Hepatitis C virus antibody detection kit and application thereof

A hepatitis C virus and antibody detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of false positives and false negatives, and achieve the effects of strong specificity, easy operation, and high sensitivity

Inactive Publication Date: 2021-07-30
中山生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the current chemiluminescent immunoassay for detecting hepatitis C antibody is prone to false positive or false negative.

Method used

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  • Hepatitis C virus antibody detection kit and application thereof
  • Hepatitis C virus antibody detection kit and application thereof
  • Hepatitis C virus antibody detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This embodiment prepared a hepatitis C virus antibody detection kit, including the following components:

[0044] (1) Coated plate coated with hepatitis C virus antigen

[0045] After the hepatitis C virus antigen is purified by affinity chromatography, a band is detected by polyacrylamide gel electrophoresis with a molecular weight of about 40KD and a purity of ≥98%. The purified antigen is stored at -20°C or below .

[0046] Coating: After diluting hepatitis C virus antigen with coating buffer (the volume ratio of hepatitis C virus antigen and coating buffer is 1:1000), add 100 μL / well into the wells of the microwell plate Adsorbed at 37°C for 48 hours, and then dried; blocking: use phosphate buffer solution containing 0.8% tryptone, 5% sucrose, and 0.02% Proclin300 as blocking solution, add 200 μL / well to the wells of the microplate, and Seal at 37°C for 16 hours; dry and seal the plate: discard the blocking solution, seal after drying, and obtain a coated plate co...

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Abstract

The invention discloses a hepatitis C virus antibody detection kit and application thereof. The hepatitis C virus antibody detection kit comprises a hepatitis C virus antigen coated plate, an enzyme conjugate and a chemiluminescent substrate. The prepared kit is used for detecting the antibody and has the effects of high specificity, good repeatability and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis and detection, and in particular relates to a hepatitis C virus antibody detection kit and application thereof. Background technique [0002] Hepatitis C virus is caused by hepatitis C virus (HCV), mainly through blood transfusion or blood products, hemodialysis, plasmapheresis and transfusion of blood cells, kidney transplantation, intravenous drug injection, sexual transmission, mother-to-child transmission, etc. . Hepatitis C is widely distributed and is more likely to develop into chronic, cirrhosis and liver cancer. The transmission routes of HCV mainly include: blood transmission, sexual transmission, mother-to-child transmission, and the transmission routes of some HCV-infected patients are not clear. [0003] An effective vaccine to prevent hepatitis C has not been successfully developed so far. Therefore, the effective way to prevent and treat hepatitis C is early diagnosis a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/576G01N33/569G01N33/58G01N21/76
CPCG01N21/76G01N33/56983G01N33/5767G01N33/577G01N33/581G01N33/6854
Inventor 王胜岚曾晓君黄思梅
Owner 中山生物工程有限公司
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