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Lactobacillus fermentum ZS40 having prophylactic effect on colitis

A technology for colitis and Lactobacillus fermentum, applied in the field of microorganisms, can solve problems such as side effects, and achieve the effects of enhancing flavor, improving ulcerative colitis, and improving food safety

Active Publication Date: 2021-08-10
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common drugs for the treatment of colitis include sulfasalazine, aminosalicylic acid, glucocorticoids, etc., but continuous use of drugs will cause certain side effects to the human body. Colitis has become a hot spot of scientific research in recent years

Method used

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  • Lactobacillus fermentum ZS40 having prophylactic effect on colitis
  • Lactobacillus fermentum ZS40 having prophylactic effect on colitis
  • Lactobacillus fermentum ZS40 having prophylactic effect on colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] 1. Isolation and identification of Lactobacillus fermentum ZS40

[0090] 1. Isolation of Lactobacillus fermentum ZS40

[0091] Lactobacillus fermentum (Lactobacillus fermentum) ZS40 was isolated from traditionally fermented yak yogurt in the Balikesukesk grassland of Zhaosu County.

[0092] Draw 1mL sample (traditionally fermented yak yoghurt in Balikesukesk Grassland, Zhaosu County) by aseptic operation, and perform 10-fold gradient dilution with sterile normal saline to 10 -6 . Select an appropriate gradient dilution solution, pipette 100 μL each into the MRS solid plate medium with a sterile pipette tip, incubate at 37°C for 48 hours, observe and record the colony morphology. Pick colonies of different shapes on the plate for streak separation, and after culturing at 37°C for 48 hours, pick single colonies of different shapes on the plate again for streak separation, and repeat this 2 to 3 times until a pure single colony with the same shape is obtained. colony. ...

Embodiment 2

[0151] A fermented sausage comprises the following preparation steps:

[0152] (1) mince 100 parts by weight of fresh pork from which fascia and tendons have been removed, and stir to make pork stuffing;

[0153] (2) adding auxiliary materials to the pork stuffing obtained in step (1) and marinating at 4°C;

[0154] (3) Inoculate 0.2 parts by weight of starter into the pork stuffing of step (2), stir evenly, and prepare sausage;

[0155] (4) Place the sausage obtained in step (3) at 25° C. and RH95% to ferment for 3 days;

[0156] (5) Fermenting the sausage in step (4) at 15°C and RH72% for 11 days;

[0157] (6) vacuum packaging to obtain fermented sausage;

[0158] Described starter is Lactobacillus fermentum (Lactobacillus fermentum) ZS40 starter;

[0159] The preparation method of described Lactobacillus fermentum (Lactobacillus fermentum) ZS40 starter is to inoculate Lactobacillus fermentum (Lactobacillus fermentum) ZS40 in MRS liquid culture medium, 37 DEG C cultivate...

Embodiment 3

[0162] It is basically the same as Embodiment 2, the difference is only in:

[0163] The starter is Staphylococcus xylosus (Staphylococcus xylosus) ATCC29971 starter.

[0164] The preparation method of the Staphylococcus xylosus (Staphylococcus xylosus) ATCC29971 starter is to inoculate Staphylococcus xylosus (Staphylococcus xylosus) ATCC29971 in MRS liquid medium, and activate it by cultivating at 37°C for 16 hours to obtain Staphylococcus xylosus (Staphylococcus xylosus) xylosus) ATCC29971 is 1×10 6 cfu / mL activation culture; the above-mentioned Staphylococcus xylosus (Staphylococcus xylosus) ATCC29971 was 1 × 10 6 The cfu / mL activated culture was inoculated at 2vol% in MRS liquid medium, cultured for 16h, centrifuged at 4000r / min for 15min at 4°C, collected and washed with sterile water for 3 times, and centrifuged at 4000r / min for 20min to obtain Staphylococcus xylosus (Staphylococcus xylosus) ATCC29971 bacterial classification; Add the Staphylococcus xylosus (Staphyloco...

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Abstract

The invention discloses lactobacillus fermentum ZS40, which has a prevention effect on colitis, and the preservation number of the lactobacillus fermentum ZS40 is CGMCC NO.18226. The strain has high gastric acid resistance, and the survival rate of the strain treated by artificial gastric juice with the pH value of 3.0 for 3 h reaches 79.32%; and in 0.30% cholate, the growth efficiency of the strain reaches 15.31% of that of cholate-free culture. The research finds that the strain not only can inhibit mouse colon shortening, colon injury, intestinal wall thickening and other phenomena, but also can increase T-SOD and CAT activity, reduce MPO and MDA contents, regulate balance imbalance of pro-inflammatory cytokines and anti-inflammatory cytokines, and inhibit activation of NF-kappa B and MAPK signal channels so as to better relieve inflammation. In conclusion, the strain can effectively relieve mouse symptoms of colitis and can be used for preventing and treating colitis.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Lactobacillus fermentum ZS40 which has a preventive effect on colitis. Background technique [0002] Colitis is a chronic non-specific inflammatory bowel disease, the main symptoms include abdominal pain, diarrhea, blood in the stool, weight loss and so on. The severity of the disease varies, and most of them are recurrent, which seriously affects the quality of life of the patient, and may further deteriorate into colon cancer. Colitis occurs all over the world, but the incidence rate varies among people of different races, regions, ages, and genders. combined effect. In recent years, epidemiological data have shown that the incidence and prevalence of colitis are on the rise, which may be due to changes in people's lifestyles, diet, and work and rest. Colitis has become one of the most common diseases of the digestive system in China. Common drugs for the treatment of co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P29/00A23L13/60A23L13/40A23L33/135C12R1/225
CPCA61K35/747A61P1/00A61P29/00A23L13/65A23L13/45A23L33/135A23V2002/00A23V2400/143A23V2200/15A23V2200/3204A23V2250/61Y02A40/90
Inventor 赵欣
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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