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CeO2 nanorod-based urine glucose test paper and preparation method thereof

A technology of nanorods and test paper, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. Effects in simple steps

Pending Publication Date: 2021-08-17
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In summary, CeO 2 Nanomaterials have been widely used in many fields, but so far no technicians have been found to apply them to the research and development of test paper. If we can develop a low-cost, simple process, easy industrial production and obvious color comparison Test paper for testing will undoubtedly have a beneficial impact on further broadening its market application scope

Method used

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  • CeO2 nanorod-based urine glucose test paper and preparation method thereof
  • CeO2 nanorod-based urine glucose test paper and preparation method thereof

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Embodiment 1

[0029] Weigh 1g Ce(NO 3 ) 3 ·6H 2 O and 12g NaOH were mixed, stirred at room temperature for 1 h to obtain a white suspension, and the white suspension was transferred into an autoclave for a hydrothermal reaction in an oven at 110°C for 9 h, the supernatant was poured off, and the precipitate was washed until neutral, then in At room temperature, use a freeze dryer (FD-1D-80, Beijing Boyikang Experimental Instruments) to dry the sample to constant weight to obtain CeO 2 Nanorod samples; the CeO 2 Nanorods were mixed with 3,3,5,5-tetramethylbenzidine (TMB) and glucose oxidase at a mass ratio of 1:1:1 to prepare a solution, which was put into blank test paper (qualitative filter paper, Hangzhou Special Paper Industry Co., Ltd.) after soaking for a certain period of time, take out the test paper and dry it to get the CeO-based 2 A new type of urine glucose test strip based on nanorods.

[0030] For the CeO obtained in this embodiment 2 Nanorod samples, microscopically morp...

Embodiment 2

[0032] Weigh 1g Ce(NO 3 ) 3 ·6H 2 O and 12g NaOH were mixed, stirred at room temperature for 1 h to obtain a white suspension, and the white suspension was transferred to a high-pressure reactor, and hydrothermally reacted in an oven at 130°C for 9 h, the supernatant was poured off, and the precipitate was washed until neutral. Then use a freeze dryer (FD-1D-80, Beijing Boyikang Experimental Instrument) to dry the sample to a constant weight at room temperature to obtain CeO 2 nanorod samples, the CeO 2 Nanorods, TMB, and glucose oxidase were mixed and stirred at a mass ratio of 1:0.5:1 to obtain a solution, which was soaked in a blank test paper for a certain period of time, and then the test paper was taken out and dried to obtain a solution based on CeO 2 A new type of urine glucose test strip based on nanorods.

[0033] Microcosmic morphological analysis is carried out on it through a transmission electron microscope, and the obtained sample presents a uniform rod-like...

Embodiment 3

[0037] Weigh 1.2g Ce(NO 3 ) 3 ·6H 2 Mix O and 12g KOH, stir at room temperature for 1 h to obtain a white suspension, transfer the white suspension into an autoclave, and conduct a hydrothermal reaction in an oven at 130°C for 9 h, pour off the supernatant, and wash the precipitate until neutral. Then use a freeze dryer (FD-1D-80, Beijing Boyikang Experimental Instrument) to dry the sample to a constant weight at room temperature to obtain CeO 2 Nanorod samples. CeO 2 Nanorods are mixed with 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and glucose oxidase at a mass ratio of 1:0.5:1 to prepare a solution by stirring the solvent, and put in After soaking the test paper for a certain period of time, take out the test paper and dry it to obtain the CeO-based 2 Nano-rod urine glucose test strip.

[0038] Microscopic morphological analysis is carried out on it by a transmission electron microscope, and the obtained sample presents a uniform rod-like structure wi...

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Abstract

The invention discloses CeO2 nanorod-based urine glucose test paper and a preparation method thereof. The preparation method comprises the steps: firstly, mixing cerium salt and inorganic base, and then fully stirring the mixture, forming white suspension liquid, and then moving thewhite suspension liquid into a high-pressure reaction kettle, and carrying out a hydrothermal reaction in a drying oven; removing the supernatant, washing the precipitate to be neutral, and drying the precipitate to obtain a CeO2 nanorod; mixing the CeO2 nanorod with a color developing agent and glucose oxidase in proportion to prepare a solution, immersing blank test paper into the solution, taking out the test paper, and drying the test paper to obtain the CeO2 nanorod-based urine glucose test paper. The test paper can develop after being placed in an environment containing glucose with different concentrations and taken out, the color is light in a low-concentration stage, the color of the test paper is gradually deepened in a gradient manner along with the deepening of the glucose concentration, the different stages are obviously distinguished, and the detection sensitivity is high; according to the invention, the limitation of the application field of the CeO2 nano material is broken through, and the market application range of the CeO2 nano material is further widened.

Description

technical field [0001] The invention belongs to the technical field of medicinal detection, in particular to a CeO-based 2 Nano rod urine sugar test paper and preparation method thereof. Background technique [0002] Nanozyme (also known as nanomimetic enzyme) is an extremely important branch in the field of artificial enzymes. In recent decades, researchers have controlled the size and surface functional groups of nanomaterials to make them have different natural enzyme activities. Compared with natural enzymes and other simulated enzymes, nanozymes have other unique properties of nanomaterials such as optics, electricity, and magnetism in addition to catalytic activity, which makes them have dual or even multiple functions. In addition, the large specific surface area and rich surface chemical properties of nanomaterials make nanozymes easy to chemically modify and connect various biomolecules, which facilitates the construction of bioprobes and sensors. Nanozymes based...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 刘勇陈莹范曲立尹维翰昝佳慧况培培黄逾东
Owner NANJING UNIV OF POSTS & TELECOMM
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