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Application of lactobacillus rhamnosus in product for preventing periodontal disease

A technology of Lactobacillus rhamnosus and periodontal disease, applied in the directions of Lactobacillus rhamnosus, application, dairy products, etc., can solve the problems of drug toxicity, drug side effects, affecting the treatment effect, etc., and achieve the effect of preventing periodontal disease

Pending Publication Date: 2021-08-10
SYNBIOTECH BIOTECHNOLOGY YANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the existing basic treatment methods for periodontal disease often have parts that the machine cannot reach, thus affecting the treatment effect, and although the drug treatment has the problems of drug side effects and drug toxicity

Method used

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  • Application of lactobacillus rhamnosus in product for preventing periodontal disease
  • Application of lactobacillus rhamnosus in product for preventing periodontal disease
  • Application of lactobacillus rhamnosus in product for preventing periodontal disease

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] (1) Preparation of lactic acid bacteria fermentation broth supernatant

[0042] Take 5 g of the prepared Lactobacillus rhamnosus LRH09 lactic acid bacteria fermentation liquid and put it in a centrifuge tube, centrifuge at 3000r / min for 10min, collect the supernatant and filter it with a 0.22μm filter membrane for sterilization before use.

[0043] (2) Preparation of BHI solid medium

[0044] Weigh 3.7g of BHI medium powder and 3.7g of agar plus distilled water to make up to 100mL, autoclave at 121°C for 20min, wait until the temperature is cooled to about 50°C, pour it into a plate for later use.

[0045] (3) Preparation of TSA medium

[0046] Weigh 1.5g of tryptone, 0.5g of plant peptone, 3g of sodium chloride, 1.5g of agar, add distilled water to make up to 100mL, keep stirring and heating to boil for 1min, put them in test tubes at 121°C, autoclave for 20min, and cool for later use .

[0047] (4) Antibacterial experiment of lactic acid bacteria fermentation broth...

Embodiment 1

[0060] 1) Deployment: Execute according to the following formula:

[0061] Whole milk powder 8g; whey protein powder 1g; yeast extract 0.5g; isomalt-oligosaccharide syrup 4g; fructo-oligosaccharide 1g; galacto-oligosaccharide 2g;

[0062] 2) Sterilization: 88°C, 30min.

[0063] 3) Cooling: cooling to 42°C

[0064] 4) Inoculation: Inoculate Lactobacillus rhamnosus LRH09, so that the number of original bacteria is 1.0×10 4 cfu / ml.

[0065] 5) Fermentation: Fermentation temperature is 34° C., time is 18 hours.

[0066] 6) Filling.

[0067] 7) The application of the Lactobacillus rhamnosus LRH09 lactic acid bacteria fermented liquid prepared in step 6) in the preparation of food and daily chemical products, the total amount of which is 5% of the total weight of the final product.

Embodiment 2

[0069] 1) Deployment: Execute according to the following formula:

[0070] Whole milk powder 12g; whey protein powder 1g; yeast extract 0.5g; isomalto-oligosaccharide syrup 4g; fructo-oligosaccharide 2g;

[0071] 2) Sterilization: 90°C, 20min.

[0072] 3) Cooling: cooling to 42°C

[0073] 4) Inoculation: Inoculate Lactobacillus rhamnosus LRH09, so that the number of original bacteria is 1.0×10 5 cfu / ml.

[0074] 5) Fermentation: Fermentation temperature is 40° C., time is 15 hours.

[0075] 6) Filling.

[0076] 7) The application of the Lactobacillus rhamnosus LRH09 lactic acid bacteria fermented liquid prepared in step 6) in the preparation of food and daily chemical products, the total amount of which is 5% of the total weight of the final product.

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Abstract

The invention relates to an application of lactobacillus rhamnosus LRH09 in preparation of a product for preventing periodontal diseases. The lactobacillus rhamnosus LRH09 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus rhamnosus LRH09 is CGMCC NO.19423. The invention provides a new application of the lactobacillus rhamnosus LRH09, and the lactobacillus fermentation liquor prepared by adopting the lactobacillus rhamnosus and the food or daily chemical products taking the lactobacillus fermentation liquor as an effective component can be used for improving the dysbacteriosis in the oral cavity so as to maintain the immune homeostasis of the organism for a long time; therefore, the traditional Chinese medicine composition has good prevention and improvement effects on periodontal diseases, and can be used as an auxiliary treatment means for periodontal diseases.

Description

technical field [0001] The invention relates to the application of Lactobacillus rhamnosus LRH09 in the preparation of products capable of preventing periodontal disease, and belongs to the technical field of biomedicine processing. Background technique [0002] Periodontal disease is a disease caused by dental plaque infection. More than 80% of adults in my country suffer from different degrees of periodontal disease. Patients with periodontal disease will have increased dental calculus, and even gingival recession , Alveolar bone lesions will also occur in different courses of disease. The etiology of periodontal disease is complex, periodontal microecological imbalance bacterial infection is the main way to cause periodontal disease, studies have found that there are at least two kinds of anaerobic bacteria in the oral cavity of patients with periodontal disease, such as Fusobacterium nucleatum, Actinobacillus, Straight Campylobacter, etc. are the main bacteria that cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23L21/10A23L33/135A23G4/12A61K8/99A61Q11/00A61P31/04A61P1/02C12R1/225
CPCC12N1/20A23C9/1234A23L21/10A23L33/135A23G4/123A61K8/99A61Q11/00A61P1/02A61P31/04A23V2002/00A61K2800/85A23V2400/175A23V2200/312
Inventor 许慈雪杨锁华姜益军徐德丰金世嘉王霞
Owner SYNBIOTECH BIOTECHNOLOGY YANGZHOU CO LTD
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