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Method for improving in-vitro maturation and development rate of porcine oocytes by adopting agomelatine

A technology of agomelatine and oocytes, applied in germ cells, animal cells, vertebrate cells, etc., can solve the problems of insufficient antioxidant defense, reduce the in vitro development rate of oocytes and embryos, and improve pig The effect of oocyte quality and in vitro development ability of PA embryos, improvement of blastocyst development ability, and reduction of expression level

Pending Publication Date: 2021-08-06
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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AI Technical Summary

Problems solved by technology

[0007] In the process of in vitro culture of oocytes and embryos in the prior art, the cells are in a relatively high-oxygen environment, which often leads to oxidative stress due to insufficient antioxidant defense, so that the cells accumulate a large amount of reactive oxygen species (ROS) during development. Thereby reducing the in vitro development rate of oocytes and embryos, the present invention provides a method for improving the in vitro maturation and development rate of pig oocytes using agomelatine, specifically using the melatonin receptor agonist agomelatine A method for improving the in vitro maturation and development rate of porcine oocytes, which significantly improves porcine cumulus cell expansion, oocyte in vitro maturation (In Vitro Maturation, IVM), oocyte development-related gene expression, and parthenogenetic embryo development ability

Method used

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  • Method for improving in-vitro maturation and development rate of porcine oocytes by adopting agomelatine
  • Method for improving in-vitro maturation and development rate of porcine oocytes by adopting agomelatine
  • Method for improving in-vitro maturation and development rate of porcine oocytes by adopting agomelatine

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Experimental program
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Effect test

Embodiment 1

[0032] The method for improving the in vitro maturation and development rate of porcine oocytes by using agomelatine comprises the following steps:

[0033] 1) Prepare oocyte maturation medium:

[0034] A culture medium: 80% volume of TCM199, 10% volume of porcine follicular fluid, 10% volume of fetal bovine serum, 0.1g / L cysteine, 0.075g / L penicillin, 0.05g / L streptomycin, 10ng / mL insulin-like growth factor, 50ng / mL epidermal growth factor, 2.2g / L NaHCO 3 , 10IU / mL pregnant horse serum gonadotropin, 10IU / mL human chorionic gonadotropin, 10 - 11 The agomelatine of mol / L concentration;

[0035] B culture fluid: 80% volume of TCM199, 10% volume of porcine follicular fluid, 10% volume of fetal bovine serum, 0.1g / L cysteine, 0.075g / L penicillin, 0.05g / L streptomycin, 10ng / mL insulin-like growth factor, 50ng / mL epidermal growth factor, 2.2g / L NaHCO 3 、10 -11 The agomelatine of mol / L concentration;

[0036] 2) Prepare electroactivation solution: 0.3mmol / L mannitol, 0.1mmol / ...

Embodiment 2

[0042] Adopt agomelatine to improve the method of porcine oocyte maturation and development rate in vitro, compared with embodiment 1, the consumption of agomelatine in step 1) is 1 * 10 -10 mol / L, others are the same as in Example 1.

Embodiment 3

[0044] Adopt agomelatine to improve the method of porcine oocyte maturation and development rate in vitro, compared with embodiment 1, the consumption of agomelatine in step 1) is 1 * 10 -9 mol / L, others are the same as in Example 1.

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Abstract

The invention discloses a method for improving the in-vitro maturation and development rate of porcine oocytes by adopting agomelatine. Particularly, the method for improving the in-vitro maturation and development rate of the porcine oocytes by adopting a melatonin receptor stimulant agomelatine is adopted, so that porcine cumulus cell expansion, oocyte in vitro maturation (IVM), oocyte development related gene expression and parthenogenetic embryo developmental ability are obviously improved.

Description

【Technical field】 [0001] The invention relates to the technical field of in vitro maturation and culture of oocytes, and relates to a method for improving the in vitro maturation and development rate of pig oocytes by using agomelatine, in particular to improving pig oocytes by using agomelatine, a melatonin receptor agonist A method for in vitro maturation and developmental rate of cells. 【Background technique】 [0002] my country is a big pork industry country. Pigs are important agricultural economic animals, and pigs are similar to humans in terms of anatomy, physiology, genetic background and disease characteristics. They are considered to be the first choice for the source of human xenogeneic organs. Therefore, pigs play an important role in biomedical research. is an important model animal. The effective combination of in vitro production technology and gene editing technology not only promotes the development and application of embryo engineering, but also drives the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075C12N13/00
CPCC12N5/0609C12N13/00C12N2501/825C12N2500/32C12N2501/105C12N2501/11C12N2501/31C12N2529/00C12N2500/12C12N2500/30C12N2500/33
Inventor 吕玲燕吴柱月汪燕玲肖正中孙如玉廖晓光张家庆王献伟关志惠廖海洪
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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