration is 0.5 mg / mL, the hydrothermal reaction temperature is 200° C., and the hydrothermal reaction time is 10 h. After the reaction product was fully dialyzed through a dialysis bag with a molecular weight cut-off of 500 Da, the solution in the dialysis bag was fully dialyzed again through a dialysis bag with a molecular weight cut-off of 3500 Da, and the solution outside the dialysis bag was the nitrogen-doped graphene quantum dot solution.

[0046] 2. Characterization and detection

[0047] The nitrogen-doped graphene quantum dots (N-GQDs) prepared in step 1 were characterized by transmission electron microscopy, atomic force microscopy,...

Embodiment 2

[0066] 1. Hydrothermal synthesis of nitrogen-doped graphene quantum dots

[0067] After hydrothermal synthesis in an aqueous solution of aminopyrene for a certain period of time, the obtained solution is purified by dialysis and further freeze-dried to obtain a nitrogen-doped graphene quantum dot solid. Wherein, the aminopyrene concentration is 2.0 mg / mL, the hydrothermal reaction temperature is 180° C., and the hydrothermal reaction time is 6 h. After the reaction product was fully dialyzed through a dialysis bag with a molecular weight cut-off of 500 Da, the solution in the dialysis bag was fully dialyzed again through a dialysis bag with a molecular weight cut-off of 3500 Da, and the solution outside the dialysis bag was the nitrogen-doped graphene quantum dot solution.

[0068] 2. Preparation of nitrogen-doped graphene quantum dots-iron ion nanocomposites

[0069] Mix iron ions with nitrogen-doped graphene quantum dots 3 μmoL: 1g to prepare nitrogen-doped graphene quantum...

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Abstract

The invention discloses a nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme and a preparation method and application thereof, and belongs to the technical field of nano-enzymes. The preparation method of the nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme comprises the following steps: synthesizing nitrogen-doped graphene quantum dots by using 1-aminopyrene as a carbon source and a nitrogen source and H2O as a reaction medium through a one-step hydrothermal method, and compounding the nitrogen-doped graphene quantum dots with Fe < 3 + > to obtain the nitrogen-doped graphene quantum dot-iron ion composite nano-enzyme. The nano-composite has the activity of pseudo-peroxidase. The nitrogen-doped graphene quantum dot-iron ion composite nano enzyme can be used as pseudo-peroxidase to distinguish hydroquinone and isomers thereof, and selective colorimetric detection of hydroquinone is realized. The preparation method is simple, and the synthesized nano-enzyme is high in activity.

Description

technical field [0001] The invention relates to the technical field of nanozymes, in particular to a nitrogen-doped graphene quantum dot-iron ion composite nanozyme, a preparation method thereof and an application in detecting hydroquinone. Background technique [0002] As biocatalysts, natural enzymes can efficiently catalyze various biochemical reactions. However, natural enzymes are expensive to purify, easily hydrolyzed by proteases, and easily denatured at extreme pH and temperature. Therefore, the synthesis of "artificial enzymes" that mimic the functions of natural enzymes has attracted widespread attention. With the deepening of research, "nanozyme" has become a research hotspot in the field of simulated enzymes in recent years. Nanozymes refer to nanomaterials that can exhibit enzymatic properties at the nanoscale. Similar to natural enzymes, nanozymes can efficiently oxidize enzyme substrates under mild physiological conditions, produce the same reaction product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J27/24B01J23/745G01N21/78G01N21/31C09K11/65
CPCB01J27/24B01J23/745G01N21/78G01N21/31C09K11/0883C09K11/65Y02E60/50
Inventor 奚凤娜杨罗星晏菲麻露予
Owner ZHEJIANG SCI-TECH UNIV
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