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Probiotic composition for preventing type II diabetes as well as preparation method and application thereof

A technology for probiotics and compositions is applied in the field of probiotic composition preparation, which can solve problems such as adverse reactions in the gastrointestinal tract, hypoglycemia, etc., and achieve the effects of good effect, high number of viable bacteria and strong stability

Active Publication Date: 2021-08-17
贵州酵德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many drugs have been developed to control blood sugar or inhibit the production and absorption of glucose by injecting insulin, these drugs have a certain degree of side effects, such as sulfonylureas have the risk of causing acute severe hypoglycemia; lactic acidosis is also the use of biguanides Potential side effects caused by metformin; the use of metformin may also cause gastrointestinal adverse reactions, etc.

Method used

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  • Probiotic composition for preventing type II diabetes as well as preparation method and application thereof
  • Probiotic composition for preventing type II diabetes as well as preparation method and application thereof
  • Probiotic composition for preventing type II diabetes as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A preparation method of a probiotic composition for preventing type II diabetes

[0034] The preparation method of the probiotic composition in the present embodiment comprises the following steps carried out in sequence:

[0035] 1) Preparation of Lactobacillus plantarum N3117 bacterial powder

[0036] Take 10 parts of casein peptone, 12 parts of beef extract, 4 parts of yeast extract, 22 parts of glucose, 6 parts of sodium acetate, 1.5 parts of diamine citrate, 1 part of Tween-80, K 2 HPO 4 2.5 parts, MgSO 4 ·7H 2 O 0.15 parts, MnSO 4 ·7H 2 O 0.04 parts and 1000 parts of distilled water, mix well to prepare MRS liquid medium.

[0037] Get 0.2kg of Lactobacillus plantarum N3117 and inoculate it in the MRS liquid medium of 10kg (the inoculum size of Lactobacillus plantarum N3117 is 2% of the weight of the MRS liquid medium, marked as FL1), after 40°C anaerobic proliferation and cultivation for 52h, the obtained The cultured bacterial solution was centr...

Embodiment 2～6

[0043] The preparation method of embodiment 2~6 probiotic composition

[0044] Examples 2 to 6 are respectively a preparation method of a probiotic composition, and their steps are basically the same as in Example 1, the difference is only in the amount of raw materials, see Table 1 for details:

[0045] Table 1 The amount of raw materials in the probiotic composition and a list of parameters

[0046]

[0047]

[0048] The content of the other parts of Examples 2-6 is the same as that of Example 1.

Embodiment 7

[0049] Example 7 Preventive effect of probiotic composition on blood sugar in type II diabetic rats

[0050] 40 clean healthy male SD rats were randomly divided into normal control group (ND group, n=10), diabetes model group (DM group, n=10), preventive high-dose group (Pre-High group, n=10 ) and the low-dose prevention group (Pre-Low group, n=10), the ND group was fed with common feed, and the remaining three groups were fed with high-fat feed and mixed with high and low doses of bacterial powder for prevention. After feeding for 4 weeks, the model group and the composition intervention group received once or twice STZ (30mg / kg) to induce diabetes by intraperitoneal injection, and the fasting blood glucose and postprandial 2h blood glucose data of the rats in the diabetes model group and the composition intervention group were analyzed, Fasting blood glucose level ≥ 7.8mmol / L and 2h postprandial blood glucose level ≥ 11.1mmol / L were the successful criteria for modeling. Rat...

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Abstract

The invention provides a preparation method of a probiotic composition for preventing type II diabetes. The preparation method comprises the following steps: A, inoculating lactobacillus plantarum N3117 into an MRS liquid culture medium, and carrying out anaerobic enrichment culture at 35-40 DEG C for 44-52 hours to obtain a fermentation bacterial solution; B, centrifugally separating the fermented bacterial solution, and collecting precipitates to obtain bacterial sludge; C, adding a freeze-drying protective additive solution into the bacterial sludge, performing vacuum freeze-drying after emulsification, and performing crushing and sieving to obtain lactobacillus plantarum N3117 bacterial powder; and D, mixing the lactobacillus plantarum N3117 bacterial powder with pumpkin powder, Chinese yam powder, inulin and citric acid powder to obtain the probiotic composition. The probiotic composition adopted by the invention has the basic characteristics and biological functions of lactobacillus plantarum N3117, and compared with lactobacillus plantarum N3117 bacterial powder, the probiotic composition has the advantages of reasonable formula ratio, full play of the functionality of each component, high viable count, strong stability, easy preservation and the like.

Description

technical field [0001] The technical field of preparation of the probiotic composition of the present invention, in particular relates to a probiotic composition for preventing type II diabetes and its preparation method and application. Background technique [0002] In recent years, with the aging of my country's social population and changes in people's dietary preferences and lifestyles, diabetes has changed from a rare disease to a common and frequently-occurring disease today. Diabetes is placing a heavy burden on global health. In the next 25 years, diabetes will become the main disability and killer threatening human health. According to the report of the International Diabetes Federation, there were 382 million diabetes cases in the world in 2013, and it is expected to reach 592 million by 2035, 80% of which occurred in developing countries. According to statistics, the prevalence of diabetes in my country rose to 11.2% from 2015 to 2017, and the incidence is still...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61K35/747A61P1/00A61P3/10A61K31/194A61K31/733
CPCA61K36/42A61K36/8945A61K35/747A61K31/733A61K31/194A61P1/00A61P3/10A61K2236/19A61K2300/00
Inventor 赵宝华边艳青李璐刘尧尧张博玮
Owner 贵州酵德生物科技有限公司
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